Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot
Wheat (<i>Triticum aestivum</i> L.) is an important staple crop. Sharp eyespot and common root rot are destructive diseases of wheat. Antimicrobial peptides (AMPs) are small peptides with broad-spectrum antimicrobial activity. In this study, we synthesized the <i>DmAMP1W</i>...
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MDPI AG
2020-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/2/647 |
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| author | Qiang Su Ke Wang Zengyan Zhang |
| author_facet | Qiang Su Ke Wang Zengyan Zhang |
| author_sort | Qiang Su |
| collection | DOAJ |
| description | Wheat (<i>Triticum aestivum</i> L.) is an important staple crop. Sharp eyespot and common root rot are destructive diseases of wheat. Antimicrobial peptides (AMPs) are small peptides with broad-spectrum antimicrobial activity. In this study, we synthesized the <i>DmAMP1W</i> gene, encoding <i>Dahlia merckii</i> DmAMP1, and investigated the antifungal role of DmAMP1W in vitro and in transgenic wheat. Protein electrophoresis analysis and in vitro inhibition results demonstrated that the synthesized <i>DmAMP1W</i> correctly translated to the expected peptide DmAMP1W, and the purified peptide inhibited growths of the fungi <i>Rhizoctonia cerealis</i> and <i>Bipolaris sorokiniana</i>, the pathogenic causes of wheat sharp eyespot and common root rot. <i>DmAMP1W</i> was introduced into a wheat variety Zhoumai18 via <i>Agrobacterium</i>-mediated transformation. The molecular characteristics indicated that <i>DmAMP1W</i> could be heritable and expressed in five transgenic wheat lines in T<sub>1</sub>−T<sub>2</sub> generations. Average sharp eyespot infection types of these five <i>DmAMP1W</i> transgenic wheat lines in T<sub>1</sub>−T<sub>2</sub> generations decreased 0.69−1.54 and 0.40−0.82 compared with non-transformed Zhoumai18, respectively. Average common root rot infection types of these transgenic lines and non-transformed Zhoumai18 were 1.23−1.48 and 2.27, respectively. These results indicated that DmAMP1W-expressing transgenic wheat lines displayed enhanced-resistance to both sharp eyespot and common root rot. This study provides new broad-spectrum antifungal resources for wheat breeding. |
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| spelling | doaj.art-79bd4814af9b4b0485e1e230e4ae6adf2022-12-22T02:59:45ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121264710.3390/ijms21020647ijms21020647Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root RotQiang Su0Ke Wang1Zengyan Zhang2The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaWheat (<i>Triticum aestivum</i> L.) is an important staple crop. Sharp eyespot and common root rot are destructive diseases of wheat. Antimicrobial peptides (AMPs) are small peptides with broad-spectrum antimicrobial activity. In this study, we synthesized the <i>DmAMP1W</i> gene, encoding <i>Dahlia merckii</i> DmAMP1, and investigated the antifungal role of DmAMP1W in vitro and in transgenic wheat. Protein electrophoresis analysis and in vitro inhibition results demonstrated that the synthesized <i>DmAMP1W</i> correctly translated to the expected peptide DmAMP1W, and the purified peptide inhibited growths of the fungi <i>Rhizoctonia cerealis</i> and <i>Bipolaris sorokiniana</i>, the pathogenic causes of wheat sharp eyespot and common root rot. <i>DmAMP1W</i> was introduced into a wheat variety Zhoumai18 via <i>Agrobacterium</i>-mediated transformation. The molecular characteristics indicated that <i>DmAMP1W</i> could be heritable and expressed in five transgenic wheat lines in T<sub>1</sub>−T<sub>2</sub> generations. Average sharp eyespot infection types of these five <i>DmAMP1W</i> transgenic wheat lines in T<sub>1</sub>−T<sub>2</sub> generations decreased 0.69−1.54 and 0.40−0.82 compared with non-transformed Zhoumai18, respectively. Average common root rot infection types of these transgenic lines and non-transformed Zhoumai18 were 1.23−1.48 and 2.27, respectively. These results indicated that DmAMP1W-expressing transgenic wheat lines displayed enhanced-resistance to both sharp eyespot and common root rot. This study provides new broad-spectrum antifungal resources for wheat breeding.https://www.mdpi.com/1422-0067/21/2/647antimicrobial peptidesdmamp1wfungal resistance<i>rhizoctonia cerealis</i>wheat |
| spellingShingle | Qiang Su Ke Wang Zengyan Zhang Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot International Journal of Molecular Sciences antimicrobial peptides dmamp1w fungal resistance <i>rhizoctonia cerealis</i> wheat |
| title | Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot |
| title_full | Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot |
| title_fullStr | Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot |
| title_full_unstemmed | Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot |
| title_short | Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot |
| title_sort | ecotopic expression of the antimicrobial peptide dmamp1w improves resistance of transgenic wheat to two diseases sharp eyespot and common root rot |
| topic | antimicrobial peptides dmamp1w fungal resistance <i>rhizoctonia cerealis</i> wheat |
| url | https://www.mdpi.com/1422-0067/21/2/647 |
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