Crystallographic and Physicochemical Analysis of Bovine and Human Teeth Using X-ray Diffraction and Solid-State Nuclear Magnetic Resonance
Dental research often uses bovine teeth as a substitute for human teeth. The aim of this study was to evaluate differences in the crystalline nanostructures of enamel and dentin between bovine and human teeth, using X-ray diffraction (XRD) and solid-state nuclear magnetic resonance (NMR). The crysta...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/4/254 |
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| author | Noriko Hiraishi Tadamu Gondo Yasushi Shimada Robert Hill Fumiaki Hayashi |
| author_facet | Noriko Hiraishi Tadamu Gondo Yasushi Shimada Robert Hill Fumiaki Hayashi |
| author_sort | Noriko Hiraishi |
| collection | DOAJ |
| description | Dental research often uses bovine teeth as a substitute for human teeth. The aim of this study was to evaluate differences in the crystalline nanostructures of enamel and dentin between bovine and human teeth, using X-ray diffraction (XRD) and solid-state nuclear magnetic resonance (NMR). The crystallite size (crystallinity) and microstrains were analyzed using XRD with the Rietveld refinement technique and the Halder–Wagner method. The <sup>31</sup>P and <sup>1</sup>H NMR chemical environments were analyzed by two-dimensional (2D) <sup>1</sup>H-<sup>31</sup>P heteronuclear-correlation (HETCOR) magic-angle spinning (MAS) NMR spectroscopy. Enamel had a greater crystallite size and fewer microstrains than dentin for both bovine and human teeth. When compared between the species, the bovine apatite had a smaller crystallite size with more microstrains than the human apatite for both dentin and enamel. The 2D HETCOR spectra demonstrated that a water-rich layer and inorganic HPO<sub>4</sub><sup>2−</sup> ions were abundant in dentin; meanwhile, the hydroxyl group in the lattice site was more dominant in enamel. A greater intensity of the hydroxyl group was detected in human than in bovine for both dentin and enamel. For <sup>31</sup>P projections, bovine dentin and bovine enamel have wider linewidths than human dentin and human enamel, respectively. There are differences in the crystallite profile between human and bovine. The results of dental research should be interpreted with caution when bovine teeth are substituted for human teeth. |
| first_indexed | 2024-03-09T16:15:53Z |
| format | Article |
| id | doaj.art-79d00877ad024868ad8ca01eb3b6d7f9 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T16:15:53Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-79d00877ad024868ad8ca01eb3b6d7f92023-11-24T15:50:19ZengMDPI AGJournal of Functional Biomaterials2079-49832022-11-0113425410.3390/jfb13040254Crystallographic and Physicochemical Analysis of Bovine and Human Teeth Using X-ray Diffraction and Solid-State Nuclear Magnetic ResonanceNoriko Hiraishi0Tadamu Gondo1Yasushi Shimada2Robert Hill3Fumiaki Hayashi4Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, JapanDepartment of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, JapanDepartment of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8549, JapanDental Physical Sciences Unit, Institute of Dentistry, Queen Mary University of London, London E1 4NS, UKNMR Operation Team, Laboratory for Advanced NMR Application and Development, RIKEN Center for Biosystems Dynamics Research, Yokohama 230-0045, JapanDental research often uses bovine teeth as a substitute for human teeth. The aim of this study was to evaluate differences in the crystalline nanostructures of enamel and dentin between bovine and human teeth, using X-ray diffraction (XRD) and solid-state nuclear magnetic resonance (NMR). The crystallite size (crystallinity) and microstrains were analyzed using XRD with the Rietveld refinement technique and the Halder–Wagner method. The <sup>31</sup>P and <sup>1</sup>H NMR chemical environments were analyzed by two-dimensional (2D) <sup>1</sup>H-<sup>31</sup>P heteronuclear-correlation (HETCOR) magic-angle spinning (MAS) NMR spectroscopy. Enamel had a greater crystallite size and fewer microstrains than dentin for both bovine and human teeth. When compared between the species, the bovine apatite had a smaller crystallite size with more microstrains than the human apatite for both dentin and enamel. The 2D HETCOR spectra demonstrated that a water-rich layer and inorganic HPO<sub>4</sub><sup>2−</sup> ions were abundant in dentin; meanwhile, the hydroxyl group in the lattice site was more dominant in enamel. A greater intensity of the hydroxyl group was detected in human than in bovine for both dentin and enamel. For <sup>31</sup>P projections, bovine dentin and bovine enamel have wider linewidths than human dentin and human enamel, respectively. There are differences in the crystallite profile between human and bovine. The results of dental research should be interpreted with caution when bovine teeth are substituted for human teeth.https://www.mdpi.com/2079-4983/13/4/254enameldentinapatitesX-ray diffractionsolid-state nuclear magnetic resonanceheteronuclear |
| spellingShingle | Noriko Hiraishi Tadamu Gondo Yasushi Shimada Robert Hill Fumiaki Hayashi Crystallographic and Physicochemical Analysis of Bovine and Human Teeth Using X-ray Diffraction and Solid-State Nuclear Magnetic Resonance Journal of Functional Biomaterials enamel dentin apatites X-ray diffraction solid-state nuclear magnetic resonance heteronuclear |
| title | Crystallographic and Physicochemical Analysis of Bovine and Human Teeth Using X-ray Diffraction and Solid-State Nuclear Magnetic Resonance |
| title_full | Crystallographic and Physicochemical Analysis of Bovine and Human Teeth Using X-ray Diffraction and Solid-State Nuclear Magnetic Resonance |
| title_fullStr | Crystallographic and Physicochemical Analysis of Bovine and Human Teeth Using X-ray Diffraction and Solid-State Nuclear Magnetic Resonance |
| title_full_unstemmed | Crystallographic and Physicochemical Analysis of Bovine and Human Teeth Using X-ray Diffraction and Solid-State Nuclear Magnetic Resonance |
| title_short | Crystallographic and Physicochemical Analysis of Bovine and Human Teeth Using X-ray Diffraction and Solid-State Nuclear Magnetic Resonance |
| title_sort | crystallographic and physicochemical analysis of bovine and human teeth using x ray diffraction and solid state nuclear magnetic resonance |
| topic | enamel dentin apatites X-ray diffraction solid-state nuclear magnetic resonance heteronuclear |
| url | https://www.mdpi.com/2079-4983/13/4/254 |
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