Methyltransferase like 21C interaction with Hsc70 affects chicken myoblast differentiation
Skeletal muscle has an essential role in boiler production, which depends on the muscle fibre development during the embryonic stage. Whereas the posttranslational modifications of both histone and non-histone proteins are known to regulate postnatal muscle development, the biological significance o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/1828051X.2023.2222754 |
| _version_ | 1827276577849737216 |
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| author | Jia Cheng Ruiqing Li Ling Wang Tao Zhang Guoqiang Yuan Hongzhao Lu |
| author_facet | Jia Cheng Ruiqing Li Ling Wang Tao Zhang Guoqiang Yuan Hongzhao Lu |
| author_sort | Jia Cheng |
| collection | DOAJ |
| description | Skeletal muscle has an essential role in boiler production, which depends on the muscle fibre development during the embryonic stage. Whereas the posttranslational modifications of both histone and non-histone proteins are known to regulate postnatal muscle development, the biological significance of non-histone methylation is poorly understood. Recently, the methyltransferase like 21 C (METTL21C) is identified as a non-histone lysine methyltransferase whose role in chicken muscle function remains elusive. In this study, METTL21C was found by comparing free-range and caged modes in Lueyang black-boned chicken aged 60 and 120 days. Quantitative real-time PCR (qPCR) analysis demonstrated that METTL21C was mainly enriched in chicken soleus (SOL) and gastrocnemius muscle (GA) (p < .05). Meanwhile, METTL21C had a rapid increase during chicken myoblast differentiation. After METTL21C overexpression, myogenic differentiation (MyoD) and myogenin (MyoG) expression were significantly increased, indicating that METTL21C promotes chicken myoblast differentiation. Using co-immunoprecipitation (Co-IP) and liquid chromatography-mass spectrometry (LC-MS/MS) analysis, heat shock cognate 71 kDa protein (Hsc70) was confirmed to interact with METTL21C, while the effects of Hsc70 Lys-561 (Hsc70 K561) mutation suggested that Hsc70 was a METTL21C target protein. These results identify the molecular mechanism of METTL21C interaction with Hsc70 associated with chicken myoblast differentiation in the postnatal period.Highlights The mRNA of methyltransferase like 21C (METTL21C) was significantly increased in free-rang mode and during the chicken myoblast differentiation. Overexpression of METTL21C promoted the primary chicken myoblasts proliferation and differentiation. METTL21C could mediate the lysine methylation modification of heat shock cognate 71 kDa protein (Hsc70) to regulate the proliferation of chicken myoblasts. |
| first_indexed | 2024-03-08T16:59:58Z |
| format | Article |
| id | doaj.art-79b93bc4688442c0ab148d8731ed6cd6 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-04-24T06:59:49Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-79b93bc4688442c0ab148d8731ed6cd62024-04-22T08:52:28ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2023-12-0122160561410.1080/1828051X.2023.22227542222754Methyltransferase like 21C interaction with Hsc70 affects chicken myoblast differentiationJia Cheng0Ruiqing Li1Ling Wang2Tao Zhang3Guoqiang Yuan4Hongzhao Lu5College of Biological Science and Engineering, Shaanxi University of TechnologyCollege of Biological Science and Engineering, Shaanxi University of TechnologyCollege of Biological Science and Engineering, Shaanxi University of TechnologyCollege of Biological Science and Engineering, Shaanxi University of TechnologyShaanxi Baiweiyuan Network Technology Co., LTDCollege of Biological Science and Engineering, Shaanxi University of TechnologySkeletal muscle has an essential role in boiler production, which depends on the muscle fibre development during the embryonic stage. Whereas the posttranslational modifications of both histone and non-histone proteins are known to regulate postnatal muscle development, the biological significance of non-histone methylation is poorly understood. Recently, the methyltransferase like 21 C (METTL21C) is identified as a non-histone lysine methyltransferase whose role in chicken muscle function remains elusive. In this study, METTL21C was found by comparing free-range and caged modes in Lueyang black-boned chicken aged 60 and 120 days. Quantitative real-time PCR (qPCR) analysis demonstrated that METTL21C was mainly enriched in chicken soleus (SOL) and gastrocnemius muscle (GA) (p < .05). Meanwhile, METTL21C had a rapid increase during chicken myoblast differentiation. After METTL21C overexpression, myogenic differentiation (MyoD) and myogenin (MyoG) expression were significantly increased, indicating that METTL21C promotes chicken myoblast differentiation. Using co-immunoprecipitation (Co-IP) and liquid chromatography-mass spectrometry (LC-MS/MS) analysis, heat shock cognate 71 kDa protein (Hsc70) was confirmed to interact with METTL21C, while the effects of Hsc70 Lys-561 (Hsc70 K561) mutation suggested that Hsc70 was a METTL21C target protein. These results identify the molecular mechanism of METTL21C interaction with Hsc70 associated with chicken myoblast differentiation in the postnatal period.Highlights The mRNA of methyltransferase like 21C (METTL21C) was significantly increased in free-rang mode and during the chicken myoblast differentiation. Overexpression of METTL21C promoted the primary chicken myoblasts proliferation and differentiation. METTL21C could mediate the lysine methylation modification of heat shock cognate 71 kDa protein (Hsc70) to regulate the proliferation of chicken myoblasts.http://dx.doi.org/10.1080/1828051X.2023.2222754chickenmettl21chsc70myoblastdifferentiation |
| spellingShingle | Jia Cheng Ruiqing Li Ling Wang Tao Zhang Guoqiang Yuan Hongzhao Lu Methyltransferase like 21C interaction with Hsc70 affects chicken myoblast differentiation Italian Journal of Animal Science chicken mettl21c hsc70 myoblast differentiation |
| title | Methyltransferase like 21C interaction with Hsc70 affects chicken myoblast differentiation |
| title_full | Methyltransferase like 21C interaction with Hsc70 affects chicken myoblast differentiation |
| title_fullStr | Methyltransferase like 21C interaction with Hsc70 affects chicken myoblast differentiation |
| title_full_unstemmed | Methyltransferase like 21C interaction with Hsc70 affects chicken myoblast differentiation |
| title_short | Methyltransferase like 21C interaction with Hsc70 affects chicken myoblast differentiation |
| title_sort | methyltransferase like 21c interaction with hsc70 affects chicken myoblast differentiation |
| topic | chicken mettl21c hsc70 myoblast differentiation |
| url | http://dx.doi.org/10.1080/1828051X.2023.2222754 |
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