Impact of Proton Beam Irradiation on the Growth and Biochemical Indexes of Barley (<i>Hordeum vulgare</i> L.) Seedlings Grown under Salt Stress
The present paper examines the effects of salt stress on the growth, pigments, lipid peroxidation and antioxidant ability of barley (<i>Hordeum vulgare</i> L.) seedlings raised from proton beam irradiated caryopses. In order to assess the effects of radiation on the early stages of plant...
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MDPI AG
2020-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/9/1234 |
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| author | Lacramioara Oprica Marius-Nicusor Grigore Iulia Caraciuc Daniela Gherghel Cosmin-Teodor Mihai Gabriela Vochita |
| author_facet | Lacramioara Oprica Marius-Nicusor Grigore Iulia Caraciuc Daniela Gherghel Cosmin-Teodor Mihai Gabriela Vochita |
| author_sort | Lacramioara Oprica |
| collection | DOAJ |
| description | The present paper examines the effects of salt stress on the growth, pigments, lipid peroxidation and antioxidant ability of barley (<i>Hordeum vulgare</i> L.) seedlings raised from proton beam irradiated caryopses. In order to assess the effects of radiation on the early stages of plant growth and analyze its possible influence on the alleviation of salinity, 3 and 5 Gy doses were used on dried barley seeds and germination occurred in the presence/absence of NaCl (100 mM and 200 mM). After treatment, photosynthetic pigments increased in the 5 Gy variant, which registered a higher value than the control. Among the antioxidant enzymes studied (SOD, CAT, and POD) only CAT activity increased in proton beam irradiated seeds germinated under salinity conditions, which indicates the activation of antioxidant defense. The malondialdehyde (MDA) content declined with the increase of irradiation doses on seeds germinated at 200 mM NaCl. On the other hand, the concentration of 200 mM NaCl applied alone or combined with radiation revealed an increase in soluble protein content. The growth rate suggests that 3 Gy proton beam irradiation of barley seeds can alleviate the harmful effects of 100 mM NaCl salinity, given that seedlings’ growth rate increased by 1.95% compared to the control. |
| first_indexed | 2024-03-10T16:13:37Z |
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| id | doaj.art-79e9e576566c42c2a6f2cc1e45d21d13 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T16:13:37Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-79e9e576566c42c2a6f2cc1e45d21d132023-11-20T14:14:59ZengMDPI AGPlants2223-77472020-09-0199123410.3390/plants9091234Impact of Proton Beam Irradiation on the Growth and Biochemical Indexes of Barley (<i>Hordeum vulgare</i> L.) Seedlings Grown under Salt StressLacramioara Oprica0Marius-Nicusor Grigore1Iulia Caraciuc2Daniela Gherghel3Cosmin-Teodor Mihai4Gabriela Vochita5Faculty of Biology, Alexandru Ioan Cuza University, 20A Carol I Bd., 700506 Iasi, RomaniaFaculty of Biology, Alexandru Ioan Cuza University, 20A Carol I Bd., 700506 Iasi, RomaniaNational Institute for Research and Development in Electrical Engineering ICPE-CA, 313 Splaiul Unirii, District 3, 030138 Bucharest, RomaniaInstitute of Biological Research—Branch of NIRDBS, 47 Lascar Catargi Street, 700107 Iasi, RomaniaInstitute of Biological Research—Branch of NIRDBS, 47 Lascar Catargi Street, 700107 Iasi, RomaniaInstitute of Biological Research—Branch of NIRDBS, 47 Lascar Catargi Street, 700107 Iasi, RomaniaThe present paper examines the effects of salt stress on the growth, pigments, lipid peroxidation and antioxidant ability of barley (<i>Hordeum vulgare</i> L.) seedlings raised from proton beam irradiated caryopses. In order to assess the effects of radiation on the early stages of plant growth and analyze its possible influence on the alleviation of salinity, 3 and 5 Gy doses were used on dried barley seeds and germination occurred in the presence/absence of NaCl (100 mM and 200 mM). After treatment, photosynthetic pigments increased in the 5 Gy variant, which registered a higher value than the control. Among the antioxidant enzymes studied (SOD, CAT, and POD) only CAT activity increased in proton beam irradiated seeds germinated under salinity conditions, which indicates the activation of antioxidant defense. The malondialdehyde (MDA) content declined with the increase of irradiation doses on seeds germinated at 200 mM NaCl. On the other hand, the concentration of 200 mM NaCl applied alone or combined with radiation revealed an increase in soluble protein content. The growth rate suggests that 3 Gy proton beam irradiation of barley seeds can alleviate the harmful effects of 100 mM NaCl salinity, given that seedlings’ growth rate increased by 1.95% compared to the control.https://www.mdpi.com/2223-7747/9/9/1234beam proton radiationsaline stressantioxidant protection systemsbarley |
| spellingShingle | Lacramioara Oprica Marius-Nicusor Grigore Iulia Caraciuc Daniela Gherghel Cosmin-Teodor Mihai Gabriela Vochita Impact of Proton Beam Irradiation on the Growth and Biochemical Indexes of Barley (<i>Hordeum vulgare</i> L.) Seedlings Grown under Salt Stress Plants beam proton radiation saline stress antioxidant protection systems barley |
| title | Impact of Proton Beam Irradiation on the Growth and Biochemical Indexes of Barley (<i>Hordeum vulgare</i> L.) Seedlings Grown under Salt Stress |
| title_full | Impact of Proton Beam Irradiation on the Growth and Biochemical Indexes of Barley (<i>Hordeum vulgare</i> L.) Seedlings Grown under Salt Stress |
| title_fullStr | Impact of Proton Beam Irradiation on the Growth and Biochemical Indexes of Barley (<i>Hordeum vulgare</i> L.) Seedlings Grown under Salt Stress |
| title_full_unstemmed | Impact of Proton Beam Irradiation on the Growth and Biochemical Indexes of Barley (<i>Hordeum vulgare</i> L.) Seedlings Grown under Salt Stress |
| title_short | Impact of Proton Beam Irradiation on the Growth and Biochemical Indexes of Barley (<i>Hordeum vulgare</i> L.) Seedlings Grown under Salt Stress |
| title_sort | impact of proton beam irradiation on the growth and biochemical indexes of barley i hordeum vulgare i l seedlings grown under salt stress |
| topic | beam proton radiation saline stress antioxidant protection systems barley |
| url | https://www.mdpi.com/2223-7747/9/9/1234 |
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