Knockdown of <i>p</i>-Coumaroyl Shikimate/Quinate 3′-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage Roots
Cassava storage roots are an important source of food, feed, and material for starch-based industries in many countries. After harvest, rapid post-harvest physiological deterioration (PPD) reduces their palatability and marketability. During the PPD process, vascular streaking occurs through over-ac...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9231 |
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| author | Qiuxiang Ma Jia Xu Yancai Feng Xiaoyun Wu Xinlu Lu Peng Zhang |
| author_facet | Qiuxiang Ma Jia Xu Yancai Feng Xiaoyun Wu Xinlu Lu Peng Zhang |
| author_sort | Qiuxiang Ma |
| collection | DOAJ |
| description | Cassava storage roots are an important source of food, feed, and material for starch-based industries in many countries. After harvest, rapid post-harvest physiological deterioration (PPD) reduces their palatability and marketability. During the PPD process, vascular streaking occurs through over-accumulation of coumarins, the biosynthesis of which involves the key enzyme <i>p</i>-coumaroyl shikimate/quinate 3′-hydroxylase (C3′H). Repression of <i>MeC3′H</i> expression by RNA interference in transgenic cassava plants caused a significant delay in PPD by decreasing scopoletin and scopolin accumulation in field-harvested storage roots. This study demonstrates that MeC3′H is the key enzyme participating in coumarin biosynthesis during PPD and shows that <i>MeC3′H</i> is a useful target gene for editing to prolong the shelf life of cassava storage roots. |
| first_indexed | 2024-03-09T09:55:26Z |
| format | Article |
| id | doaj.art-79d3ab0e3c0e4e9e907a8898dd4caa87 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:55:26Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-79d3ab0e3c0e4e9e907a8898dd4caa872023-12-01T23:47:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316923110.3390/ijms23169231Knockdown of <i>p</i>-Coumaroyl Shikimate/Quinate 3′-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage RootsQiuxiang Ma0Jia Xu1Yancai Feng2Xiaoyun Wu3Xinlu Lu4Peng Zhang5National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, ChinaNational Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, ChinaNational Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, ChinaNational Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, ChinaNational Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, ChinaNational Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, ChinaCassava storage roots are an important source of food, feed, and material for starch-based industries in many countries. After harvest, rapid post-harvest physiological deterioration (PPD) reduces their palatability and marketability. During the PPD process, vascular streaking occurs through over-accumulation of coumarins, the biosynthesis of which involves the key enzyme <i>p</i>-coumaroyl shikimate/quinate 3′-hydroxylase (C3′H). Repression of <i>MeC3′H</i> expression by RNA interference in transgenic cassava plants caused a significant delay in PPD by decreasing scopoletin and scopolin accumulation in field-harvested storage roots. This study demonstrates that MeC3′H is the key enzyme participating in coumarin biosynthesis during PPD and shows that <i>MeC3′H</i> is a useful target gene for editing to prolong the shelf life of cassava storage roots.https://www.mdpi.com/1422-0067/23/16/9231cassavaPPDC3′Hscopoletinscopolin |
| spellingShingle | Qiuxiang Ma Jia Xu Yancai Feng Xiaoyun Wu Xinlu Lu Peng Zhang Knockdown of <i>p</i>-Coumaroyl Shikimate/Quinate 3′-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage Roots International Journal of Molecular Sciences cassava PPD C3′H scopoletin scopolin |
| title | Knockdown of <i>p</i>-Coumaroyl Shikimate/Quinate 3′-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage Roots |
| title_full | Knockdown of <i>p</i>-Coumaroyl Shikimate/Quinate 3′-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage Roots |
| title_fullStr | Knockdown of <i>p</i>-Coumaroyl Shikimate/Quinate 3′-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage Roots |
| title_full_unstemmed | Knockdown of <i>p</i>-Coumaroyl Shikimate/Quinate 3′-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage Roots |
| title_short | Knockdown of <i>p</i>-Coumaroyl Shikimate/Quinate 3′-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage Roots |
| title_sort | knockdown of i p i coumaroyl shikimate quinate 3 hydroxylase delays the occurrence of post harvest physiological deterioration in cassava storage roots |
| topic | cassava PPD C3′H scopoletin scopolin |
| url | https://www.mdpi.com/1422-0067/23/16/9231 |
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