Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae
In Saccharomyces cerevisiae, the mitochondrial carrier family protein Pic2 imports copper into the matrix. Deletion of PIC2 causes defects in mitochondrial copper uptake and copper-dependent growth phenotypes owing to decreased cytochrome c oxidase activity. However, copper import is not completely...
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| Format: | Article |
| Language: | English |
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The Royal Society
2016-01-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150223 |
| _version_ | 1818260487820804096 |
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| author | Katherine E. Vest Jing Wang Micah G. Gammon Margaret K. Maynard Olivia L. White Jai A. Cobine Wilkerson K. Mahone Paul A. Cobine |
| author_facet | Katherine E. Vest Jing Wang Micah G. Gammon Margaret K. Maynard Olivia L. White Jai A. Cobine Wilkerson K. Mahone Paul A. Cobine |
| author_sort | Katherine E. Vest |
| collection | DOAJ |
| description | In Saccharomyces cerevisiae, the mitochondrial carrier family protein Pic2 imports copper into the matrix. Deletion of PIC2 causes defects in mitochondrial copper uptake and copper-dependent growth phenotypes owing to decreased cytochrome c oxidase activity. However, copper import is not completely eliminated in this mutant, so alternative transport systems must exist. Deletion of MRS3, a component of the iron import machinery, also causes a copper-dependent growth defect on non-fermentable carbon. Deletion of both PIC2 and MRS3 led to a more severe respiratory growth defect than either individual mutant. In addition, MRS3 expressed from a high copy number vector was able to suppress the oxygen consumption and copper uptake defects of a strain lacking PIC2. When expressed in Lactococcus lactis, Mrs3 mediated copper and iron import. Finally, a PIC2 and MRS3 double mutant prevented the copper-dependent activation of a heterologously expressed copper sensor in the mitochondrial intermembrane space. Taken together, these data support a role for the iron transporter Mrs3 in copper import into the mitochondrial matrix. |
| first_indexed | 2024-12-12T18:32:07Z |
| format | Article |
| id | doaj.art-79ccb51be14a4ab58ed0058f903efc3d |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-12-12T18:32:07Z |
| publishDate | 2016-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-79ccb51be14a4ab58ed0058f903efc3d2022-12-22T00:15:55ZengThe Royal SocietyOpen Biology2046-24412016-01-016110.1098/rsob.150223150223Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiaeKatherine E. VestJing WangMicah G. GammonMargaret K. MaynardOlivia L. WhiteJai A. CobineWilkerson K. MahonePaul A. CobineIn Saccharomyces cerevisiae, the mitochondrial carrier family protein Pic2 imports copper into the matrix. Deletion of PIC2 causes defects in mitochondrial copper uptake and copper-dependent growth phenotypes owing to decreased cytochrome c oxidase activity. However, copper import is not completely eliminated in this mutant, so alternative transport systems must exist. Deletion of MRS3, a component of the iron import machinery, also causes a copper-dependent growth defect on non-fermentable carbon. Deletion of both PIC2 and MRS3 led to a more severe respiratory growth defect than either individual mutant. In addition, MRS3 expressed from a high copy number vector was able to suppress the oxygen consumption and copper uptake defects of a strain lacking PIC2. When expressed in Lactococcus lactis, Mrs3 mediated copper and iron import. Finally, a PIC2 and MRS3 double mutant prevented the copper-dependent activation of a heterologously expressed copper sensor in the mitochondrial intermembrane space. Taken together, these data support a role for the iron transporter Mrs3 in copper import into the mitochondrial matrix.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150223copperironcytochrome c oxidasemitochondrial carrier family |
| spellingShingle | Katherine E. Vest Jing Wang Micah G. Gammon Margaret K. Maynard Olivia L. White Jai A. Cobine Wilkerson K. Mahone Paul A. Cobine Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae Open Biology copper iron cytochrome c oxidase mitochondrial carrier family |
| title | Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae |
| title_full | Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae |
| title_fullStr | Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae |
| title_full_unstemmed | Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae |
| title_short | Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae |
| title_sort | overlap of copper and iron uptake systems in mitochondria in saccharomyces cerevisiae |
| topic | copper iron cytochrome c oxidase mitochondrial carrier family |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150223 |
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