The Rice Qa-SNAREs in SYP13 Subfamily Are Involved in Regulating Arbuscular Mycorrhizal Symbiosis and Seed Fertility
Qa-SNARE gene SYP132 (isoform α) was previously reported to affect arbuscular mycorrhizal (AM) symbiosis in the legume species Medicago truncatula. In non-legumes especially monocots, it remains unknown whether certain SNARE genes are also involved in AM symbiosis. In this work, we studied a rice or...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.898286/full |
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| author | Ying-Na Liu Cheng-Chen Liu Rui Guo Li Tian Jian-Fei Cheng Ya-Nan Wu Dong Wang Bin Wang |
| author_facet | Ying-Na Liu Cheng-Chen Liu Rui Guo Li Tian Jian-Fei Cheng Ya-Nan Wu Dong Wang Bin Wang |
| author_sort | Ying-Na Liu |
| collection | DOAJ |
| description | Qa-SNARE gene SYP132 (isoform α) was previously reported to affect arbuscular mycorrhizal (AM) symbiosis in the legume species Medicago truncatula. In non-legumes especially monocots, it remains unknown whether certain SNARE genes are also involved in AM symbiosis. In this work, we studied a rice orthologous gene OsSYP132, which showed induced expression in mycorrhizal roots and two paralogous genes OsSYP131a and OsSYP131b, which were not induced by the AM fungus Rhizophagus irregularis. After employing CRISPR/Cas9 technique to generate their mutants, the Ossyp131a homozygous mutant T0 plants exhibited a dwarf phenotype and produced no fertile seeds, indicating a required role of this gene in seed fertility. Unlike the case in legume, the Ossyp132 mutants exhibited normal mycorrhizal phenotype, so did the Ossyp131b mutants. In the Ossyp131b Ossyp132 double mutants, however, the colonization rate and arbuscule abundance level decreased markedly, indicating an impaired fungal proliferation ability in rice roots. Such a defect was further confirmed by the reduced expression levels of AM marker genes. Our results in rice therefore demonstrated that while SYP13II members showed evolutionary and induction patterns specific to symbiosis, AM symbiosis is in fact controlled by the combined action of both SYP13I and SYP13II clades, revealing a functional redundancy among SYNTAXIN genes in mutualism. |
| first_indexed | 2024-12-12T10:51:50Z |
| format | Article |
| id | doaj.art-79da29531c3d43a79c359f4988449b76 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T10:51:50Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-79da29531c3d43a79c359f4988449b762022-12-22T00:26:45ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.898286898286The Rice Qa-SNAREs in SYP13 Subfamily Are Involved in Regulating Arbuscular Mycorrhizal Symbiosis and Seed FertilityYing-Na Liu0Cheng-Chen Liu1Rui Guo2Li Tian3Jian-Fei Cheng4Ya-Nan Wu5Dong Wang6Bin Wang7Department of Biology, School of Life Sciences, Nanjing University, Nanjing, ChinaDepartment of Biology, School of Life Sciences, Nanjing University, Nanjing, ChinaDepartment of Biology, School of Life Sciences, Nanjing University, Nanjing, ChinaDepartment of Biology, School of Life Sciences, Nanjing University, Nanjing, ChinaDepartment of Biology, School of Life Sciences, Nanjing University, Nanjing, ChinaDepartment of Biology, School of Life Sciences, Nanjing University, Nanjing, ChinaDepartment of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, United StatesDepartment of Biology, School of Life Sciences, Nanjing University, Nanjing, ChinaQa-SNARE gene SYP132 (isoform α) was previously reported to affect arbuscular mycorrhizal (AM) symbiosis in the legume species Medicago truncatula. In non-legumes especially monocots, it remains unknown whether certain SNARE genes are also involved in AM symbiosis. In this work, we studied a rice orthologous gene OsSYP132, which showed induced expression in mycorrhizal roots and two paralogous genes OsSYP131a and OsSYP131b, which were not induced by the AM fungus Rhizophagus irregularis. After employing CRISPR/Cas9 technique to generate their mutants, the Ossyp131a homozygous mutant T0 plants exhibited a dwarf phenotype and produced no fertile seeds, indicating a required role of this gene in seed fertility. Unlike the case in legume, the Ossyp132 mutants exhibited normal mycorrhizal phenotype, so did the Ossyp131b mutants. In the Ossyp131b Ossyp132 double mutants, however, the colonization rate and arbuscule abundance level decreased markedly, indicating an impaired fungal proliferation ability in rice roots. Such a defect was further confirmed by the reduced expression levels of AM marker genes. Our results in rice therefore demonstrated that while SYP13II members showed evolutionary and induction patterns specific to symbiosis, AM symbiosis is in fact controlled by the combined action of both SYP13I and SYP13II clades, revealing a functional redundancy among SYNTAXIN genes in mutualism.https://www.frontiersin.org/articles/10.3389/fpls.2022.898286/fullrice (Oryza sativa)arbuscular mycorrhiza (AM)SNAREOsSYP13functional redundancy |
| spellingShingle | Ying-Na Liu Cheng-Chen Liu Rui Guo Li Tian Jian-Fei Cheng Ya-Nan Wu Dong Wang Bin Wang The Rice Qa-SNAREs in SYP13 Subfamily Are Involved in Regulating Arbuscular Mycorrhizal Symbiosis and Seed Fertility Frontiers in Plant Science rice (Oryza sativa) arbuscular mycorrhiza (AM) SNARE OsSYP13 functional redundancy |
| title | The Rice Qa-SNAREs in SYP13 Subfamily Are Involved in Regulating Arbuscular Mycorrhizal Symbiosis and Seed Fertility |
| title_full | The Rice Qa-SNAREs in SYP13 Subfamily Are Involved in Regulating Arbuscular Mycorrhizal Symbiosis and Seed Fertility |
| title_fullStr | The Rice Qa-SNAREs in SYP13 Subfamily Are Involved in Regulating Arbuscular Mycorrhizal Symbiosis and Seed Fertility |
| title_full_unstemmed | The Rice Qa-SNAREs in SYP13 Subfamily Are Involved in Regulating Arbuscular Mycorrhizal Symbiosis and Seed Fertility |
| title_short | The Rice Qa-SNAREs in SYP13 Subfamily Are Involved in Regulating Arbuscular Mycorrhizal Symbiosis and Seed Fertility |
| title_sort | rice qa snares in syp13 subfamily are involved in regulating arbuscular mycorrhizal symbiosis and seed fertility |
| topic | rice (Oryza sativa) arbuscular mycorrhiza (AM) SNARE OsSYP13 functional redundancy |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.898286/full |
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