The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens

It was postulated that a phytobiotic preparation containing cinnamon oil and citric acid added to drinking water for chickens in a suitable amount and for a suitable time would beneficially modify the microbiota composition and morphology of the small intestine, thereby improving immunity and growth...

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Main Authors: Magdalena Krauze, Monika Cendrowska-Pinkosz, Paulius Matuseviĉius, Anna Stępniowska, Paweł Jurczak, Katarzyna Ognik
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Animals
Subjects:
Online Access:https://www.mdpi.com/2076-2615/11/2/399
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author Magdalena Krauze
Monika Cendrowska-Pinkosz
Paulius Matuseviĉius
Anna Stępniowska
Paweł Jurczak
Katarzyna Ognik
author_facet Magdalena Krauze
Monika Cendrowska-Pinkosz
Paulius Matuseviĉius
Anna Stępniowska
Paweł Jurczak
Katarzyna Ognik
author_sort Magdalena Krauze
collection DOAJ
description It was postulated that a phytobiotic preparation containing cinnamon oil and citric acid added to drinking water for chickens in a suitable amount and for a suitable time would beneficially modify the microbiota composition and morphology of the small intestine, thereby improving immunity and growth performance without inducing metabolic disorders. The aim of the study was to establish the dosage and time of administration of such a phytobiotic that would have the most beneficial effect on the intestinal histology and microbiota, production results, and immune and metabolic status of broiler chickens. The experiment was carried out on 980 one-day-old male chickens until the age of 42 days. The chickens were assigned to seven experimental groups of 140 birds each (seven replications of 20 individuals each). The control group (G-C) did not receive the phytobiotic. Groups CT-0.05, CT-0.1, and CT-0.25 received the phytobiotic in their drinking water in the amount of 0.05, 0.1, and 0.2 mL/L, respectively, at days 1–42 of life (continuous application, CT). The birds in groups PT-0.05, PT-0.5, and PT-0.25 received the phytobiotic in the same amounts, but only at days 1–7, 15–21, and 29–35 of life (periodic application, PT). Selected antioxidant and biochemical parameters were determined in the blood of the chickens, as well as parameters of immune status and redox status. The morphology of the intestinal epithelium, composition of the microbiome, and production parameters of chickens receiving the phytobiotic in their drinking water were determined as well. The addition of a phytobiotic containing cinnamon oil and citric acid to the drinking water of broiler chickens at a suitable dosage and for a suitable time can beneficially modify the microbiome composition and morphometry of the small intestine (total number of fungi <i>p</i> < 0.001, total number of aerobic bacteria <i>p</i> < 0.001; and total number of coliform bacteria <i>p</i> < 0.001 was decreased) improving the immunity and growth performance of the chickens (there occurred a villi lengthening <i>p</i> = 0.002 and crypts deepening <i>p</i> = 0.003). Among the three tested dosages (0.05, 0.1, and 0.25 mL/L of water) of the preparation containing cinnamon oil, the dosage of 0.25 mL/L of water administered for 42 days proved to be most beneficial. Chickens receiving the phytobiotic in the amount of 0.25 mL/L had better growth performance, which was linked to the beneficial effect of the preparation on the microbiome of the small intestine, metabolism (the HDL level <i>p</i> = 0.017 was increased; and a decreased level of total cholesterol (TC) <i>p</i> = 0.018 and nonesterified fatty acids (NEFA) <i>p</i> = 0.007, LDL <i>p</i> = 0.041, as well as triacylglycerols (TAG) <i>p</i> = 0.014), and immune (the level of lysozyme <i>p</i> = 0.041 was increased, as well as the percentage of phagocytic cells <i>p</i> = 0.034, phagocytosis index <i>p</i> = 0.038, and Ig-A level <i>p</i> = 0.031) and antioxidant system (the level of LOOH <i>p</i> < 0.001, MDA <i>p</i> = 0.002, and the activity of Catalase (CAT) <i>p</i> < 0.001 were decreased, but the level of ferric reducing ability of plasma (FRAP) <i>p</i> = 0.029, glutathione <i>p</i> = 0.045 and vitamin C <i>p</i> = 0.021 were increased).
