Micropropagation of <i>Vaccinium corymbosum</i> L.: An Alternative Procedure for the Production of Secondary Metabolites
In vitro culture has become a dependable approach for the mass production of plant material as the market for innovative plant-derived medicinal approaches has grown significantly. Furthermore, because it permits manipulation of biosynthetic routes to boost the production and accumulation of certain...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/6/480 |
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| author | Doina Clapa Silvia-Amalia Nemeș Floricuța Ranga Monica Hârța Dan-Cristian Vodnar Lavinia-Florina Călinoiu |
| author_facet | Doina Clapa Silvia-Amalia Nemeș Floricuța Ranga Monica Hârța Dan-Cristian Vodnar Lavinia-Florina Călinoiu |
| author_sort | Doina Clapa |
| collection | DOAJ |
| description | In vitro culture has become a dependable approach for the mass production of plant material as the market for innovative plant-derived medicinal approaches has grown significantly. Furthermore, because it permits manipulation of biosynthetic routes to boost the production and accumulation of certain compounds, this technology has enormous potential for the manufacture of natural bioactive chemicals. As a result, the goal of this study was to develop an efficient micropropagation system for biomass production and to investigate the accumulation of bioactive compounds from <i>Vaccinium corymbosum</i> L., Duke and Hortblue Petite cultivars. Two in vitro plant tissue culture systems were used for shoots production: a solid medium (5 g/L Plant agar) and liquid medium (Plantform bioreactor). The culture medium used was Woddy Plant Medium (WPM) supplemented with two growth regulators: 0.5 mg/L and 1 mg/L zeatina (Z) and 5 mg/L N6-(2-Isopentenyl) adenine (2iP). The content of phenolic compounds, carotenoids, and chlorophylls of the in vitro shoot extracts were examined via the HPLC-DAD-MS/MS technique. The results showed that cv. Hortblue Petite produced a higher amount of biomass compared with cv. Duke, on all variants of culture media in both systems (solid and liquid), while the shoots extract of the Duke variety in the liquid culture system (under all concentrations of growth regulators) had the highest content of total phenolic compounds (16,665.61 ± 424.93 μg/g). In the case of the lipophilic compounds analysed (chlorophylls and carotenoids), the solid medium reported the highest values, whereas media supplemented with 0.5 mg/L Z was proved to have the richest total content for both cultivars. |
| first_indexed | 2024-03-09T23:39:01Z |
| format | Article |
| id | doaj.art-79d8e53c2a6a4cc4bb1aa6b96e3f3128 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T23:39:01Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-79d8e53c2a6a4cc4bb1aa6b96e3f31282023-11-23T16:54:55ZengMDPI AGHorticulturae2311-75242022-05-018648010.3390/horticulturae8060480Micropropagation of <i>Vaccinium corymbosum</i> L.: An Alternative Procedure for the Production of Secondary MetabolitesDoina Clapa0Silvia-Amalia Nemeș1Floricuța Ranga2Monica Hârța3Dan-Cristian Vodnar4Lavinia-Florina Călinoiu5Institute of Advanced Horticulture Research of Transylvania, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400372 Cluj-Napoca, RomaniaInstitute of Life Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400372 Cluj-Napoca, RomaniaInstitute of Life Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400372 Cluj-Napoca, RomaniaInstitute of Life Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400372 Cluj-Napoca, RomaniaInstitute of Life Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400372 Cluj-Napoca, RomaniaFaculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400372 Cluj-Napoca, RomaniaIn vitro culture has become a dependable approach for the mass production of plant material as the market for innovative plant-derived medicinal approaches has grown significantly. Furthermore, because it permits manipulation of biosynthetic routes to boost the production and accumulation of certain compounds, this technology has enormous potential for the manufacture of natural bioactive chemicals. As a result, the goal of this study was to develop an efficient micropropagation system for biomass production and to investigate the accumulation of bioactive compounds from <i>Vaccinium corymbosum</i> L., Duke and Hortblue Petite cultivars. Two in vitro plant tissue culture systems were used for shoots production: a solid medium (5 g/L Plant agar) and liquid medium (Plantform bioreactor). The culture medium used was Woddy Plant Medium (WPM) supplemented with two growth regulators: 0.5 mg/L and 1 mg/L zeatina (Z) and 5 mg/L N6-(2-Isopentenyl) adenine (2iP). The content of phenolic compounds, carotenoids, and chlorophylls of the in vitro shoot extracts were examined via the HPLC-DAD-MS/MS technique. The results showed that cv. Hortblue Petite produced a higher amount of biomass compared with cv. Duke, on all variants of culture media in both systems (solid and liquid), while the shoots extract of the Duke variety in the liquid culture system (under all concentrations of growth regulators) had the highest content of total phenolic compounds (16,665.61 ± 424.93 μg/g). In the case of the lipophilic compounds analysed (chlorophylls and carotenoids), the solid medium reported the highest values, whereas media supplemented with 0.5 mg/L Z was proved to have the richest total content for both cultivars.https://www.mdpi.com/2311-7524/8/6/480bioreactorhighbush blueberryin vitrophenolic compoundsHPLC-DAD-MS/MS |
| spellingShingle | Doina Clapa Silvia-Amalia Nemeș Floricuța Ranga Monica Hârța Dan-Cristian Vodnar Lavinia-Florina Călinoiu Micropropagation of <i>Vaccinium corymbosum</i> L.: An Alternative Procedure for the Production of Secondary Metabolites Horticulturae bioreactor highbush blueberry in vitro phenolic compounds HPLC-DAD-MS/MS |
| title | Micropropagation of <i>Vaccinium corymbosum</i> L.: An Alternative Procedure for the Production of Secondary Metabolites |
| title_full | Micropropagation of <i>Vaccinium corymbosum</i> L.: An Alternative Procedure for the Production of Secondary Metabolites |
| title_fullStr | Micropropagation of <i>Vaccinium corymbosum</i> L.: An Alternative Procedure for the Production of Secondary Metabolites |
| title_full_unstemmed | Micropropagation of <i>Vaccinium corymbosum</i> L.: An Alternative Procedure for the Production of Secondary Metabolites |
| title_short | Micropropagation of <i>Vaccinium corymbosum</i> L.: An Alternative Procedure for the Production of Secondary Metabolites |
| title_sort | micropropagation of i vaccinium corymbosum i l an alternative procedure for the production of secondary metabolites |
| topic | bioreactor highbush blueberry in vitro phenolic compounds HPLC-DAD-MS/MS |
| url | https://www.mdpi.com/2311-7524/8/6/480 |
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