Slipper Limpet (<i>Crepidula fornicata)</i> Shells Support In Vitro Osteogenesis of Human Adipose-Derived Stem Cells
This study aimed to investigate a cost-effective alternative to man-made calcium phosphate ceramics for treating bone defects. The slipper limpet is an invasive species in European coastal waters, and its shells composed of calcium carbonate could potentially be a cost-effective source of bone graft...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/4/248 |
| _version_ | 1797604605376331776 |
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| author | Arianna De Mori Umoru Junior Alasa Alex Mühlhölzl Gordon Blunn |
| author_facet | Arianna De Mori Umoru Junior Alasa Alex Mühlhölzl Gordon Blunn |
| author_sort | Arianna De Mori |
| collection | DOAJ |
| description | This study aimed to investigate a cost-effective alternative to man-made calcium phosphate ceramics for treating bone defects. The slipper limpet is an invasive species in European coastal waters, and its shells composed of calcium carbonate could potentially be a cost-effective source of bone graft substitutes. This research analyzed the mantle of the slipper limpet (<i>Crepidula fornicata)</i> shells to enhance in vitro bone formation. Discs machined from the mantle of <i>C. fornicata</i> were analyzed using scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS), X-ray crystallography (XRD), Fourier-transform infrared spectroscopy (FT-IR) and profilometry. Calcium release and bioactivity were also studied. Cell attachment, proliferation, and osteoblastic differentiation (RT-qPCR and alkaline phosphatase activity) were measured in human adipose-derived stem cells grown on the mantle surface. The mantle material was mainly composed of aragonite and showed a sustained Ca<sup>2+</sup> release at physiological pH. In addition, apatite formation was observed in simulated body fluid after three weeks, and the materials supported osteoblastic differentiation. Overall, our findings suggest the mantle of <i>C. fornicata</i> shows potential as a material for fabricating bone graft substitutes and structural biomaterials for bone regeneration. |
| first_indexed | 2024-03-11T04:49:07Z |
| format | Article |
| id | doaj.art-79b84cc002b348ca8c6a5fcec5090f61 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T04:49:07Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-79b84cc002b348ca8c6a5fcec5090f612023-11-17T20:10:34ZengMDPI AGMarine Drugs1660-33972023-04-0121424810.3390/md21040248Slipper Limpet (<i>Crepidula fornicata)</i> Shells Support In Vitro Osteogenesis of Human Adipose-Derived Stem CellsArianna De Mori0Umoru Junior Alasa1Alex Mühlhölzl2Gordon Blunn3School of Pharmacy and Biomedical Science, University of Portsmouth, St. Michael’s Building, White Swan Road, Portsmouth PO1 2DT, UKSchool of Pharmacy and Biomedical Science, University of Portsmouth, St. Michael’s Building, White Swan Road, Portsmouth PO1 2DT, UKMikota Ltd., Pembroke Dock, Pembrokeshire, Wales SA72 6AE, UKSchool of Pharmacy and Biomedical Science, University of Portsmouth, St. Michael’s Building, White Swan Road, Portsmouth PO1 2DT, UKThis study aimed to investigate a cost-effective alternative to man-made calcium phosphate ceramics for treating bone defects. The slipper limpet is an invasive species in European coastal waters, and its shells composed of calcium carbonate could potentially be a cost-effective source of bone graft substitutes. This research analyzed the mantle of the slipper limpet (<i>Crepidula fornicata)</i> shells to enhance in vitro bone formation. Discs machined from the mantle of <i>C. fornicata</i> were analyzed using scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS), X-ray crystallography (XRD), Fourier-transform infrared spectroscopy (FT-IR) and profilometry. Calcium release and bioactivity were also studied. Cell attachment, proliferation, and osteoblastic differentiation (RT-qPCR and alkaline phosphatase activity) were measured in human adipose-derived stem cells grown on the mantle surface. The mantle material was mainly composed of aragonite and showed a sustained Ca<sup>2+</sup> release at physiological pH. In addition, apatite formation was observed in simulated body fluid after three weeks, and the materials supported osteoblastic differentiation. Overall, our findings suggest the mantle of <i>C. fornicata</i> shows potential as a material for fabricating bone graft substitutes and structural biomaterials for bone regeneration.https://www.mdpi.com/1660-3397/21/4/248<i>Crepidula fornicata</i>slipper limpet shellsmantlecalcium carbonatemesenchymal stem cellsosteogenesis |
| spellingShingle | Arianna De Mori Umoru Junior Alasa Alex Mühlhölzl Gordon Blunn Slipper Limpet (<i>Crepidula fornicata)</i> Shells Support In Vitro Osteogenesis of Human Adipose-Derived Stem Cells Marine Drugs <i>Crepidula fornicata</i> slipper limpet shells mantle calcium carbonate mesenchymal stem cells osteogenesis |
| title | Slipper Limpet (<i>Crepidula fornicata)</i> Shells Support In Vitro Osteogenesis of Human Adipose-Derived Stem Cells |
| title_full | Slipper Limpet (<i>Crepidula fornicata)</i> Shells Support In Vitro Osteogenesis of Human Adipose-Derived Stem Cells |
| title_fullStr | Slipper Limpet (<i>Crepidula fornicata)</i> Shells Support In Vitro Osteogenesis of Human Adipose-Derived Stem Cells |
| title_full_unstemmed | Slipper Limpet (<i>Crepidula fornicata)</i> Shells Support In Vitro Osteogenesis of Human Adipose-Derived Stem Cells |
| title_short | Slipper Limpet (<i>Crepidula fornicata)</i> Shells Support In Vitro Osteogenesis of Human Adipose-Derived Stem Cells |
| title_sort | slipper limpet i crepidula fornicata i shells support in vitro osteogenesis of human adipose derived stem cells |
| topic | <i>Crepidula fornicata</i> slipper limpet shells mantle calcium carbonate mesenchymal stem cells osteogenesis |
| url | https://www.mdpi.com/1660-3397/21/4/248 |
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