GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia
Summary: The neural inhibitory gamma-aminobutyric acid (GABA) system in the regulation of anesthetic consciousness is heterogeneous, and the medial hypothalamus (MH), consisting of ventromedial hypothalamus (VMH) and dorsomedial hypothalamus (DMH), plays an important role in sleep and circadian rhyt...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222021861 |
| _version_ | 1797946167014719488 |
|---|---|
| author | Yanfeng Wang Yanping Song Li Tong Lu Wang Jiangbei Cao Gang Qin Xingyang Liu Weidong Mi E. Wang Yongxin Guo |
| author_facet | Yanfeng Wang Yanping Song Li Tong Lu Wang Jiangbei Cao Gang Qin Xingyang Liu Weidong Mi E. Wang Yongxin Guo |
| author_sort | Yanfeng Wang |
| collection | DOAJ |
| description | Summary: The neural inhibitory gamma-aminobutyric acid (GABA) system in the regulation of anesthetic consciousness is heterogeneous, and the medial hypothalamus (MH), consisting of ventromedial hypothalamus (VMH) and dorsomedial hypothalamus (DMH), plays an important role in sleep and circadian rhythm. However, the role of MH GABAergic neurons (MHGABA) in anesthesia remains unclear. In this study, we used righting reflex, electroencephalogram (EEG), and arousal behavioral score to evaluate the sevoflurane anesthesia. Activation of MHGABA or DMHGABA neurons prolonged the anesthesia induction time, shortened the anesthesia emergence time, and induced EEG arousal and body movement during anesthesia; meanwhile, VMHGABA neurons activated only induced EEG changes during 1.5% sevoflurane anesthesia. Furthermore, inhibition of DMHGABA neurons significantly deepened sevoflurane anesthesia. Therefore, DMHGABA neurons exert a strong emergence-promoting effect on induction, maintenance, and arousal during sevoflurane general anesthesia, which helps to reveal the mechanism of anesthesia. |
| first_indexed | 2024-04-10T21:06:36Z |
| format | Article |
| id | doaj.art-79c810a9d956431aa0ea22fde1ae4994 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T21:06:36Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-79c810a9d956431aa0ea22fde1ae49942023-01-22T04:41:51ZengElsevieriScience2589-00422023-01-01261105913GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesiaYanfeng Wang0Yanping Song1Li Tong2Lu Wang3Jiangbei Cao4Gang Qin5Xingyang Liu6Weidong Mi7E. Wang8Yongxin Guo9Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, ChinaDepartment of Anesthesia, 922 Hospital of PLA, Hengyang, Hunan 421002, ChinaAnesthesia and Operation Centre, the First Medical Centre of Chinese PLA General Hospital, Beijing 100853, ChinaDepartment of Anesthesiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, ChinaAnesthesia and Operation Centre, the First Medical Centre of Chinese PLA General Hospital, Beijing 100853, ChinaDepartment of Anesthesiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, ChinaDepartment of Anesthesiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, ChinaAnesthesia and Operation Centre, the First Medical Centre of Chinese PLA General Hospital, Beijing 100853, ChinaDepartment of Anesthesiology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Corresponding authorAnesthesia and Operation Centre, the First Medical Centre of Chinese PLA General Hospital, Beijing 100853, China; Corresponding authorSummary: The neural inhibitory gamma-aminobutyric acid (GABA) system in the regulation of anesthetic consciousness is heterogeneous, and the medial hypothalamus (MH), consisting of ventromedial hypothalamus (VMH) and dorsomedial hypothalamus (DMH), plays an important role in sleep and circadian rhythm. However, the role of MH GABAergic neurons (MHGABA) in anesthesia remains unclear. In this study, we used righting reflex, electroencephalogram (EEG), and arousal behavioral score to evaluate the sevoflurane anesthesia. Activation of MHGABA or DMHGABA neurons prolonged the anesthesia induction time, shortened the anesthesia emergence time, and induced EEG arousal and body movement during anesthesia; meanwhile, VMHGABA neurons activated only induced EEG changes during 1.5% sevoflurane anesthesia. Furthermore, inhibition of DMHGABA neurons significantly deepened sevoflurane anesthesia. Therefore, DMHGABA neurons exert a strong emergence-promoting effect on induction, maintenance, and arousal during sevoflurane general anesthesia, which helps to reveal the mechanism of anesthesia.http://www.sciencedirect.com/science/article/pii/S2589004222021861Biological sciencesNeuroscienceSystems neuroscienceCellular neuroscience |
| spellingShingle | Yanfeng Wang Yanping Song Li Tong Lu Wang Jiangbei Cao Gang Qin Xingyang Liu Weidong Mi E. Wang Yongxin Guo GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia iScience Biological sciences Neuroscience Systems neuroscience Cellular neuroscience |
| title | GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia |
| title_full | GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia |
| title_fullStr | GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia |
| title_full_unstemmed | GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia |
| title_short | GABAergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia |
| title_sort | gabaergic neurons in the dorsomedial hypothalamus regulate states of consciousness in sevoflurane anesthesia |
| topic | Biological sciences Neuroscience Systems neuroscience Cellular neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222021861 |
| work_keys_str_mv | AT yanfengwang gabaergicneuronsinthedorsomedialhypothalamusregulatestatesofconsciousnessinsevofluraneanesthesia AT yanpingsong gabaergicneuronsinthedorsomedialhypothalamusregulatestatesofconsciousnessinsevofluraneanesthesia AT litong gabaergicneuronsinthedorsomedialhypothalamusregulatestatesofconsciousnessinsevofluraneanesthesia AT luwang gabaergicneuronsinthedorsomedialhypothalamusregulatestatesofconsciousnessinsevofluraneanesthesia AT jiangbeicao gabaergicneuronsinthedorsomedialhypothalamusregulatestatesofconsciousnessinsevofluraneanesthesia AT gangqin gabaergicneuronsinthedorsomedialhypothalamusregulatestatesofconsciousnessinsevofluraneanesthesia AT xingyangliu gabaergicneuronsinthedorsomedialhypothalamusregulatestatesofconsciousnessinsevofluraneanesthesia AT weidongmi gabaergicneuronsinthedorsomedialhypothalamusregulatestatesofconsciousnessinsevofluraneanesthesia AT ewang gabaergicneuronsinthedorsomedialhypothalamusregulatestatesofconsciousnessinsevofluraneanesthesia AT yongxinguo gabaergicneuronsinthedorsomedialhypothalamusregulatestatesofconsciousnessinsevofluraneanesthesia |