Excess glucose or fat differentially affects metabolism and appetite-related gene expression during zebrafish embryogenesis
Summary: Zebrafish embryos use their yolk sac reserve as the sole nutrient source during embryogenesis. The two main forms of energy fuel can be found in the form of glucose or fat. Zebrafish embryos were exposed to glucose or injected with free fatty acid/Triacylglycerol (FFA/TAG) into the yolk sac...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223011409 |
| _version_ | 1797774553447923712 |
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| author | Bridget Konadu Carol K. Cox Michael R. Garrett Yann Gibert |
| author_facet | Bridget Konadu Carol K. Cox Michael R. Garrett Yann Gibert |
| author_sort | Bridget Konadu |
| collection | DOAJ |
| description | Summary: Zebrafish embryos use their yolk sac reserve as the sole nutrient source during embryogenesis. The two main forms of energy fuel can be found in the form of glucose or fat. Zebrafish embryos were exposed to glucose or injected with free fatty acid/Triacylglycerol (FFA/TAG) into the yolk sac at 24 hpf. At 72 hpf, glucose exposed or FFA/TAG injected had differential effects on gene expression in embryos, with fat activating lipolysis and β-oxidation and glucose activating the insulin pathway. Bulk RNA-seq revealed that more gene expression was affected by glucose exposure compared to FFA/TAGs injection. Appetite-controlling genes were also differently affected by glucose exposure or FFA/TAG injections. Because the embryo did not yet feed itself at the time of our analysis, gene expression changes occurred in absence of actual hunger and revealed how the embryo manages its nutrient intake before active feeding. |
| first_indexed | 2024-03-12T22:22:44Z |
| format | Article |
| id | doaj.art-79bd7d1abd8f467ebc14b82c035d9d3a |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:22:44Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-79bd7d1abd8f467ebc14b82c035d9d3a2023-07-23T04:55:23ZengElsevieriScience2589-00422023-07-01267107063Excess glucose or fat differentially affects metabolism and appetite-related gene expression during zebrafish embryogenesisBridget Konadu0Carol K. Cox1Michael R. Garrett2Yann Gibert3Department of Cell and Molecular Biology, Cancer Center and Research Institute, University of Mississippi Medical Center, Jackson, MS 39216, USADepartment of Cell and Molecular Biology, Cancer Center and Research Institute, University of Mississippi Medical Center, Jackson, MS 39216, USADepartment of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, MS 39216, USADepartment of Cell and Molecular Biology, Cancer Center and Research Institute, University of Mississippi Medical Center, Jackson, MS 39216, USA; Corresponding authorSummary: Zebrafish embryos use their yolk sac reserve as the sole nutrient source during embryogenesis. The two main forms of energy fuel can be found in the form of glucose or fat. Zebrafish embryos were exposed to glucose or injected with free fatty acid/Triacylglycerol (FFA/TAG) into the yolk sac at 24 hpf. At 72 hpf, glucose exposed or FFA/TAG injected had differential effects on gene expression in embryos, with fat activating lipolysis and β-oxidation and glucose activating the insulin pathway. Bulk RNA-seq revealed that more gene expression was affected by glucose exposure compared to FFA/TAGs injection. Appetite-controlling genes were also differently affected by glucose exposure or FFA/TAG injections. Because the embryo did not yet feed itself at the time of our analysis, gene expression changes occurred in absence of actual hunger and revealed how the embryo manages its nutrient intake before active feeding.http://www.sciencedirect.com/science/article/pii/S2589004223011409EmbryologyTranscriptomicsModel organism |
| spellingShingle | Bridget Konadu Carol K. Cox Michael R. Garrett Yann Gibert Excess glucose or fat differentially affects metabolism and appetite-related gene expression during zebrafish embryogenesis iScience Embryology Transcriptomics Model organism |
| title | Excess glucose or fat differentially affects metabolism and appetite-related gene expression during zebrafish embryogenesis |
| title_full | Excess glucose or fat differentially affects metabolism and appetite-related gene expression during zebrafish embryogenesis |
| title_fullStr | Excess glucose or fat differentially affects metabolism and appetite-related gene expression during zebrafish embryogenesis |
| title_full_unstemmed | Excess glucose or fat differentially affects metabolism and appetite-related gene expression during zebrafish embryogenesis |
| title_short | Excess glucose or fat differentially affects metabolism and appetite-related gene expression during zebrafish embryogenesis |
| title_sort | excess glucose or fat differentially affects metabolism and appetite related gene expression during zebrafish embryogenesis |
| topic | Embryology Transcriptomics Model organism |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223011409 |
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