Longitudinal monitoring of individual infection progression in Drosophila melanogaster
Summary: The innate immune system is critical for infection survival. Drosophila melanogaster is a key model for understanding the evolution and dynamics of innate immunity. Current toolsets for fly infection studies are limited in throughput and, because of their destructive nature, cannot generate...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222016509 |
| _version_ | 1811255782243565568 |
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| author | Bryan A. Ramirez-Corona Anna C. Love Srikiran Chandrasekaran Jennifer A. Prescher Zeba Wunderlich |
| author_facet | Bryan A. Ramirez-Corona Anna C. Love Srikiran Chandrasekaran Jennifer A. Prescher Zeba Wunderlich |
| author_sort | Bryan A. Ramirez-Corona |
| collection | DOAJ |
| description | Summary: The innate immune system is critical for infection survival. Drosophila melanogaster is a key model for understanding the evolution and dynamics of innate immunity. Current toolsets for fly infection studies are limited in throughput and, because of their destructive nature, cannot generate longitudinal measurements in individual animals. We report a bioluminescent imaging strategy enabling non-invasive characterization of pathogen load. By using Escherichia coli expressing the ilux operon, we demonstrate that photon flux from autobioluminescent bacteria can be used to monitor pathogen loads in individual, living flies. Because animal sacrifice is not necessary to estimate pathogen load, stochastic responses to infection can be characterized in individuals over time. The high temporal resolution of bioluminescence imaging enables visualization of the dynamics of microbial clearance on the hours time-scale. This non-invasive imaging strategy provides a simple and scalable platform to observe changes in pathogen load in vivo over time. |
| first_indexed | 2024-04-12T17:30:19Z |
| format | Article |
| id | doaj.art-79bafaa1675147bda5b79a15cfb33f6f |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T17:30:19Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-79bafaa1675147bda5b79a15cfb33f6f2022-12-22T03:23:10ZengElsevieriScience2589-00422022-11-012511105378Longitudinal monitoring of individual infection progression in Drosophila melanogasterBryan A. Ramirez-Corona0Anna C. Love1Srikiran Chandrasekaran2Jennifer A. Prescher3Zeba Wunderlich4Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA92697, USADepartment of Chemistry, University of California, Irvine, Irvine, CA92697, USACenter for Complex Biological Sciences, University of California, Irvine, Irvine, CA92697, USADepartment of Chemistry, University of California, Irvine, Irvine, CA92697, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA92697, USA; Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA92697, USADepartment of Developmental and Cell Biology, University of California, Irvine, Irvine, CA92697, USA; Department of Biology, Boston University, Boston, MA02215, USA; Biological Design Center, Boston University, Boston, MA02215, USA; Corresponding authorSummary: The innate immune system is critical for infection survival. Drosophila melanogaster is a key model for understanding the evolution and dynamics of innate immunity. Current toolsets for fly infection studies are limited in throughput and, because of their destructive nature, cannot generate longitudinal measurements in individual animals. We report a bioluminescent imaging strategy enabling non-invasive characterization of pathogen load. By using Escherichia coli expressing the ilux operon, we demonstrate that photon flux from autobioluminescent bacteria can be used to monitor pathogen loads in individual, living flies. Because animal sacrifice is not necessary to estimate pathogen load, stochastic responses to infection can be characterized in individuals over time. The high temporal resolution of bioluminescence imaging enables visualization of the dynamics of microbial clearance on the hours time-scale. This non-invasive imaging strategy provides a simple and scalable platform to observe changes in pathogen load in vivo over time.http://www.sciencedirect.com/science/article/pii/S2589004222016509Optical imagingMicrobiology |
| spellingShingle | Bryan A. Ramirez-Corona Anna C. Love Srikiran Chandrasekaran Jennifer A. Prescher Zeba Wunderlich Longitudinal monitoring of individual infection progression in Drosophila melanogaster iScience Optical imaging Microbiology |
| title | Longitudinal monitoring of individual infection progression in Drosophila melanogaster |
| title_full | Longitudinal monitoring of individual infection progression in Drosophila melanogaster |
| title_fullStr | Longitudinal monitoring of individual infection progression in Drosophila melanogaster |
| title_full_unstemmed | Longitudinal monitoring of individual infection progression in Drosophila melanogaster |
| title_short | Longitudinal monitoring of individual infection progression in Drosophila melanogaster |
| title_sort | longitudinal monitoring of individual infection progression in drosophila melanogaster |
| topic | Optical imaging Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222016509 |
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