Global gene analysis identifying genes commonly regulated by the Ras/Raf/MEK and type I IFN pathways
Oncolytic viruses exploit alterations in cancer cells to specifically infect cancer cells but not normal healthy cells. Previous work has shown that oncogenic Ras interferes with interferon (IFN) signaling to promote viral replication. Furthermore, inhibition of the Ras/Raf/MEK/ERK pathway at the le...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Genomics Data |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221359601500029X |
| _version_ | 1818034036760641536 |
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| author | Y. Komatsu K. Hirasawa S.L. Christian |
| author_facet | Y. Komatsu K. Hirasawa S.L. Christian |
| author_sort | Y. Komatsu |
| collection | DOAJ |
| description | Oncolytic viruses exploit alterations in cancer cells to specifically infect cancer cells but not normal healthy cells. Previous work has shown that oncogenic Ras interferes with interferon (IFN) signaling to promote viral replication. Furthermore, inhibition of the Ras/Raf/MEK/ERK pathway at the level of Ras, MEK, or ERK was sufficient to restore IFN signaling. In order to identify genes that were commonly regulated by the inhibition of the Ras pathway and the IFN pathway, we treated NIH/3T3 cells that overexpress oncogenic Ras with the MEK inhibitor, U0126, or IFN-α for 6 h, and performed DNA microarray analysis (Gene Expression Omnibus accession number GSE49469). Here, we also provide additional information on the experimental and functional analysis of the genes responsive to U0126 and IFN. |
| first_indexed | 2024-12-10T06:32:47Z |
| format | Article |
| id | doaj.art-79ee981ae38e49f7a0719091a4d449aa |
| institution | Directory Open Access Journal |
| issn | 2213-5960 |
| language | English |
| last_indexed | 2024-12-10T06:32:47Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Genomics Data |
| spelling | doaj.art-79ee981ae38e49f7a0719091a4d449aa2022-12-22T01:59:02ZengElsevierGenomics Data2213-59602015-06-014C848710.1016/j.gdata.2015.03.012Global gene analysis identifying genes commonly regulated by the Ras/Raf/MEK and type I IFN pathwaysY. Komatsu0K. Hirasawa1S.L. Christian2Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, CanadaDivision of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, CanadaDivision of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, CanadaOncolytic viruses exploit alterations in cancer cells to specifically infect cancer cells but not normal healthy cells. Previous work has shown that oncogenic Ras interferes with interferon (IFN) signaling to promote viral replication. Furthermore, inhibition of the Ras/Raf/MEK/ERK pathway at the level of Ras, MEK, or ERK was sufficient to restore IFN signaling. In order to identify genes that were commonly regulated by the inhibition of the Ras pathway and the IFN pathway, we treated NIH/3T3 cells that overexpress oncogenic Ras with the MEK inhibitor, U0126, or IFN-α for 6 h, and performed DNA microarray analysis (Gene Expression Omnibus accession number GSE49469). Here, we also provide additional information on the experimental and functional analysis of the genes responsive to U0126 and IFN.http://www.sciencedirect.com/science/article/pii/S221359601500029XOncogenic RasRas/Raf/MEKU0126Type I interferonNIH/3T3 |
| spellingShingle | Y. Komatsu K. Hirasawa S.L. Christian Global gene analysis identifying genes commonly regulated by the Ras/Raf/MEK and type I IFN pathways Genomics Data Oncogenic Ras Ras/Raf/MEK U0126 Type I interferon NIH/3T3 |
| title | Global gene analysis identifying genes commonly regulated by the Ras/Raf/MEK and type I IFN pathways |
| title_full | Global gene analysis identifying genes commonly regulated by the Ras/Raf/MEK and type I IFN pathways |
| title_fullStr | Global gene analysis identifying genes commonly regulated by the Ras/Raf/MEK and type I IFN pathways |
| title_full_unstemmed | Global gene analysis identifying genes commonly regulated by the Ras/Raf/MEK and type I IFN pathways |
| title_short | Global gene analysis identifying genes commonly regulated by the Ras/Raf/MEK and type I IFN pathways |
| title_sort | global gene analysis identifying genes commonly regulated by the ras raf mek and type i ifn pathways |
| topic | Oncogenic Ras Ras/Raf/MEK U0126 Type I interferon NIH/3T3 |
| url | http://www.sciencedirect.com/science/article/pii/S221359601500029X |
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