Intrinsically Active and Pacemaker Neurons in Pluripotent Stem Cell-Derived Neuronal Populations
Neurons generated from pluripotent stem cells (PSCs) self-organize into functional neuronal assemblies in vitro, generating synchronous network activities. Intriguingly, PSC-derived neuronal assemblies develop spontaneous activities that are independent of external stimulation, suggesting the presen...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-03-01
|
| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221367111400023X |
| _version_ | 1828498512564715520 |
|---|---|
| author | Sebastian Illes Martin Jakab Felix Beyer Renate Gelfert Sébastien Couillard-Despres Alfons Schnitzler Markus Ritter Ludwig Aigner |
| author_facet | Sebastian Illes Martin Jakab Felix Beyer Renate Gelfert Sébastien Couillard-Despres Alfons Schnitzler Markus Ritter Ludwig Aigner |
| author_sort | Sebastian Illes |
| collection | DOAJ |
| description | Neurons generated from pluripotent stem cells (PSCs) self-organize into functional neuronal assemblies in vitro, generating synchronous network activities. Intriguingly, PSC-derived neuronal assemblies develop spontaneous activities that are independent of external stimulation, suggesting the presence of thus far undetected intrinsically active neurons (IANs). Here, by using mouse embryonic stem cells, we provide evidence for the existence of IANs in PSC-neuronal networks based on extracellular multielectrode array and intracellular patch-clamp recordings. IANs remain active after pharmacological inhibition of fast synaptic communication and possess intrinsic mechanisms required for autonomous neuronal activity. PSC-derived IANs are functionally integrated in PSC-neuronal populations, contribute to synchronous network bursting, and exhibit pacemaker properties. The intrinsic activity and pacemaker properties of the neuronal subpopulation identified herein may be particularly relevant for interventions involving transplantation of neural tissues. IANs may be a key element in the regulation of the functional activity of grafted as well as preexisting host neuronal networks. |
| first_indexed | 2024-12-11T13:08:33Z |
| format | Article |
| id | doaj.art-79c16dc1226148279d532ee63d657e19 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-11T13:08:33Z |
| publishDate | 2014-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-79c16dc1226148279d532ee63d657e192022-12-22T01:06:15ZengElsevierStem Cell Reports2213-67112014-03-012332333610.1016/j.stemcr.2014.01.006Intrinsically Active and Pacemaker Neurons in Pluripotent Stem Cell-Derived Neuronal PopulationsSebastian Illes0Martin Jakab1Felix Beyer2Renate Gelfert3Sébastien Couillard-Despres4Alfons Schnitzler5Markus Ritter6Ludwig Aigner7Institute of Molecular Regenerative Medicine, Paracelsus Medical University, 5020 Salzburg, Austria, AustriaInstitute of Physiology and Pathophysiology, Paracelsus Medical University, 5020 Salzburg, AustriaInstitute of Clinical Neuroscience and Medical Psychology, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, GermanyInstitute of Molecular Regenerative Medicine, Paracelsus Medical University, 5020 Salzburg, Austria, AustriaInstitute of Molecular Regenerative Medicine, Paracelsus Medical University, 5020 Salzburg, Austria, AustriaInstitute of Clinical Neuroscience and Medical Psychology, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, GermanyInstitute of Physiology and Pathophysiology, Paracelsus Medical University, 5020 Salzburg, AustriaInstitute of Molecular Regenerative Medicine, Paracelsus Medical University, 5020 Salzburg, Austria, AustriaNeurons generated from pluripotent stem cells (PSCs) self-organize into functional neuronal assemblies in vitro, generating synchronous network activities. Intriguingly, PSC-derived neuronal assemblies develop spontaneous activities that are independent of external stimulation, suggesting the presence of thus far undetected intrinsically active neurons (IANs). Here, by using mouse embryonic stem cells, we provide evidence for the existence of IANs in PSC-neuronal networks based on extracellular multielectrode array and intracellular patch-clamp recordings. IANs remain active after pharmacological inhibition of fast synaptic communication and possess intrinsic mechanisms required for autonomous neuronal activity. PSC-derived IANs are functionally integrated in PSC-neuronal populations, contribute to synchronous network bursting, and exhibit pacemaker properties. The intrinsic activity and pacemaker properties of the neuronal subpopulation identified herein may be particularly relevant for interventions involving transplantation of neural tissues. IANs may be a key element in the regulation of the functional activity of grafted as well as preexisting host neuronal networks.http://www.sciencedirect.com/science/article/pii/S221367111400023X |
| spellingShingle | Sebastian Illes Martin Jakab Felix Beyer Renate Gelfert Sébastien Couillard-Despres Alfons Schnitzler Markus Ritter Ludwig Aigner Intrinsically Active and Pacemaker Neurons in Pluripotent Stem Cell-Derived Neuronal Populations Stem Cell Reports |
| title | Intrinsically Active and Pacemaker Neurons in Pluripotent Stem Cell-Derived Neuronal Populations |
| title_full | Intrinsically Active and Pacemaker Neurons in Pluripotent Stem Cell-Derived Neuronal Populations |
| title_fullStr | Intrinsically Active and Pacemaker Neurons in Pluripotent Stem Cell-Derived Neuronal Populations |
| title_full_unstemmed | Intrinsically Active and Pacemaker Neurons in Pluripotent Stem Cell-Derived Neuronal Populations |
| title_short | Intrinsically Active and Pacemaker Neurons in Pluripotent Stem Cell-Derived Neuronal Populations |
| title_sort | intrinsically active and pacemaker neurons in pluripotent stem cell derived neuronal populations |
| url | http://www.sciencedirect.com/science/article/pii/S221367111400023X |
| work_keys_str_mv | AT sebastianilles intrinsicallyactiveandpacemakerneuronsinpluripotentstemcellderivedneuronalpopulations AT martinjakab intrinsicallyactiveandpacemakerneuronsinpluripotentstemcellderivedneuronalpopulations AT felixbeyer intrinsicallyactiveandpacemakerneuronsinpluripotentstemcellderivedneuronalpopulations AT renategelfert intrinsicallyactiveandpacemakerneuronsinpluripotentstemcellderivedneuronalpopulations AT sebastiencouillarddespres intrinsicallyactiveandpacemakerneuronsinpluripotentstemcellderivedneuronalpopulations AT alfonsschnitzler intrinsicallyactiveandpacemakerneuronsinpluripotentstemcellderivedneuronalpopulations AT markusritter intrinsicallyactiveandpacemakerneuronsinpluripotentstemcellderivedneuronalpopulations AT ludwigaigner intrinsicallyactiveandpacemakerneuronsinpluripotentstemcellderivedneuronalpopulations |