Comparative toxicity evaluation of graphene oxide (GO) and zinc oxide (ZnO) nanoparticles on Drosophila melanogaster
Engineered nanomaterials consisting of multiple nanoparticles (NPs) are finding their use in fields as wide and diverse as medicine, environment, cosmetics, energy and electronics. However, health and environmental impacts of these NPs need to be discerned individually to understand their true toxic...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Toxicology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750019302185 |
| _version_ | 1818476309365391360 |
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| author | Kritika Sood Jasreen Kaur Harpreet Singh Shailendra Kumar Arya Madhu Khatri |
| author_facet | Kritika Sood Jasreen Kaur Harpreet Singh Shailendra Kumar Arya Madhu Khatri |
| author_sort | Kritika Sood |
| collection | DOAJ |
| description | Engineered nanomaterials consisting of multiple nanoparticles (NPs) are finding their use in fields as wide and diverse as medicine, environment, cosmetics, energy and electronics. However, health and environmental impacts of these NPs need to be discerned individually to understand their true toxicity. Due to the promising application of upcoming material like GO-ZnO nanocomposite, the toxicity of ZnO and GO NPs was evaluated and compared individually in our study. This study compares the toxicity of Graphene Oxide (GO) NPs and Zinc Oxide (ZnO) NPs synthesized by Green method and Chemical method on Drosophila melanogaster. The GO, Chemical ZnO and Green ZnO NPs were synthesized and characterized using SEM, HR-TEM, FT-IR, UV–vis, EDX, XRD and DLS studies. NPs were comparatively analyzed for their cytotoxic and neurotoxic behaviors using different assays like MTT assay, mortality rate, larval crawling and climbing assay, total protein content analysis for evaluating the toxic potential of each of these NPs at different concentrations of use. Green ZnO were found to be least cytotoxic while Chemical ZnO caused the most cell damage. GO were found to have intermediary cytotoxicity. However, a different trend was observed with neurotoxicity wherein Green ZnO reportedly affected the neuromuscular coordination the most, while GO was found to have the least affect. This study provided insights into the different toxic effects caused by GO and ZnO NPs on Drosophila as well as comparative toxic effects of Chemical vs Green ZnO NPs. Keywords: Drosophila, Graphene oxide, Zinc oxide, Nanotoxicity, Nanoparticles |
| first_indexed | 2024-12-10T09:23:52Z |
| format | Article |
| id | doaj.art-79e0a30ae39f47afab7b59a9a6618eff |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-10T09:23:52Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-79e0a30ae39f47afab7b59a9a6618eff2022-12-22T01:54:35ZengElsevierToxicology Reports2214-75002019-01-016768781Comparative toxicity evaluation of graphene oxide (GO) and zinc oxide (ZnO) nanoparticles on Drosophila melanogasterKritika Sood0Jasreen Kaur1Harpreet Singh2Shailendra Kumar Arya3Madhu Khatri4Department of Biotechnology Engineering, University Institute of Engineering and Technology, Panjab University, Chandigarh, 160014, IndiaCenter for Nanoscience and Nanotechnology, Panjab University, Chandigarh, 160014, IndiaDepartment of Biotechnology Engineering, University Institute of Engineering and Technology, Panjab University, Chandigarh, 160014, IndiaDepartment of Biotechnology Engineering, University Institute of Engineering and Technology, Panjab University, Chandigarh, 160014, IndiaDepartment of Biotechnology Engineering, University Institute of Engineering and Technology, Panjab University, Chandigarh, 160014, India; Wellcome trust/DBT India Alliance Early Career Fellow, India; Corresponding author at: Department of Biotechnology Engineering, University Institute of Engineering and Technology, Panjab University, Chandigarh, 160014, India.Engineered nanomaterials consisting of multiple nanoparticles (NPs) are finding their use in fields as wide and diverse as medicine, environment, cosmetics, energy and electronics. However, health and environmental impacts of these NPs need to be discerned individually to understand their true toxicity. Due to the promising application of upcoming material like GO-ZnO nanocomposite, the toxicity of ZnO and GO NPs was evaluated and compared individually in our study. This study compares the toxicity of Graphene Oxide (GO) NPs and Zinc Oxide (ZnO) NPs synthesized by Green method and Chemical method on Drosophila melanogaster. The GO, Chemical ZnO and Green ZnO NPs were synthesized and characterized using SEM, HR-TEM, FT-IR, UV–vis, EDX, XRD and DLS studies. NPs were comparatively analyzed for their cytotoxic and neurotoxic behaviors using different assays like MTT assay, mortality rate, larval crawling and climbing assay, total protein content analysis for evaluating the toxic potential of each of these NPs at different concentrations of use. Green ZnO were found to be least cytotoxic while Chemical ZnO caused the most cell damage. GO were found to have intermediary cytotoxicity. However, a different trend was observed with neurotoxicity wherein Green ZnO reportedly affected the neuromuscular coordination the most, while GO was found to have the least affect. This study provided insights into the different toxic effects caused by GO and ZnO NPs on Drosophila as well as comparative toxic effects of Chemical vs Green ZnO NPs. Keywords: Drosophila, Graphene oxide, Zinc oxide, Nanotoxicity, Nanoparticleshttp://www.sciencedirect.com/science/article/pii/S2214750019302185 |
| spellingShingle | Kritika Sood Jasreen Kaur Harpreet Singh Shailendra Kumar Arya Madhu Khatri Comparative toxicity evaluation of graphene oxide (GO) and zinc oxide (ZnO) nanoparticles on Drosophila melanogaster Toxicology Reports |
| title | Comparative toxicity evaluation of graphene oxide (GO) and zinc oxide (ZnO) nanoparticles on Drosophila melanogaster |
| title_full | Comparative toxicity evaluation of graphene oxide (GO) and zinc oxide (ZnO) nanoparticles on Drosophila melanogaster |
| title_fullStr | Comparative toxicity evaluation of graphene oxide (GO) and zinc oxide (ZnO) nanoparticles on Drosophila melanogaster |
| title_full_unstemmed | Comparative toxicity evaluation of graphene oxide (GO) and zinc oxide (ZnO) nanoparticles on Drosophila melanogaster |
| title_short | Comparative toxicity evaluation of graphene oxide (GO) and zinc oxide (ZnO) nanoparticles on Drosophila melanogaster |
| title_sort | comparative toxicity evaluation of graphene oxide go and zinc oxide zno nanoparticles on drosophila melanogaster |
| url | http://www.sciencedirect.com/science/article/pii/S2214750019302185 |
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