Development of Nanomaterial-Modified Impedimetric Aptasensor—A Single-Step Strategy for 3,4-Methylenedioxymethylamphetamine Detection
Developing rapid, sensitive detection methods for 3,4-Methylenedioxymethylamphetamine (MDMA) is crucial to reduce its current misuse in the world population. With that aim, we developed an aptamer-modified tin nanoparticle (SnNP)-based nanoarchitecture as an electrochemical sensor in this study. Thi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/7/538 |
| _version_ | 1797407228733423616 |
|---|---|
| author | Shringika Soni Utkarsh Jain Donald H. Burke Nidhi Chauhan |
| author_facet | Shringika Soni Utkarsh Jain Donald H. Burke Nidhi Chauhan |
| author_sort | Shringika Soni |
| collection | DOAJ |
| description | Developing rapid, sensitive detection methods for 3,4-Methylenedioxymethylamphetamine (MDMA) is crucial to reduce its current misuse in the world population. With that aim, we developed an aptamer-modified tin nanoparticle (SnNP)-based nanoarchitecture as an electrochemical sensor in this study. This platform exhibited a high electron transfer rate with enhanced conductivity arising from its large surface area in comparison to the bare electrode. This observation was explained by the 40-fold higher electroactive surface area of SnNPs@Au, which provided a large space for 1.0 μM <i>Apt<sub>MDMA</sub></i> (0.68 ± 0.36 × 10<sup>12</sup> molecule/cm<sup>2</sup>) immobilization and yielded a significant electrochemical response in the presence of MDMA. Furthermore, the <i>Apt<sub>MDMA</sub></i>-modified SnNPs@Au sensing platform proved to be a simple yet ultrasensitive analytical device for MDMA detection in spiked biological and water samples. This novel electrochemical aptasensor showed good linearity in the range of 0.01–1.0 nM for MDMA (R<sup>2</sup> = 0.97) with a limit of detection of 0.33 nM and a sensitivity of 0.54 ohm/nM. In addition, the device showed high accuracy and stability along with signal recoveries in the range of 92–96.7% (Relative Standard Deviation, RSD, 1.1–2.18%). In conclusion, the proposed aptasensor developed here is the first to combine SnNPs and aptamers for illicit compound detection, and it offers a reliable platform for recreational drug detection. |
| first_indexed | 2024-03-09T03:38:30Z |
| format | Article |
| id | doaj.art-79f464d871a14d42b29fe7eae80a146b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T03:38:30Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-79f464d871a14d42b29fe7eae80a146b2023-12-03T14:44:28ZengMDPI AGBiosensors2079-63742022-07-0112753810.3390/bios12070538Development of Nanomaterial-Modified Impedimetric Aptasensor—A Single-Step Strategy for 3,4-Methylenedioxymethylamphetamine DetectionShringika Soni0Utkarsh Jain1Donald H. Burke2Nidhi Chauhan3Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Sector-125, Noida 201313, Uttar Pradesh, IndiaAmity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Sector-125, Noida 201313, Uttar Pradesh, IndiaDepartment of Molecular Microbiology and Immunology, Department of Biochemistry, and Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USAAmity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Sector-125, Noida 201313, Uttar Pradesh, IndiaDeveloping rapid, sensitive detection methods for 3,4-Methylenedioxymethylamphetamine (MDMA) is crucial to reduce its current misuse in the world population. With that aim, we developed an aptamer-modified tin nanoparticle (SnNP)-based nanoarchitecture as an electrochemical sensor in this study. This platform exhibited a high electron transfer rate with enhanced conductivity arising from its large surface area in comparison to the bare electrode. This observation was explained by the 40-fold higher electroactive surface area of SnNPs@Au, which provided a large space for 1.0 μM <i>Apt<sub>MDMA</sub></i> (0.68 ± 0.36 × 10<sup>12</sup> molecule/cm<sup>2</sup>) immobilization and yielded a significant electrochemical response in the presence of MDMA. Furthermore, the <i>Apt<sub>MDMA</sub></i>-modified SnNPs@Au sensing platform proved to be a simple yet ultrasensitive analytical device for MDMA detection in spiked biological and water samples. This novel electrochemical aptasensor showed good linearity in the range of 0.01–1.0 nM for MDMA (R<sup>2</sup> = 0.97) with a limit of detection of 0.33 nM and a sensitivity of 0.54 ohm/nM. In addition, the device showed high accuracy and stability along with signal recoveries in the range of 92–96.7% (Relative Standard Deviation, RSD, 1.1–2.18%). In conclusion, the proposed aptasensor developed here is the first to combine SnNPs and aptamers for illicit compound detection, and it offers a reliable platform for recreational drug detection.https://www.mdpi.com/2079-6374/12/7/5383,4-methylenedioxymethylamphetamineelectrochemical sensoraptamertin nanoparticlesrecreational drugsforensic |
| spellingShingle | Shringika Soni Utkarsh Jain Donald H. Burke Nidhi Chauhan Development of Nanomaterial-Modified Impedimetric Aptasensor—A Single-Step Strategy for 3,4-Methylenedioxymethylamphetamine Detection Biosensors 3,4-methylenedioxymethylamphetamine electrochemical sensor aptamer tin nanoparticles recreational drugs forensic |
| title | Development of Nanomaterial-Modified Impedimetric Aptasensor—A Single-Step Strategy for 3,4-Methylenedioxymethylamphetamine Detection |
| title_full | Development of Nanomaterial-Modified Impedimetric Aptasensor—A Single-Step Strategy for 3,4-Methylenedioxymethylamphetamine Detection |
| title_fullStr | Development of Nanomaterial-Modified Impedimetric Aptasensor—A Single-Step Strategy for 3,4-Methylenedioxymethylamphetamine Detection |
| title_full_unstemmed | Development of Nanomaterial-Modified Impedimetric Aptasensor—A Single-Step Strategy for 3,4-Methylenedioxymethylamphetamine Detection |
| title_short | Development of Nanomaterial-Modified Impedimetric Aptasensor—A Single-Step Strategy for 3,4-Methylenedioxymethylamphetamine Detection |
| title_sort | development of nanomaterial modified impedimetric aptasensor a single step strategy for 3 4 methylenedioxymethylamphetamine detection |
| topic | 3,4-methylenedioxymethylamphetamine electrochemical sensor aptamer tin nanoparticles recreational drugs forensic |
| url | https://www.mdpi.com/2079-6374/12/7/538 |
| work_keys_str_mv | AT shringikasoni developmentofnanomaterialmodifiedimpedimetricaptasensorasinglestepstrategyfor34methylenedioxymethylamphetaminedetection AT utkarshjain developmentofnanomaterialmodifiedimpedimetricaptasensorasinglestepstrategyfor34methylenedioxymethylamphetaminedetection AT donaldhburke developmentofnanomaterialmodifiedimpedimetricaptasensorasinglestepstrategyfor34methylenedioxymethylamphetaminedetection AT nidhichauhan developmentofnanomaterialmodifiedimpedimetricaptasensorasinglestepstrategyfor34methylenedioxymethylamphetaminedetection |