Salicylic Acid Is Part of the Mi-1-Mediated Defense Response to Root-Knot Nematode in Tomato
The Mi-1 gene of tomato confers resistance against three species of root-knot nematode in tomato (Lycopersicon esculentum). Transformation of tomato carrying Mi-1 with a construct expressing NahG, which encodes salicylate hydroxylase, a bacterial enzyme that degrades salicylic acid (SA) to catechol,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2004-04-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.4.351 |
| _version_ | 1811287304244822016 |
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| author | Craig Branch Chin-Feng Hwang Duroy A. Navarre Valerie M. Williamson |
| author_facet | Craig Branch Chin-Feng Hwang Duroy A. Navarre Valerie M. Williamson |
| author_sort | Craig Branch |
| collection | DOAJ |
| description | The Mi-1 gene of tomato confers resistance against three species of root-knot nematode in tomato (Lycopersicon esculentum). Transformation of tomato carrying Mi-1 with a construct expressing NahG, which encodes salicylate hydroxylase, a bacterial enzyme that degrades salicylic acid (SA) to catechol, results in partial loss of resistance to root-knot nematodes. Exogenous SA was toxic to roots expressing NahG but not to control roots. This toxicity is most likely due to the production of catechol from SA, and we report here that 100 μM catechol is toxic to tomato roots. Benzothiadiazole, a SA analog, completely restores nematode resistance in Mi-1 roots transformed with NahG but does not confer resistance to susceptible tomato roots. The localized cell death produced by transient expression in Nicotiana benthamiana of Mi-DS4, a constitutively lethal chimera of Mi-1 with one of its homologs, was prevented by coexpression of NahG. These results indicate that SA is an important component of the signaling that leads to nematode resistance and the associated hypersensitive response. |
| first_indexed | 2024-04-13T03:15:19Z |
| format | Article |
| id | doaj.art-79ba530b5c4d46d28047fc3a20433360 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:15:19Z |
| publishDate | 2004-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-79ba530b5c4d46d28047fc3a204333602022-12-22T03:04:55ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062004-04-0117435135610.1094/MPMI.2004.17.4.351Salicylic Acid Is Part of the Mi-1-Mediated Defense Response to Root-Knot Nematode in TomatoCraig BranchChin-Feng HwangDuroy A. NavarreValerie M. WilliamsonThe Mi-1 gene of tomato confers resistance against three species of root-knot nematode in tomato (Lycopersicon esculentum). Transformation of tomato carrying Mi-1 with a construct expressing NahG, which encodes salicylate hydroxylase, a bacterial enzyme that degrades salicylic acid (SA) to catechol, results in partial loss of resistance to root-knot nematodes. Exogenous SA was toxic to roots expressing NahG but not to control roots. This toxicity is most likely due to the production of catechol from SA, and we report here that 100 μM catechol is toxic to tomato roots. Benzothiadiazole, a SA analog, completely restores nematode resistance in Mi-1 roots transformed with NahG but does not confer resistance to susceptible tomato roots. The localized cell death produced by transient expression in Nicotiana benthamiana of Mi-DS4, a constitutively lethal chimera of Mi-1 with one of its homologs, was prevented by coexpression of NahG. These results indicate that SA is an important component of the signaling that leads to nematode resistance and the associated hypersensitive response.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.4.351 |
| spellingShingle | Craig Branch Chin-Feng Hwang Duroy A. Navarre Valerie M. Williamson Salicylic Acid Is Part of the Mi-1-Mediated Defense Response to Root-Knot Nematode in Tomato Molecular Plant-Microbe Interactions |
| title | Salicylic Acid Is Part of the Mi-1-Mediated Defense Response to Root-Knot Nematode in Tomato |
| title_full | Salicylic Acid Is Part of the Mi-1-Mediated Defense Response to Root-Knot Nematode in Tomato |
| title_fullStr | Salicylic Acid Is Part of the Mi-1-Mediated Defense Response to Root-Knot Nematode in Tomato |
| title_full_unstemmed | Salicylic Acid Is Part of the Mi-1-Mediated Defense Response to Root-Knot Nematode in Tomato |
| title_short | Salicylic Acid Is Part of the Mi-1-Mediated Defense Response to Root-Knot Nematode in Tomato |
| title_sort | salicylic acid is part of the mi 1 mediated defense response to root knot nematode in tomato |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.4.351 |
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