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spelling doaj.art-79bce5ae7bd8496a967f0b0505c62e252023-12-03T12:26:45ZengMDPI AGAnimals2076-26152021-02-0111239910.3390/ani11020399The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler ChickensMagdalena Krauze0Monika Cendrowska-Pinkosz1Paulius Matuseviĉius2Anna Stępniowska3Paweł Jurczak4Katarzyna Ognik5Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bio-Economy, University of Life Sciences in Lublin, Akademicka st. 13, 20-950 Lublin, PolandChair and Department of Human Anatomy, Medical University of Lublin, 20-090 Lublin, PolandDepartment of Animal Nutrition, Lithuanian University of Health, Kaunas, Tilzes 18, LT-47181 Kaunas, LithuaniaDepartment of Biochemistry and Toxicology, Faculty of Animal Sciences and Bio-Economy, University of Life Sciences in Lublin, Akademicka st. 13, 20-950 Lublin, PolandDepartment of Biochemistry and Toxicology, Faculty of Animal Sciences and Bio-Economy, University of Life Sciences in Lublin, Akademicka st. 13, 20-950 Lublin, PolandDepartment of Biochemistry and Toxicology, Faculty of Animal Sciences and Bio-Economy, University of Life Sciences in Lublin, Akademicka st. 13, 20-950 Lublin, PolandIt was postulated that a phytobiotic preparation containing cinnamon oil and citric acid added to drinking water for chickens in a suitable amount and for a suitable time would beneficially modify the microbiota composition and morphology of the small intestine, thereby improving immunity and growth performance without inducing metabolic disorders. The aim of the study was to establish the dosage and time of administration of such a phytobiotic that would have the most beneficial effect on the intestinal histology and microbiota, production results, and immune and metabolic status of broiler chickens. The experiment was carried out on 980 one-day-old male chickens until the age of 42 days. The chickens were assigned to seven experimental groups of 140 birds each (seven replications of 20 individuals each). The control group (G-C) did not receive the phytobiotic. Groups CT-0.05, CT-0.1, and CT-0.25 received the phytobiotic in their drinking water in the amount of 0.05, 0.1, and 0.2 mL/L, respectively, at days 1–42 of life (continuous application, CT). The birds in groups PT-0.05, PT-0.5, and PT-0.25 received the phytobiotic in the same amounts, but only at days 1–7, 15–21, and 29–35 of life (periodic application, PT). Selected antioxidant and biochemical parameters were determined in the blood of the chickens, as well as parameters of immune status and redox status. The morphology of the intestinal epithelium, composition of the microbiome, and production parameters of chickens receiving the phytobiotic in their drinking water were determined as well. The addition of a phytobiotic containing cinnamon oil and citric acid to the drinking water of broiler chickens at a suitable dosage and for a suitable time can beneficially modify the microbiome composition and morphometry of the small intestine (total number of fungi <i>p</i> < 0.001, total number of aerobic bacteria <i>p</i> < 0.001; and total number of coliform bacteria <i>p</i> < 0.001 was decreased) improving the immunity and growth performance of the chickens (there occurred a villi lengthening <i>p</i> = 0.002 and crypts deepening <i>p</i> = 0.003). Among the three tested dosages (0.05, 0.1, and 0.25 mL/L of water) of the preparation containing cinnamon oil, the dosage of 0.25 mL/L of water administered for 42 days proved to be most beneficial. Chickens receiving the phytobiotic in the amount of 0.25 mL/L had better growth performance, which was linked to the beneficial effect of the preparation on the microbiome of the small intestine, metabolism (the HDL level <i>p</i> = 0.017 was increased; and a decreased level of total cholesterol (TC) <i>p</i> = 0.018 and nonesterified fatty acids (NEFA) <i>p</i> = 0.007, LDL <i>p</i> = 0.041, as well as triacylglycerols (TAG) <i>p</i> = 0.014), and immune (the level of lysozyme <i>p</i> = 0.041 was increased, as well as the percentage of phagocytic cells <i>p</i> = 0.034, phagocytosis index <i>p</i> = 0.038, and Ig-A level <i>p</i> = 0.031) and antioxidant system (the level of LOOH <i>p</i> < 0.001, MDA <i>p</i> = 0.002, and the activity of Catalase (CAT) <i>p</i> < 0.001 were decreased, but the level of ferric reducing ability of plasma (FRAP) <i>p</i> = 0.029, glutathione <i>p</i> = 0.045 and vitamin C <i>p</i> = 0.021 were increased).https://www.mdpi.com/2076-2615/11/2/399phytobioticcinnamon oilnutritional supplementblood parametersmicrobiological parameters
spellingShingle Magdalena Krauze
Monika Cendrowska-Pinkosz
Paulius Matuseviĉius
Anna Stępniowska
Paweł Jurczak
Katarzyna Ognik
The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens
Animals
phytobiotic
cinnamon oil
nutritional supplement
blood parameters
microbiological parameters
title The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens
title_full The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens
title_fullStr The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens
title_full_unstemmed The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens
title_short The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens
title_sort effect of administration of a phytobiotic containing cinnamon oil and citric acid on the metabolism immunity and growth performance of broiler chickens
topic phytobiotic
cinnamon oil
nutritional supplement
blood parameters
microbiological parameters
url https://www.mdpi.com/2076-2615/11/2/399
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