Foot-and-mouth disease virus O/ME-SA/Ind 2001 lineage outbreak in vaccinated Holstein Friesian cattle in Saudi Arabia in 2016
Background: Foot-and-mouth disease virus (FMDV) is a highly contagious viral infection of large ruminants. Despite the massive application of vaccines against FMDV, several outbreaks are still being reported in Africa and Asia. Aim: To perform molecular characterization of FMDV in an outbreak among...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Veterinary Quarterly |
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| Online Access: | http://dx.doi.org/10.1080/01652176.2018.1539568 |
| _version_ | 1818331925287272448 |
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| author | Maged Gomaa Hemida Walid Rizk EL-Ghareeb Fahad Al-Hizab Abdelazim Ibrahim |
| author_facet | Maged Gomaa Hemida Walid Rizk EL-Ghareeb Fahad Al-Hizab Abdelazim Ibrahim |
| author_sort | Maged Gomaa Hemida |
| collection | DOAJ |
| description | Background: Foot-and-mouth disease virus (FMDV) is a highly contagious viral infection of large ruminants. Despite the massive application of vaccines against FMDV, several outbreaks are still being reported in Africa and Asia. Aim: To perform molecular characterization of FMDV in an outbreak among a cattle herd Saudi Arabia in 2016. This herd had been vaccinated with a polyvalent FMDV vaccine. Methods: To investigate this outbreak, we collected specimens from 77 animals showing typical clinical signs of FMDV. Specimens including sera, nasal swabs, and tissues (tongue, coronary bands, hooves, and hearts) were collected. We tested the collected cattle sera for the presence of FMDV antibodies with commercial ELISA kits. In addition, we tested the swabs for the presence of the most common FMDV strains (O, A, Asia-1 and SAT-2) with RT-PCR using serotype-specific oligonucleotides. Results: Serology showed that 22% of the tested sera were positive. Molecular testing of the examined swabs confirmed that 24% of the tested animals were positive. Our sequencing analysis confirmed that the circulating strains of FMDV belonged to FMDV serotype O. The phylogenetic tree based on the FMDV-VP-1 gene revealed high nucleotide identity between the circulating strains and the Bangladesh strain (99%). These strains were distinct (shared 89% nucleotide identity) from the FMDV-O strains used for the preparation of the vaccine administered to the animals in this herd. Moreover, they had 7% nucleotide difference between the FMDV-O strains reported in Saudi Arabian in 2013. Conclusion: More in-depth molecular characterization of these FMDV strains is warranted. |
| first_indexed | 2024-12-13T13:27:35Z |
| format | Article |
| id | doaj.art-79cb137333cb4693955816f2c6c710f0 |
| institution | Directory Open Access Journal |
| issn | 0165-2176 1875-5941 |
| language | English |
| last_indexed | 2024-12-13T13:27:35Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Veterinary Quarterly |
| spelling | doaj.art-79cb137333cb4693955816f2c6c710f02022-12-21T23:44:16ZengTaylor & Francis GroupVeterinary Quarterly0165-21761875-59412018-01-01381889810.1080/01652176.2018.15395681539568Foot-and-mouth disease virus O/ME-SA/Ind 2001 lineage outbreak in vaccinated Holstein Friesian cattle in Saudi Arabia in 2016Maged Gomaa Hemida0Walid Rizk EL-Ghareeb1Fahad Al-Hizab2Abdelazim Ibrahim3King Faisal UniversityKing Faisal UniversityKing Faisal UniversityKing Faisal UniversityBackground: Foot-and-mouth disease virus (FMDV) is a highly contagious viral infection of large ruminants. Despite the massive application of vaccines against FMDV, several outbreaks are still being reported in Africa and Asia. Aim: To perform molecular characterization of FMDV in an outbreak among a cattle herd Saudi Arabia in 2016. This herd had been vaccinated with a polyvalent FMDV vaccine. Methods: To investigate this outbreak, we collected specimens from 77 animals showing typical clinical signs of FMDV. Specimens including sera, nasal swabs, and tissues (tongue, coronary bands, hooves, and hearts) were collected. We tested the collected cattle sera for the presence of FMDV antibodies with commercial ELISA kits. In addition, we tested the swabs for the presence of the most common FMDV strains (O, A, Asia-1 and SAT-2) with RT-PCR using serotype-specific oligonucleotides. Results: Serology showed that 22% of the tested sera were positive. Molecular testing of the examined swabs confirmed that 24% of the tested animals were positive. Our sequencing analysis confirmed that the circulating strains of FMDV belonged to FMDV serotype O. The phylogenetic tree based on the FMDV-VP-1 gene revealed high nucleotide identity between the circulating strains and the Bangladesh strain (99%). These strains were distinct (shared 89% nucleotide identity) from the FMDV-O strains used for the preparation of the vaccine administered to the animals in this herd. Moreover, they had 7% nucleotide difference between the FMDV-O strains reported in Saudi Arabian in 2013. Conclusion: More in-depth molecular characterization of these FMDV strains is warranted.http://dx.doi.org/10.1080/01652176.2018.1539568cattlefoot-and-mouth diseasepcrpathologyserotype osaudi arabia |
| spellingShingle | Maged Gomaa Hemida Walid Rizk EL-Ghareeb Fahad Al-Hizab Abdelazim Ibrahim Foot-and-mouth disease virus O/ME-SA/Ind 2001 lineage outbreak in vaccinated Holstein Friesian cattle in Saudi Arabia in 2016 Veterinary Quarterly cattle foot-and-mouth disease pcr pathology serotype o saudi arabia |
| title | Foot-and-mouth disease virus O/ME-SA/Ind 2001 lineage outbreak in vaccinated Holstein Friesian cattle in Saudi Arabia in 2016 |
| title_full | Foot-and-mouth disease virus O/ME-SA/Ind 2001 lineage outbreak in vaccinated Holstein Friesian cattle in Saudi Arabia in 2016 |
| title_fullStr | Foot-and-mouth disease virus O/ME-SA/Ind 2001 lineage outbreak in vaccinated Holstein Friesian cattle in Saudi Arabia in 2016 |
| title_full_unstemmed | Foot-and-mouth disease virus O/ME-SA/Ind 2001 lineage outbreak in vaccinated Holstein Friesian cattle in Saudi Arabia in 2016 |
| title_short | Foot-and-mouth disease virus O/ME-SA/Ind 2001 lineage outbreak in vaccinated Holstein Friesian cattle in Saudi Arabia in 2016 |
| title_sort | foot and mouth disease virus o me sa ind 2001 lineage outbreak in vaccinated holstein friesian cattle in saudi arabia in 2016 |
| topic | cattle foot-and-mouth disease pcr pathology serotype o saudi arabia |
| url | http://dx.doi.org/10.1080/01652176.2018.1539568 |
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