DPP in the matrix activates AKT and mTOR signaling pathway to promote preodontoblast survival and differentiation
Dentin phosphophoryn (DPP) is an extracellular matrix protein synthesized by odontoblasts. It is highly acidic and the phosphorylated protein possesses a strong affinity for calcium ions. Therefore, DPP in the extracellular matrix can promote hydroxyapatite nucleation and can regulate the size of th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-08-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00221/full |
| _version_ | 1818271525627756544 |
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| author | ANNE eGEORGE Asha eEapen Asha eEapen |
| author_facet | ANNE eGEORGE Asha eEapen Asha eEapen |
| author_sort | ANNE eGEORGE |
| collection | DOAJ |
| description | Dentin phosphophoryn (DPP) is an extracellular matrix protein synthesized by odontoblasts. It is highly acidic and the phosphorylated protein possesses a strong affinity for calcium ions. Therefore, DPP in the extracellular matrix can promote hydroxyapatite nucleation and can regulate the size of the growing crystal. Besides its calcium binding property, DPP can initiate signaling functions from the ECM (Extracellular matrix). The signals that promote the cytodifferentiation of preodontoblasts to fully functional odontoblasts are not known. In this study, we demonstrate that preodontoblasts on a DPP matrix, generates mechanical and biochemical signals. This is initiated by the ligation of the integrins with the RGD containing DPP. The downstream biochemical response observed is the activation of the AKT( protein kinase B) and mTOR (mammalian target of rapamycin) signaling pathways leading to the activation of the transcription factor NF- κB (Nuclear factor κB) . Terminal differentiation of the preodontoblasts was assessed by identifying phosphate and calcium deposits in the matrix using von Kossa and Alizarin red staining respectively. Identifying the signaling pathways initiated by DPP in the dentin matrix would help in devising strategies for dentin tissue engineering. |
| first_indexed | 2024-12-12T21:27:34Z |
| format | Article |
| id | doaj.art-79d8d09841894e188b9e440c2d440bcb |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-12T21:27:34Z |
| publishDate | 2015-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-79d8d09841894e188b9e440c2d440bcb2022-12-22T00:11:25ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2015-08-01610.3389/fphys.2015.00221156497DPP in the matrix activates AKT and mTOR signaling pathway to promote preodontoblast survival and differentiationANNE eGEORGE0Asha eEapen1Asha eEapen2University of Illinois at ChicagoUniversity of Illinois at ChicagoSouthern Illinois University-EdwardsvilleDentin phosphophoryn (DPP) is an extracellular matrix protein synthesized by odontoblasts. It is highly acidic and the phosphorylated protein possesses a strong affinity for calcium ions. Therefore, DPP in the extracellular matrix can promote hydroxyapatite nucleation and can regulate the size of the growing crystal. Besides its calcium binding property, DPP can initiate signaling functions from the ECM (Extracellular matrix). The signals that promote the cytodifferentiation of preodontoblasts to fully functional odontoblasts are not known. In this study, we demonstrate that preodontoblasts on a DPP matrix, generates mechanical and biochemical signals. This is initiated by the ligation of the integrins with the RGD containing DPP. The downstream biochemical response observed is the activation of the AKT( protein kinase B) and mTOR (mammalian target of rapamycin) signaling pathways leading to the activation of the transcription factor NF- κB (Nuclear factor κB) . Terminal differentiation of the preodontoblasts was assessed by identifying phosphate and calcium deposits in the matrix using von Kossa and Alizarin red staining respectively. Identifying the signaling pathways initiated by DPP in the dentin matrix would help in devising strategies for dentin tissue engineering.http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00221/fullCell ProliferationOdontoblastsdifferentiationsignalingDentin phosphophorynpereodontoblasts |
| spellingShingle | ANNE eGEORGE Asha eEapen Asha eEapen DPP in the matrix activates AKT and mTOR signaling pathway to promote preodontoblast survival and differentiation Frontiers in Physiology Cell Proliferation Odontoblasts differentiation signaling Dentin phosphophoryn pereodontoblasts |
| title | DPP in the matrix activates AKT and mTOR signaling pathway to promote preodontoblast survival and differentiation |
| title_full | DPP in the matrix activates AKT and mTOR signaling pathway to promote preodontoblast survival and differentiation |
| title_fullStr | DPP in the matrix activates AKT and mTOR signaling pathway to promote preodontoblast survival and differentiation |
| title_full_unstemmed | DPP in the matrix activates AKT and mTOR signaling pathway to promote preodontoblast survival and differentiation |
| title_short | DPP in the matrix activates AKT and mTOR signaling pathway to promote preodontoblast survival and differentiation |
| title_sort | dpp in the matrix activates akt and mtor signaling pathway to promote preodontoblast survival and differentiation |
| topic | Cell Proliferation Odontoblasts differentiation signaling Dentin phosphophoryn pereodontoblasts |
| url | http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00221/full |
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