Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency
Summary: Here, we report the scalable fabrication of 2i-functionalized micro-pyramid-array (μPyA/+2i) inserts for use in commercial multi-well plates, as the alternative cultivation platform for maintaining long-term self-renewal and pluripotency of multiple mESCs and mouse induced pluripotent stem...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223005230 |
| _version_ | 1797851309072711680 |
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| author | Qian Ban Wenqiao Hui Quanchao Duan Wenfei Liu Meng Zhang Xiaofeng Li Hanyue Kang Zhenni Shi Yan Zhang Jinbin Hou Xiuzhen Xu Shoudong Ye Baowei Zhang Xiaobin Xu |
| author_facet | Qian Ban Wenqiao Hui Quanchao Duan Wenfei Liu Meng Zhang Xiaofeng Li Hanyue Kang Zhenni Shi Yan Zhang Jinbin Hou Xiuzhen Xu Shoudong Ye Baowei Zhang Xiaobin Xu |
| author_sort | Qian Ban |
| collection | DOAJ |
| description | Summary: Here, we report the scalable fabrication of 2i-functionalized micro-pyramid-array (μPyA/+2i) inserts for use in commercial multi-well plates, as the alternative cultivation platform for maintaining long-term self-renewal and pluripotency of multiple mESCs and mouse induced pluripotent stem cells. Relevant evidence including cell morphology characterization increased alkaline phosphatase activity, high expression of mESC self-renewal markers, decreased levels of differentiation-associated markers, and high proportion of self-renewal marker cells are provided. Further studies demonstrated that μPyA/+2i could cause a higher cell density in mESC colony, and induce gene expression changes. Subsequent studies showed that μPyA/+2i can influence the cytoskeleton and promote cell adhesion through Cldn-7 upregulation. In summary, these μPyA/+2i inserts offer flexible and gelatin-free micro-envriomnets to maintain long-term self-renewal and pluripotency of mESCs. Enabled by the microstructured inserst, the facile stem cell manipulation and transfer among culture dishes will broaden stem cells both in routine and translational applications. |
| first_indexed | 2024-04-09T19:14:44Z |
| format | Article |
| id | doaj.art-79ec28fcd0ae4272b95863f5d7c95ab7 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T19:14:44Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-79ec28fcd0ae4272b95863f5d7c95ab72023-04-06T06:12:35ZengElsevieriScience2589-00422023-04-01264106446Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotencyQian Ban0Wenqiao Hui1Quanchao Duan2Wenfei Liu3Meng Zhang4Xiaofeng Li5Hanyue Kang6Zhenni Shi7Yan Zhang8Jinbin Hou9Xiuzhen Xu10Shoudong Ye11Baowei Zhang12Xiaobin Xu13Center for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China; Key Laboratory of Advanced Civil Engineering Materials of Ministry of Education, Key Laboratory of D&A for Metal-Functional Materials, School of Materials Science & Engineering, Tongji University, Shanghai 201804 ChinaAnhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agriculture Sciences, Hefei, Anhui 230031, ChinaCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, ChinaCalifornia NanoSystems Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USACenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, ChinaCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, ChinaKey Laboratory of Advanced Civil Engineering Materials of Ministry of Education, Key Laboratory of D&A for Metal-Functional Materials, School of Materials Science & Engineering, Tongji University, Shanghai 201804 ChinaCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, ChinaCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, ChinaCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, ChinaKey Laboratory of Advanced Civil Engineering Materials of Ministry of Education, Key Laboratory of D&A for Metal-Functional Materials, School of Materials Science & Engineering, Tongji University, Shanghai 201804 ChinaCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China; Corresponding authorCenter for Stem Cell and Translational Medicine, School of Life Science, Anhui University, Hefei, Anhui 230601, China; Corresponding authorKey Laboratory of Advanced Civil Engineering Materials of Ministry of Education, Key Laboratory of D&A for Metal-Functional Materials, School of Materials Science & Engineering, Tongji University, Shanghai 201804 China; Corresponding authorSummary: Here, we report the scalable fabrication of 2i-functionalized micro-pyramid-array (μPyA/+2i) inserts for use in commercial multi-well plates, as the alternative cultivation platform for maintaining long-term self-renewal and pluripotency of multiple mESCs and mouse induced pluripotent stem cells. Relevant evidence including cell morphology characterization increased alkaline phosphatase activity, high expression of mESC self-renewal markers, decreased levels of differentiation-associated markers, and high proportion of self-renewal marker cells are provided. Further studies demonstrated that μPyA/+2i could cause a higher cell density in mESC colony, and induce gene expression changes. Subsequent studies showed that μPyA/+2i can influence the cytoskeleton and promote cell adhesion through Cldn-7 upregulation. In summary, these μPyA/+2i inserts offer flexible and gelatin-free micro-envriomnets to maintain long-term self-renewal and pluripotency of mESCs. Enabled by the microstructured inserst, the facile stem cell manipulation and transfer among culture dishes will broaden stem cells both in routine and translational applications.http://www.sciencedirect.com/science/article/pii/S2589004223005230Stem cells researchBiomaterials |
| spellingShingle | Qian Ban Wenqiao Hui Quanchao Duan Wenfei Liu Meng Zhang Xiaofeng Li Hanyue Kang Zhenni Shi Yan Zhang Jinbin Hou Xiuzhen Xu Shoudong Ye Baowei Zhang Xiaobin Xu Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency iScience Stem cells research Biomaterials |
| title | Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency |
| title_full | Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency |
| title_fullStr | Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency |
| title_full_unstemmed | Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency |
| title_short | Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency |
| title_sort | commercial dishes with gelatin free microstructured inserts for elongated stem cell self renewal and pluripotency |
| topic | Stem cells research Biomaterials |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223005230 |
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