Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19
Current point-of-care (POC) screening of Coronavirus disease 2019 (COVID-19) requires further improvements to achieve highly sensitive, rapid, and inexpensive detection. Here we describe an immunoresistive sensor on a polyethylene terephthalate (PET) film for simple, inexpensive, and highly sensitiv...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/3/149 |
| _version_ | 1797446971577860096 |
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| author | Tianyi Li Scott D. Soelberg Zachary Taylor Vigneshwar Sakthivelpathi Clement E. Furlong Jong-Hoon Kim Sang-gyeun Ahn Peter D. Han Lea M. Starita Jia Zhu Jae-Hyun Chung |
| author_facet | Tianyi Li Scott D. Soelberg Zachary Taylor Vigneshwar Sakthivelpathi Clement E. Furlong Jong-Hoon Kim Sang-gyeun Ahn Peter D. Han Lea M. Starita Jia Zhu Jae-Hyun Chung |
| author_sort | Tianyi Li |
| collection | DOAJ |
| description | Current point-of-care (POC) screening of Coronavirus disease 2019 (COVID-19) requires further improvements to achieve highly sensitive, rapid, and inexpensive detection. Here we describe an immunoresistive sensor on a polyethylene terephthalate (PET) film for simple, inexpensive, and highly sensitive COVID-19 screening. The sensor is composed of single-walled carbon nanotubes (SWCNTs) functionalized with monoclonal antibodies that bind to the spike protein of SARS-CoV-2. Silver electrodes are silkscreen-printed on SWCNTs to reduce contact resistance. We determine the SARS-CoV-2 status via the resistance ratio of control- and SARS-CoV-2 sensor electrodes. A combined measurement of two adjacent sensors enhances the sensitivity and specificity of the detection protocol. The lower limit of detection (LLD) of the SWCNT assay is 350 genome equivalents/mL. The developed SWCNT sensor shows 100% sensitivity and 90% specificity in clinical sample testing. Further, our device adds benefits of a small form factor, simple operation, low power requirement, and low assay cost. This highly sensitive film sensor will facilitate rapid COVID-19 screening and expedite the development of POC screening platforms. |
| first_indexed | 2024-03-09T13:49:11Z |
| format | Article |
| id | doaj.art-79cd3dac4d0f49ce93e0549d999d92bb |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T13:49:11Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-79cd3dac4d0f49ce93e0549d999d92bb2023-11-30T20:53:57ZengMDPI AGBiosensors2079-63742022-02-0112314910.3390/bios12030149Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19Tianyi Li0Scott D. Soelberg1Zachary Taylor2Vigneshwar Sakthivelpathi3Clement E. Furlong4Jong-Hoon Kim5Sang-gyeun Ahn6Peter D. Han7Lea M. Starita8Jia Zhu9Jae-Hyun Chung10Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USADepartments of Medicine, Division of Medical Genetics and Genome Sciences, University of Washington, Seattle, WA 98195, USADepartment of Mechanical Engineering, University of Washington, Seattle, WA 98195, USADepartment of Mechanical Engineering, University of Washington, Seattle, WA 98195, USADepartments of Medicine, Division of Medical Genetics and Genome Sciences, University of Washington, Seattle, WA 98195, USASchool of Engineering and Computer Science, Washington State University, Vancouver, WA 98686, USAIndustrial Design, University of Washington, Seattle, WA 98195, USADepartment of Genome Sciences, University of Washington, Seattle, WA 98195, USADepartment of Genome Sciences, University of Washington, Seattle, WA 98195, USADepartment of Laboratory Medicine and Pathology, University of Washington, and Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USADepartment of Mechanical Engineering, University of Washington, Seattle, WA 98195, USACurrent point-of-care (POC) screening of Coronavirus disease 2019 (COVID-19) requires further improvements to achieve highly sensitive, rapid, and inexpensive detection. Here we describe an immunoresistive sensor on a polyethylene terephthalate (PET) film for simple, inexpensive, and highly sensitive COVID-19 screening. The sensor is composed of single-walled carbon nanotubes (SWCNTs) functionalized with monoclonal antibodies that bind to the spike protein of SARS-CoV-2. Silver electrodes are silkscreen-printed on SWCNTs to reduce contact resistance. We determine the SARS-CoV-2 status via the resistance ratio of control- and SARS-CoV-2 sensor electrodes. A combined measurement of two adjacent sensors enhances the sensitivity and specificity of the detection protocol. The lower limit of detection (LLD) of the SWCNT assay is 350 genome equivalents/mL. The developed SWCNT sensor shows 100% sensitivity and 90% specificity in clinical sample testing. Further, our device adds benefits of a small form factor, simple operation, low power requirement, and low assay cost. This highly sensitive film sensor will facilitate rapid COVID-19 screening and expedite the development of POC screening platforms.https://www.mdpi.com/2079-6374/12/3/149single-walled carbon nanotubesimmunoresistive sensorresistance ratioCOVID-19point-of-care diagnosis |
| spellingShingle | Tianyi Li Scott D. Soelberg Zachary Taylor Vigneshwar Sakthivelpathi Clement E. Furlong Jong-Hoon Kim Sang-gyeun Ahn Peter D. Han Lea M. Starita Jia Zhu Jae-Hyun Chung Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19 Biosensors single-walled carbon nanotubes immunoresistive sensor resistance ratio COVID-19 point-of-care diagnosis |
| title | Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19 |
| title_full | Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19 |
| title_fullStr | Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19 |
| title_full_unstemmed | Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19 |
| title_short | Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19 |
| title_sort | highly sensitive immunoresistive sensor for point of care screening for covid 19 |
| topic | single-walled carbon nanotubes immunoresistive sensor resistance ratio COVID-19 point-of-care diagnosis |
| url | https://www.mdpi.com/2079-6374/12/3/149 |
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