circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3a
Muscle development is regulated under a series of complicate processes, and non-coding RNAs, such as microRNAs (miRNAs) and circular RNAs (circRNAs), have been reported to play important roles in regulating myoblast proliferation and differentiation. We found that miR-107 expression was high in skel...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-06-01
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| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253118300283 |
| _version_ | 1818134243626188800 |
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| author | Hui Li Xuefeng Wei Jiameng Yang Dong Dong Dan Hao Yongzhen Huang Xianyong Lan Martin Plath Chuzhao Lei Yun Ma Fengpeng Lin Yueyu Bai Hong Chen |
| author_facet | Hui Li Xuefeng Wei Jiameng Yang Dong Dong Dan Hao Yongzhen Huang Xianyong Lan Martin Plath Chuzhao Lei Yun Ma Fengpeng Lin Yueyu Bai Hong Chen |
| author_sort | Hui Li |
| collection | DOAJ |
| description | Muscle development is regulated under a series of complicate processes, and non-coding RNAs, such as microRNAs (miRNAs) and circular RNAs (circRNAs), have been reported to play important roles in regulating myoblast proliferation and differentiation. We found that miR-107 expression was high in skeletal muscle of Qinchuan cattle. Overexpression of miR-107 inhibited bovine myoblasts differentiation and protected cells from apoptosis. Wnt3a was identified as a target of miR-107 by luciferase activity, real-time qPCR, and western blotting assays. Knockdown of Wnt3a inhibited bovine myoblasts differentiation and apoptosis, and this effect was similar to miR-107 overexpression. We also found circFGFR4 to promote myoblasts differentiation and to induce cell apoptosis. Via luciferase screening and RNA pull-down assays, circFGFR4 was observed to sponge miR-107. Overexpression of circFGFR4 increased the expression of Wnt3a, whereas this effect was abolished by miR-107. These results demonstrated that circFGFR4 binding miR-107 promotes cell differentiation via targeting Wnt3a in bovine primary myoblasts. |
| first_indexed | 2024-12-11T09:05:31Z |
| format | Article |
| id | doaj.art-79c046d2ff794d7ca75f3402285e9a6e |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-11T09:05:31Z |
| publishDate | 2018-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-79c046d2ff794d7ca75f3402285e9a6e2022-12-22T01:13:39ZengElsevierMolecular Therapy: Nucleic Acids2162-25312018-06-0111C27228310.1016/j.omtn.2018.02.012circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3aHui Li0Xuefeng Wei1Jiameng Yang2Dong Dong3Dan Hao4Yongzhen Huang5Xianyong Lan6Martin Plath7Chuzhao Lei8Yun Ma9Fengpeng Lin10Yueyu Bai11Hong Chen12Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, ChinaCollege of Life Sciences, Xinyang Normal University, Institute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang, Henan 464000, PRCBureau of Animal Husbandry of Biyang County, Biyang, Henan 463700, ChinaAnimal Health Supervision in Henan Province, Zhengzhou, Henan 450008, ChinaShaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, ChinaMuscle development is regulated under a series of complicate processes, and non-coding RNAs, such as microRNAs (miRNAs) and circular RNAs (circRNAs), have been reported to play important roles in regulating myoblast proliferation and differentiation. We found that miR-107 expression was high in skeletal muscle of Qinchuan cattle. Overexpression of miR-107 inhibited bovine myoblasts differentiation and protected cells from apoptosis. Wnt3a was identified as a target of miR-107 by luciferase activity, real-time qPCR, and western blotting assays. Knockdown of Wnt3a inhibited bovine myoblasts differentiation and apoptosis, and this effect was similar to miR-107 overexpression. We also found circFGFR4 to promote myoblasts differentiation and to induce cell apoptosis. Via luciferase screening and RNA pull-down assays, circFGFR4 was observed to sponge miR-107. Overexpression of circFGFR4 increased the expression of Wnt3a, whereas this effect was abolished by miR-107. These results demonstrated that circFGFR4 binding miR-107 promotes cell differentiation via targeting Wnt3a in bovine primary myoblasts.http://www.sciencedirect.com/science/article/pii/S2162253118300283circRNAmiRNAWnt3amyoblastcattle |
| spellingShingle | Hui Li Xuefeng Wei Jiameng Yang Dong Dong Dan Hao Yongzhen Huang Xianyong Lan Martin Plath Chuzhao Lei Yun Ma Fengpeng Lin Yueyu Bai Hong Chen circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3a Molecular Therapy: Nucleic Acids circRNA miRNA Wnt3a myoblast cattle |
| title | circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3a |
| title_full | circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3a |
| title_fullStr | circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3a |
| title_full_unstemmed | circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3a |
| title_short | circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3a |
| title_sort | circfgfr4 promotes differentiation of myoblasts via binding mir 107 to relieve its inhibition of wnt3a |
| topic | circRNA miRNA Wnt3a myoblast cattle |
| url | http://www.sciencedirect.com/science/article/pii/S2162253118300283 |
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