Genetic and Physical Mapping of the RPP13 Locus, in Arabidopsis, Responsible for Specific Recognition of Several Peronospora parasitica (Downy Mildew) Isolates
Fifteen isolates of the biotrophic oomycete Peronospora parasitica (downy mildew) were obtained from a population of Arabidopsis thaliana plants that established naturally in a garden the previous year. They exhibited phenotypic variation in a set of 12 Arabidopsis accessions that suggested that the...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1999-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.9.792 |
| _version_ | 1811291209157574656 |
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| author | Peter Bittner-Eddy Canan Can Nick Gunn Matthieu Pinel Mahmut Tör Ian Crute Eric B. Holub Jim Beynon |
| author_facet | Peter Bittner-Eddy Canan Can Nick Gunn Matthieu Pinel Mahmut Tör Ian Crute Eric B. Holub Jim Beynon |
| author_sort | Peter Bittner-Eddy |
| collection | DOAJ |
| description | Fifteen isolates of the biotrophic oomycete Peronospora parasitica (downy mildew) were obtained from a population of Arabidopsis thaliana plants that established naturally in a garden the previous year. They exhibited phenotypic variation in a set of 12 Arabidopsis accessions that suggested that the parasite population consisted of at least six pathotypes. One isolate, Maks9, elicited an interaction phenotype of flecking necrosis and no sporulation (FN) in the Arabidopsis accession Nd-1, and more extensive pitting necrosis with no sporulation (PN) in the accession Ws-2. RPP13 was designated as the locus for a single dominant resistance gene associated with the resistance in Nd-1 and mapped to an interval of approximately 60 kb on a bacterial artificial chromosome (BAC) contig on the lower arm of chromosome 3. This locus is approximately 6 cM telomeric to RPP1, which was previously described as the locus for the PN interaction with five Peronospora isolates, including resistance to Maks9 in Ws-2. New Peronospora isolates were obtained from four other geographically distinct populations of P. parasitica. Four isolates were characterized that elicited an FN phenotype in Nd-1 and mapped resistance to the RPP13 locus. This suggests that the RPP13 locus contains either a single gene capable of multiple isolate recognition or a group of tightly linked genes. Further analysis suggests that the RPP11 gene in the accession Rld-0 may be allelic to RPP13 but results in a different recognition capability. |
| first_indexed | 2024-04-13T04:25:05Z |
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| id | doaj.art-79d3bcd557144acca3d414164f7d6d88 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:25:05Z |
| publishDate | 1999-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-79d3bcd557144acca3d414164f7d6d882022-12-22T03:02:33ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061999-09-0112979280210.1094/MPMI.1999.12.9.792Genetic and Physical Mapping of the RPP13 Locus, in Arabidopsis, Responsible for Specific Recognition of Several Peronospora parasitica (Downy Mildew) IsolatesPeter Bittner-EddyCanan CanNick GunnMatthieu PinelMahmut TörIan CruteEric B. HolubJim BeynonFifteen isolates of the biotrophic oomycete Peronospora parasitica (downy mildew) were obtained from a population of Arabidopsis thaliana plants that established naturally in a garden the previous year. They exhibited phenotypic variation in a set of 12 Arabidopsis accessions that suggested that the parasite population consisted of at least six pathotypes. One isolate, Maks9, elicited an interaction phenotype of flecking necrosis and no sporulation (FN) in the Arabidopsis accession Nd-1, and more extensive pitting necrosis with no sporulation (PN) in the accession Ws-2. RPP13 was designated as the locus for a single dominant resistance gene associated with the resistance in Nd-1 and mapped to an interval of approximately 60 kb on a bacterial artificial chromosome (BAC) contig on the lower arm of chromosome 3. This locus is approximately 6 cM telomeric to RPP1, which was previously described as the locus for the PN interaction with five Peronospora isolates, including resistance to Maks9 in Ws-2. New Peronospora isolates were obtained from four other geographically distinct populations of P. parasitica. Four isolates were characterized that elicited an FN phenotype in Nd-1 and mapped resistance to the RPP13 locus. This suggests that the RPP13 locus contains either a single gene capable of multiple isolate recognition or a group of tightly linked genes. Further analysis suggests that the RPP11 gene in the accession Rld-0 may be allelic to RPP13 but results in a different recognition capability.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.9.792 |
| spellingShingle | Peter Bittner-Eddy Canan Can Nick Gunn Matthieu Pinel Mahmut Tör Ian Crute Eric B. Holub Jim Beynon Genetic and Physical Mapping of the RPP13 Locus, in Arabidopsis, Responsible for Specific Recognition of Several Peronospora parasitica (Downy Mildew) Isolates Molecular Plant-Microbe Interactions |
| title | Genetic and Physical Mapping of the RPP13 Locus, in Arabidopsis, Responsible for Specific Recognition of Several Peronospora parasitica (Downy Mildew) Isolates |
| title_full | Genetic and Physical Mapping of the RPP13 Locus, in Arabidopsis, Responsible for Specific Recognition of Several Peronospora parasitica (Downy Mildew) Isolates |
| title_fullStr | Genetic and Physical Mapping of the RPP13 Locus, in Arabidopsis, Responsible for Specific Recognition of Several Peronospora parasitica (Downy Mildew) Isolates |
| title_full_unstemmed | Genetic and Physical Mapping of the RPP13 Locus, in Arabidopsis, Responsible for Specific Recognition of Several Peronospora parasitica (Downy Mildew) Isolates |
| title_short | Genetic and Physical Mapping of the RPP13 Locus, in Arabidopsis, Responsible for Specific Recognition of Several Peronospora parasitica (Downy Mildew) Isolates |
| title_sort | genetic and physical mapping of the rpp13 locus in arabidopsis responsible for specific recognition of several peronospora parasitica downy mildew isolates |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.9.792 |
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