The First Investigation of West Nile Virus in Horses Using Real Time RT-PCR in Middle Black Sea Region in Turkey
Background: West Nile Virus (WNV) is a mosquito-borne disease that can cause fatal infection in mammals in-cluding humans, dogs, horses, birds and reptiles. Although West Nile Virus is an asymptomatic infection, especially it can cause neurologic disorders in humans and horses. The aim of this study...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-10-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/171 |
| _version_ | 1828428377584828416 |
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| author | Zafer Yazici Harun Albayrak Emre Ozan Semra Gumusova |
| author_facet | Zafer Yazici Harun Albayrak Emre Ozan Semra Gumusova |
| author_sort | Zafer Yazici |
| collection | DOAJ |
| description | Background: West Nile Virus (WNV) is a mosquito-borne disease that can cause fatal infection in mammals in-cluding humans, dogs, horses, birds and reptiles. Although West Nile Virus is an asymptomatic infection, especially it can cause neurologic disorders in humans and horses. The aim of this study was to the investigate virological pres- ence of WNV in horses in the Black Sea Region of Turkey using real time RT-PCR (rRT-PCR).
Methods: Totally, 120 horse sera were collected equally from 4 provinces in Middle Black Sea Region of Turkey and investigated for WNV presence by Taqman based rRT-PCR.
Results: WNV nucleic acid was not detected in any horse serum sample.
Conclusion: Although obtained result indicated no evidence of WNV–RNA in horses, Black Sea Region of Turkey is one of the suitable places for the WNV infection. For this reason, our research will continue for the determination of the viruses in vectors and susceptible animals such as horses, dogs, etc. |
| first_indexed | 2024-12-10T17:17:47Z |
| format | Article |
| id | doaj.art-79f12f047ca34099a1dcfed6897ca6eb |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-10T17:17:47Z |
| publishDate | 2015-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-79f12f047ca34099a1dcfed6897ca6eb2022-12-22T01:40:05ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712015-10-0162The First Investigation of West Nile Virus in Horses Using Real Time RT-PCR in Middle Black Sea Region in TurkeyZafer Yazici0Harun Albayrak1Emre Ozan2Semra Gumusova3Department of Virology, Faculty of Veterinary Medicine,Ondokuz Mayis University, Samsun, TurkeyDepartment of Virology, Faculty of Veterinary Medicine,Ondokuz Mayis University, Samsun, TurkeyVirology Laboratory, Regional Veterinary Control and Research Institute, Ministry of Agriculture and Rural Affairs, Samsun, TurkeyDepartment of Virology, Faculty of Veterinary Medicine,Ondokuz Mayis University, Samsun, TurkeyBackground: West Nile Virus (WNV) is a mosquito-borne disease that can cause fatal infection in mammals in-cluding humans, dogs, horses, birds and reptiles. Although West Nile Virus is an asymptomatic infection, especially it can cause neurologic disorders in humans and horses. The aim of this study was to the investigate virological pres- ence of WNV in horses in the Black Sea Region of Turkey using real time RT-PCR (rRT-PCR). Methods: Totally, 120 horse sera were collected equally from 4 provinces in Middle Black Sea Region of Turkey and investigated for WNV presence by Taqman based rRT-PCR. Results: WNV nucleic acid was not detected in any horse serum sample. Conclusion: Although obtained result indicated no evidence of WNV–RNA in horses, Black Sea Region of Turkey is one of the suitable places for the WNV infection. For this reason, our research will continue for the determination of the viruses in vectors and susceptible animals such as horses, dogs, etc.https://jad.tums.ac.ir/index.php/jad/article/view/171HorsesRT-PCRSeraWest Nile Virus |
| spellingShingle | Zafer Yazici Harun Albayrak Emre Ozan Semra Gumusova The First Investigation of West Nile Virus in Horses Using Real Time RT-PCR in Middle Black Sea Region in Turkey Journal of Arthropod-Borne Diseases Horses RT-PCR Sera West Nile Virus |
| title | The First Investigation of West Nile Virus in Horses Using Real Time RT-PCR in Middle Black Sea Region in Turkey |
| title_full | The First Investigation of West Nile Virus in Horses Using Real Time RT-PCR in Middle Black Sea Region in Turkey |
| title_fullStr | The First Investigation of West Nile Virus in Horses Using Real Time RT-PCR in Middle Black Sea Region in Turkey |
| title_full_unstemmed | The First Investigation of West Nile Virus in Horses Using Real Time RT-PCR in Middle Black Sea Region in Turkey |
| title_short | The First Investigation of West Nile Virus in Horses Using Real Time RT-PCR in Middle Black Sea Region in Turkey |
| title_sort | first investigation of west nile virus in horses using real time rt pcr in middle black sea region in turkey |
| topic | Horses RT-PCR Sera West Nile Virus |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/171 |
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