Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome
The gut microbiota is engaged in multiple interactions affecting host health, and gut dysbiosis can lead to many diseases. However, the effects of acetylcysteine (NAC) on the gut microbiome composition in pigs using metagenomic sequencing have not been reported. In this study, we used metagenome seq...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2036-7481/14/4/132 |
| _version_ | 1797380059003092992 |
|---|---|
| author | Shulin Fu Xinyue Tian Jingyang Li Yuzhen Yuan Jing He Chun Peng Ling Guo Chun Ye Yu Liu Bingbing Zong Yinsheng Qiu |
| author_facet | Shulin Fu Xinyue Tian Jingyang Li Yuzhen Yuan Jing He Chun Peng Ling Guo Chun Ye Yu Liu Bingbing Zong Yinsheng Qiu |
| author_sort | Shulin Fu |
| collection | DOAJ |
| description | The gut microbiota is engaged in multiple interactions affecting host health, and gut dysbiosis can lead to many diseases. However, the effects of acetylcysteine (NAC) on the gut microbiome composition in pigs using metagenomic sequencing have not been reported. In this study, we used metagenome sequencing to study the effects of NAC on the pig gut microbiome. Sequencing results showed that microbial diversity was changed after NAC treatment. Antibiotic Resistance Genes Database (ARDB) analysis demonstrated that the main genes modified were <i>macb</i>, <i>tsnr</i>, <i>norm</i>, <i>bl2be-per</i>, <i>vansb</i> and <i>pbp1b</i> in the NAC group. Our data showed that NAC could affect microbial distribution at the phylum, gene and species levels. At the species level, NAC significantly increased the abundances of <i>Megasphaera</i>, <i>Lactobacillus reuteri</i> and <i>Megasphaeraelsdenii</i> and reduced the abundances of <i>Phascolarctobacterium succinatutens</i>, <i>Prevotellacopri</i> and <i>Selenomonasbovis</i> compared with the control group. In addition, Gene Ontology (GO) analysis revealed that in the NAC group, cellular process, metabolic process and single-organism process were the dominant terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that RNA transport, MAPK signaling pathway, cell cycle, glycosylphosphatidylinositol (GPI)-anchor biosynthesis and VEGF signaling pathway were the dominant signaling pathways in the NAC group. In conclusion, our results suggest that NAC may modify the piglet gut microbiome composition and these findings might provide a new strategy for maintaining animal and human health in the future. |
| first_indexed | 2024-03-08T20:31:59Z |
| format | Article |
| id | doaj.art-79f2b20298914cf7aa002076b167ecd9 |
| institution | Directory Open Access Journal |
| issn | 2036-7481 |
| language | English |
| last_indexed | 2024-03-08T20:31:59Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj.art-79f2b20298914cf7aa002076b167ecd92023-12-22T14:25:07ZengMDPI AGMicrobiology Research2036-74812023-11-011441956196810.3390/microbiolres14040132Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut MicrobiomeShulin Fu0Xinyue Tian1Jingyang Li2Yuzhen Yuan3Jing He4Chun Peng5Ling Guo6Chun Ye7Yu Liu8Bingbing Zong9Yinsheng Qiu10Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, ChinaThe gut microbiota is engaged in multiple interactions affecting host health, and gut dysbiosis can lead to many diseases. However, the effects of acetylcysteine (NAC) on the gut microbiome composition in pigs using metagenomic sequencing have not been reported. In this study, we used metagenome sequencing to study the effects of NAC on the pig gut microbiome. Sequencing results showed that microbial diversity was changed after NAC treatment. Antibiotic Resistance Genes Database (ARDB) analysis demonstrated that the main genes modified were <i>macb</i>, <i>tsnr</i>, <i>norm</i>, <i>bl2be-per</i>, <i>vansb</i> and <i>pbp1b</i> in the NAC group. Our data showed that NAC could affect microbial distribution at the phylum, gene and species levels. At the species level, NAC significantly increased the abundances of <i>Megasphaera</i>, <i>Lactobacillus reuteri</i> and <i>Megasphaeraelsdenii</i> and reduced the abundances of <i>Phascolarctobacterium succinatutens</i>, <i>Prevotellacopri</i> and <i>Selenomonasbovis</i> compared with the control group. In addition, Gene Ontology (GO) analysis revealed that in the NAC group, cellular process, metabolic process and single-organism process were the dominant terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that RNA transport, MAPK signaling pathway, cell cycle, glycosylphosphatidylinositol (GPI)-anchor biosynthesis and VEGF signaling pathway were the dominant signaling pathways in the NAC group. In conclusion, our results suggest that NAC may modify the piglet gut microbiome composition and these findings might provide a new strategy for maintaining animal and human health in the future.https://www.mdpi.com/2036-7481/14/4/132acetylcysteinegut microbiomepigmetagenomic sequencingmodification |
| spellingShingle | Shulin Fu Xinyue Tian Jingyang Li Yuzhen Yuan Jing He Chun Peng Ling Guo Chun Ye Yu Liu Bingbing Zong Yinsheng Qiu Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome Microbiology Research acetylcysteine gut microbiome pig metagenomic sequencing modification |
| title | Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome |
| title_full | Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome |
| title_fullStr | Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome |
| title_full_unstemmed | Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome |
| title_short | Metagenomic Sequencing Analysis of the Effects of Acetylcysteine on the Pig Gut Microbiome |
| title_sort | metagenomic sequencing analysis of the effects of acetylcysteine on the pig gut microbiome |
| topic | acetylcysteine gut microbiome pig metagenomic sequencing modification |
| url | https://www.mdpi.com/2036-7481/14/4/132 |
| work_keys_str_mv | AT shulinfu metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome AT xinyuetian metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome AT jingyangli metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome AT yuzhenyuan metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome AT jinghe metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome AT chunpeng metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome AT lingguo metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome AT chunye metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome AT yuliu metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome AT bingbingzong metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome AT yinshengqiu metagenomicsequencinganalysisoftheeffectsofacetylcysteineonthepiggutmicrobiome |