Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac Hypertrophy
Cardiac hypertrophy is an adaptive cardiac response that accommodates the variable hemodynamic demands of the human body during extended periods of preload or afterload increase. In recent years, an increasing number of studies have pointed to a potential connection between myocardial hypertrophy an...
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.781676/full |
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| author | Yang-Hao Chen Ling-Feng Zhong Xia Hong Qian-Li Zhu Song-Jie Wang Ji-Bo Han Wei-Jian Huang Bo-Zhi Ye |
| author_facet | Yang-Hao Chen Ling-Feng Zhong Xia Hong Qian-Li Zhu Song-Jie Wang Ji-Bo Han Wei-Jian Huang Bo-Zhi Ye |
| author_sort | Yang-Hao Chen |
| collection | DOAJ |
| description | Cardiac hypertrophy is an adaptive cardiac response that accommodates the variable hemodynamic demands of the human body during extended periods of preload or afterload increase. In recent years, an increasing number of studies have pointed to a potential connection between myocardial hypertrophy and abnormal expression of non-coding RNAs. Circular RNA (circRNA), as one of the non-coding RNAs, plays an essential role in cardiac hypertrophy. However, few studies have systematically analyzed circRNA-related competing endogenous RNA (ceRNA) regulatory networks associated with cardiac hypertrophy. Therefore, we used public databases from online prediction websites to predict and screen differentially expressed mRNAs and miRNAs and ultimately obtained circRNAs related to cardiac hypertrophy. Based on this result, we went on to establish a circRNAs-related ceRNA regulatory network. This study is the first to establish a circRNA-mediated ceRNA regulatory network associated with myocardial hypertrophy. To verify the results of our analysis, we used PCR to verify the differentially expressed mRNAs and miRNAs in animal myocardial hypertrophy model samples. Our findings suggest that three mRNAs (Col12a1, Thbs1, and Tgfbr3), four miRNAs (miR-20a-5p, miR-27b-3p, miR-342-3p, and miR-378a-3p), and four related circRNAs (circ_0002702, circ_0110609, circ_0013751, and circ_0047959) may play a key role in cardiac hypertrophy. |
| first_indexed | 2024-12-13T00:45:19Z |
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| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-13T00:45:19Z |
| publishDate | 2022-02-01 |
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| series | Frontiers in Genetics |
| spelling | doaj.art-79be381be3524a6a8addf3b741fe68ed2022-12-22T00:05:03ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-02-011310.3389/fgene.2022.781676781676Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac HypertrophyYang-Hao Chen0Ling-Feng Zhong1Xia Hong2Qian-Li Zhu3Song-Jie Wang4Ji-Bo Han5Wei-Jian Huang6Bo-Zhi Ye7The Key Laboratory of Cardiovascular Disease of Wenzhou, Department of Cardiology, The First Affiliated Hospital of WenZhou Medical University, WenZhou, ChinaThe Key Laboratory of Cardiovascular Disease of Wenzhou, Department of Cardiology, The First Affiliated Hospital of WenZhou Medical University, WenZhou, ChinaCoronary Care Unit, The First Affiliated Hospital of Wenzhou Medical University, WenZhou, ChinaThe Key Laboratory of Cardiovascular Disease of Wenzhou, Department of Cardiology, The First Affiliated Hospital of WenZhou Medical University, WenZhou, ChinaThe Key Laboratory of Cardiovascular Disease of Wenzhou, Department of Cardiology, The First Affiliated Hospital of WenZhou Medical University, WenZhou, ChinaDepartment of Cardiology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, ChinaThe Key Laboratory of Cardiovascular Disease of Wenzhou, Department of Cardiology, The First Affiliated Hospital of WenZhou Medical University, WenZhou, ChinaThe Key Laboratory of Cardiovascular Disease of Wenzhou, Department of Cardiology, The First Affiliated Hospital of WenZhou Medical University, WenZhou, ChinaCardiac hypertrophy is an adaptive cardiac response that accommodates the variable hemodynamic demands of the human body during extended periods of preload or afterload increase. In recent years, an increasing number of studies have pointed to a potential connection between myocardial hypertrophy and abnormal expression of non-coding RNAs. Circular RNA (circRNA), as one of the non-coding RNAs, plays an essential role in cardiac hypertrophy. However, few studies have systematically analyzed circRNA-related competing endogenous RNA (ceRNA) regulatory networks associated with cardiac hypertrophy. Therefore, we used public databases from online prediction websites to predict and screen differentially expressed mRNAs and miRNAs and ultimately obtained circRNAs related to cardiac hypertrophy. Based on this result, we went on to establish a circRNAs-related ceRNA regulatory network. This study is the first to establish a circRNA-mediated ceRNA regulatory network associated with myocardial hypertrophy. To verify the results of our analysis, we used PCR to verify the differentially expressed mRNAs and miRNAs in animal myocardial hypertrophy model samples. Our findings suggest that three mRNAs (Col12a1, Thbs1, and Tgfbr3), four miRNAs (miR-20a-5p, miR-27b-3p, miR-342-3p, and miR-378a-3p), and four related circRNAs (circ_0002702, circ_0110609, circ_0013751, and circ_0047959) may play a key role in cardiac hypertrophy.https://www.frontiersin.org/articles/10.3389/fgene.2022.781676/fullcardiac hypertrophycompetitive endogenous RNAbioinformaticscircRNAmiRNA |
| spellingShingle | Yang-Hao Chen Ling-Feng Zhong Xia Hong Qian-Li Zhu Song-Jie Wang Ji-Bo Han Wei-Jian Huang Bo-Zhi Ye Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac Hypertrophy Frontiers in Genetics cardiac hypertrophy competitive endogenous RNA bioinformatics circRNA miRNA |
| title | Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac Hypertrophy |
| title_full | Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac Hypertrophy |
| title_fullStr | Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac Hypertrophy |
| title_full_unstemmed | Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac Hypertrophy |
| title_short | Integrated Analysis of circRNA-miRNA-mRNA ceRNA Network in Cardiac Hypertrophy |
| title_sort | integrated analysis of circrna mirna mrna cerna network in cardiac hypertrophy |
| topic | cardiac hypertrophy competitive endogenous RNA bioinformatics circRNA miRNA |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.781676/full |
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