Regional Differences in Heat Shock Protein 25 Expression in Brain and Spinal Cord Astrocytes of Wild-Type and SOD1 <sup>G93A</sup> Mice
Heterogeneity of glia in different CNS regions may contribute to the selective vulnerability of neuronal populations in neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS). Here, we explored regional variations in the expression of heat shock protein 25 in glia under conditions...
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MDPI AG
2021-05-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/5/1257 |
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| author | Rebecca San Gil Benjamin E. Clarke Heath Ecroyd Bernadett Kalmar Linda Greensmith |
| author_facet | Rebecca San Gil Benjamin E. Clarke Heath Ecroyd Bernadett Kalmar Linda Greensmith |
| author_sort | Rebecca San Gil |
| collection | DOAJ |
| description | Heterogeneity of glia in different CNS regions may contribute to the selective vulnerability of neuronal populations in neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS). Here, we explored regional variations in the expression of heat shock protein 25 in glia under conditions of acute and chronic stress. Hsp27 (Hsp27; murine orthologue: Hsp25) fulfils a number of cytoprotective functions and may therefore be a possible therapeutic target in ALS. We identified a subpopulation of astrocytes in primary murine mixed glial cultures that expressed Hsp25. Under basal conditions, the proportion of Hsp25-positive astrocytes was twice as high in spinal cord cultures than in cortical cultures. To explore the physiological role of the elevated Hsp25 expression in spinal cord astrocytes, we exposed cortical and spinal cord glia to acute stress, using heat stress and pro-inflammatory stimuli. Surprisingly, we observed no stress-induced increase in Hsp25 expression in either cortical or spinal cord astrocytes. Similarly, exposure to endogenous stress, as modelled in glial cultures from SOD1 <sup>G93A</sup>-ALS mice, did not increase Hsp25 expression above that observed in astrocytes from wild-type mice. In vivo, Hsp25 expression was greater under conditions of chronic stress present in the spinal cord of SOD1 <sup>G93A</sup> mice than in wild-type mice, although this increase in expression is likely to be due to the extensive gliosis that occurs in this model. Together, these results show that there are differences in the expression of Hsp25 in astrocytes in different regions of the central nervous system, but Hsp25 expression is not upregulated under acute or chronic stress conditions. |
| first_indexed | 2024-03-10T11:15:15Z |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T11:15:15Z |
| publishDate | 2021-05-01 |
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| series | Cells |
| spelling | doaj.art-79e517f5cee24ee49b3852bffbf260072023-11-21T20:28:42ZengMDPI AGCells2073-44092021-05-01105125710.3390/cells10051257Regional Differences in Heat Shock Protein 25 Expression in Brain and Spinal Cord Astrocytes of Wild-Type and SOD1 <sup>G93A</sup> MiceRebecca San Gil0Benjamin E. Clarke1Heath Ecroyd2Bernadett Kalmar3Linda Greensmith4Molecular Horizons and School of Chemistry and Molecular Bioscience, Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2519, AustraliaDepartment of Neuromuscular Disease, UCL Queen Square Institute of Neurology, London WC1N 3BG, UKMolecular Horizons and School of Chemistry and Molecular Bioscience, Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW 2519, AustraliaDepartment of Neuromuscular Disease, UCL Queen Square Institute of Neurology, London WC1N 3BG, UKDepartment of Neuromuscular Disease, UCL Queen Square Institute of Neurology, London WC1N 3BG, UKHeterogeneity of glia in different CNS regions may contribute to the selective vulnerability of neuronal populations in neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS). Here, we explored regional variations in the expression of heat shock protein 25 in glia under conditions of acute and chronic stress. Hsp27 (Hsp27; murine orthologue: Hsp25) fulfils a number of cytoprotective functions and may therefore be a possible therapeutic target in ALS. We identified a subpopulation of astrocytes in primary murine mixed glial cultures that expressed Hsp25. Under basal conditions, the proportion of Hsp25-positive astrocytes was twice as high in spinal cord cultures than in cortical cultures. To explore the physiological role of the elevated Hsp25 expression in spinal cord astrocytes, we exposed cortical and spinal cord glia to acute stress, using heat stress and pro-inflammatory stimuli. Surprisingly, we observed no stress-induced increase in Hsp25 expression in either cortical or spinal cord astrocytes. Similarly, exposure to endogenous stress, as modelled in glial cultures from SOD1 <sup>G93A</sup>-ALS mice, did not increase Hsp25 expression above that observed in astrocytes from wild-type mice. In vivo, Hsp25 expression was greater under conditions of chronic stress present in the spinal cord of SOD1 <sup>G93A</sup> mice than in wild-type mice, although this increase in expression is likely to be due to the extensive gliosis that occurs in this model. Together, these results show that there are differences in the expression of Hsp25 in astrocytes in different regions of the central nervous system, but Hsp25 expression is not upregulated under acute or chronic stress conditions.https://www.mdpi.com/2073-4409/10/5/1257heat shock responseheat shock protein 27gliaastrocytesamyotrophic lateral sclerosismotor neuron disease |
| spellingShingle | Rebecca San Gil Benjamin E. Clarke Heath Ecroyd Bernadett Kalmar Linda Greensmith Regional Differences in Heat Shock Protein 25 Expression in Brain and Spinal Cord Astrocytes of Wild-Type and SOD1 <sup>G93A</sup> Mice Cells heat shock response heat shock protein 27 glia astrocytes amyotrophic lateral sclerosis motor neuron disease |
| title | Regional Differences in Heat Shock Protein 25 Expression in Brain and Spinal Cord Astrocytes of Wild-Type and SOD1 <sup>G93A</sup> Mice |
| title_full | Regional Differences in Heat Shock Protein 25 Expression in Brain and Spinal Cord Astrocytes of Wild-Type and SOD1 <sup>G93A</sup> Mice |
| title_fullStr | Regional Differences in Heat Shock Protein 25 Expression in Brain and Spinal Cord Astrocytes of Wild-Type and SOD1 <sup>G93A</sup> Mice |
| title_full_unstemmed | Regional Differences in Heat Shock Protein 25 Expression in Brain and Spinal Cord Astrocytes of Wild-Type and SOD1 <sup>G93A</sup> Mice |
| title_short | Regional Differences in Heat Shock Protein 25 Expression in Brain and Spinal Cord Astrocytes of Wild-Type and SOD1 <sup>G93A</sup> Mice |
| title_sort | regional differences in heat shock protein 25 expression in brain and spinal cord astrocytes of wild type and sod1 sup g93a sup mice |
| topic | heat shock response heat shock protein 27 glia astrocytes amyotrophic lateral sclerosis motor neuron disease |
| url | https://www.mdpi.com/2073-4409/10/5/1257 |
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