Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources
Eight isolates of Lactococcus lactis subsp. lactis were isolated and identified by phenotypic and molecular characterisation out of 23 isolates of lactic acid bacteria (LAB) from different dairy and non-dairy sources. Out of eight strains, four were obtained from dairy and four from non-dairy source...
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Czech Academy of Agricultural Sciences
2013-08-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201304-0004_isolation-identification-and-antibiotic-susceptibility-of-nis-lactococcus-lactis-from-dairy-and-non-dairy-sou.php |
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| author | Priti KHEMARIYA Sudhir SINGH Gopal NATH Anil K. GULATI |
| author_facet | Priti KHEMARIYA Sudhir SINGH Gopal NATH Anil K. GULATI |
| author_sort | Priti KHEMARIYA |
| collection | DOAJ |
| description | Eight isolates of Lactococcus lactis subsp. lactis were isolated and identified by phenotypic and molecular characterisation out of 23 isolates of lactic acid bacteria (LAB) from different dairy and non-dairy sources. Out of eight strains, four were obtained from dairy and four from non-dairy sources. All eight strains of L. lactis subsp. lactis were able to produce zones of inhibition against the Lactobacillus acidophilus NCDC 015. The antimicrobial agent produced by the isolates inhibited the growth of a range of related lactic acid bacteria and certain Gram positive food-borne microorganisms. The antimicrobial agent, i.e. nisin, produced by the strains was confirmed by PCR amplification of nisin gene sequences of 174 bp size. Antibiotic susceptibility test to 21 different types of antibiotics was evaluated. All the isolates were resistant to fosfomycin, cefepime, amikacin, kanamycin, neomycin, nalidixic acid, pipemidic acid, norfloxacin, sulphadiazine, colistin, polymixin, teicoplanin, nystatin, and amphotericin B but susceptible to ampicillin, erythromycin, spiramycin, spectinomycin, ciprofloxacin, rifampicin, and trimethoprim. |
| first_indexed | 2024-04-10T08:33:47Z |
| format | Article |
| id | doaj.art-79ddc3ca00864c30832f6e412c654a5f |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:33:47Z |
| publishDate | 2013-08-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-79ddc3ca00864c30832f6e412c654a5f2023-02-23T03:27:59ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172013-08-0131432333110.17221/316/2012-CJFScjf-201304-0004Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sourcesPriti KHEMARIYA0Sudhir SINGH1Gopal NATH2Anil K. GULATI3Post Harvest Technology Lab, Indian Institute of Vegetable Research, Jakkhini, Shahanshahpur, Varanasi (UP), IndiaPost Harvest Technology Lab, Indian Institute of Vegetable Research, Jakkhini, Shahanshahpur, Varanasi (UP), IndiaDepartment of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi (UP), IndiaDepartment of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi (UP), IndiaEight isolates of Lactococcus lactis subsp. lactis were isolated and identified by phenotypic and molecular characterisation out of 23 isolates of lactic acid bacteria (LAB) from different dairy and non-dairy sources. Out of eight strains, four were obtained from dairy and four from non-dairy sources. All eight strains of L. lactis subsp. lactis were able to produce zones of inhibition against the Lactobacillus acidophilus NCDC 015. The antimicrobial agent produced by the isolates inhibited the growth of a range of related lactic acid bacteria and certain Gram positive food-borne microorganisms. The antimicrobial agent, i.e. nisin, produced by the strains was confirmed by PCR amplification of nisin gene sequences of 174 bp size. Antibiotic susceptibility test to 21 different types of antibiotics was evaluated. All the isolates were resistant to fosfomycin, cefepime, amikacin, kanamycin, neomycin, nalidixic acid, pipemidic acid, norfloxacin, sulphadiazine, colistin, polymixin, teicoplanin, nystatin, and amphotericin B but susceptible to ampicillin, erythromycin, spiramycin, spectinomycin, ciprofloxacin, rifampicin, and trimethoprim.https://cjfs.agriculturejournals.cz/artkey/cjf-201304-0004_isolation-identification-and-antibiotic-susceptibility-of-nis-lactococcus-lactis-from-dairy-and-non-dairy-sou.phplactococcus lactis subsp. lactisantimicrobial agentnisin |
| spellingShingle | Priti KHEMARIYA Sudhir SINGH Gopal NATH Anil K. GULATI Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources Czech Journal of Food Sciences lactococcus lactis subsp. lactis antimicrobial agent nisin |
| title | Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources |
| title_full | Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources |
| title_fullStr | Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources |
| title_full_unstemmed | Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources |
| title_short | Isolation, identification and antibiotic susceptibility of nis+ Lactococcus lactis from dairy and non-dairy sources |
| title_sort | isolation identification and antibiotic susceptibility of nis lactococcus lactis from dairy and non dairy sources |
| topic | lactococcus lactis subsp. lactis antimicrobial agent nisin |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201304-0004_isolation-identification-and-antibiotic-susceptibility-of-nis-lactococcus-lactis-from-dairy-and-non-dairy-sou.php |
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