Isolation of pathogenic strains of methicillin-resistant Staphylococcus aureus from mobile phones in a tertiary institution in Ibadan, Nigeria
The health implications of mobile phones emission have been a matter of concern to health practitioners for decades but little or no attention has been given to the mobile phones as potential fomites for pathogens. This study isolated and characterised methicillin-resistant Staphylococcus aureus (MR...
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| Format: | Article |
| Language: | English |
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Nigerian Academy of Science
2022-12-01
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| Series: | The Proceedings of the Nigerian Academy of Science |
| Subjects: | |
| Online Access: | https://nasjournal.org.ng/site/index.php/pnas/article/view/467/217 |
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| author | Olumuyiwa S. Alabi Oluwatobi O. Ayoola Jesutofunmi S. Odeyemi Anderson O. Oaikhena |
| author_facet | Olumuyiwa S. Alabi Oluwatobi O. Ayoola Jesutofunmi S. Odeyemi Anderson O. Oaikhena |
| author_sort | Olumuyiwa S. Alabi |
| collection | DOAJ |
| description | The health implications of mobile phones emission have been a matter of concern to health practitioners for decades but little or no attention has been given to the mobile phones as potential fomites for pathogens. This study isolated and characterised methicillin-resistant Staphylococcus aureus (MRSA) on mobile phones of students in a tertiary institution in Ibadan, Nigeria. One hundred swab samples were obtained from the surfaces of mobile phones of students in a cross-sectional study conducted between July and August, 2019. Presumptive staphylococci were isolated on mannitol salt agar and identities were confirmed by standard biochemical test and Gram-staining. Antimicrobial susceptibility test was done by disc-diffusion method and mecA or mecALGA251, tsst-1, eta and etb genes screened by PCR technique. Among the 200 presumptive S. aureus isolated, 163 (81.5%) were confirmed as S. aureus exhibiting resistance to ofloxacin (1.8%), gentamicin (3.7%), ceftriaxone (29.4%), ceftazidime (57.7%), cefoxitin (61.3%), cefuroxime (64.4%), erythromycin (85.3%), amoxicillin-clavulanic acid (86.5%) and cloxacillin (92%). Proportion of MRSA and methicillin-susceptible S. aureus (MSSA) were 61.3% and 38.7% respectively with significant difference in their level of antibiotic resistance to the cephalosporins (excluding ceftriaxone), penicillins, erythromycin and ofloxacin (p<0.05). All the MRSA strains were multidrug resistant (MDR) harbouring mecA or mecALGA251 (56.3%), tsst-1 (56.3%), etb (31.3%) and eta (12.5%) genes. This study detected a high proportion of methicillin-resistant, virulent S. aureus as fomites on surfaces of mobile phones among students of a tertiary institution. This is of public health concern and thus requires strong public education to avoid imminent spread and outbreaks. |
| first_indexed | 2024-03-13T04:19:47Z |
| format | Article |
| id | doaj.art-79f730bf09224fb9a080df977b26f472 |
| institution | Directory Open Access Journal |
| issn | 0794-7976 2705-327X |
| language | English |
| last_indexed | 2024-03-13T04:19:47Z |
| publishDate | 2022-12-01 |
| publisher | Nigerian Academy of Science |
| record_format | Article |
| series | The Proceedings of the Nigerian Academy of Science |
| spelling | doaj.art-79f730bf09224fb9a080df977b26f4722023-06-20T11:23:55ZengNigerian Academy of ScienceThe Proceedings of the Nigerian Academy of Science0794-79762705-327X2022-12-011526377https://doi.org/10.57046/KVHM5191Isolation of pathogenic strains of methicillin-resistant Staphylococcus aureus from mobile phones in a tertiary institution in Ibadan, NigeriaOlumuyiwa S. Alabi0Oluwatobi O. Ayoola1Jesutofunmi S. Odeyemi2Anderson O. Oaikhena3Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Oyo State, NigeriaDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Oyo State, NigeriaDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Oyo State, NigeriaDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Oyo State, NigeriaThe health implications of mobile phones emission have been a matter of concern to health practitioners for decades but little or no attention has been given to the mobile phones as potential fomites for pathogens. This study isolated and characterised methicillin-resistant Staphylococcus aureus (MRSA) on mobile phones of students in a tertiary institution in Ibadan, Nigeria. One hundred swab samples were obtained from the surfaces of mobile phones of students in a cross-sectional study conducted between July and August, 2019. Presumptive staphylococci were isolated on mannitol salt agar and identities were confirmed by standard biochemical test and Gram-staining. Antimicrobial susceptibility test was done by disc-diffusion method and mecA or mecALGA251, tsst-1, eta and etb genes screened by PCR technique. Among the 200 presumptive S. aureus isolated, 163 (81.5%) were confirmed as S. aureus exhibiting resistance to ofloxacin (1.8%), gentamicin (3.7%), ceftriaxone (29.4%), ceftazidime (57.7%), cefoxitin (61.3%), cefuroxime (64.4%), erythromycin (85.3%), amoxicillin-clavulanic acid (86.5%) and cloxacillin (92%). Proportion of MRSA and methicillin-susceptible S. aureus (MSSA) were 61.3% and 38.7% respectively with significant difference in their level of antibiotic resistance to the cephalosporins (excluding ceftriaxone), penicillins, erythromycin and ofloxacin (p<0.05). All the MRSA strains were multidrug resistant (MDR) harbouring mecA or mecALGA251 (56.3%), tsst-1 (56.3%), etb (31.3%) and eta (12.5%) genes. This study detected a high proportion of methicillin-resistant, virulent S. aureus as fomites on surfaces of mobile phones among students of a tertiary institution. This is of public health concern and thus requires strong public education to avoid imminent spread and outbreaks.https://nasjournal.org.ng/site/index.php/pnas/article/view/467/217mrsamobile phoneetaetbtsst-1mecamecalga251 |
| spellingShingle | Olumuyiwa S. Alabi Oluwatobi O. Ayoola Jesutofunmi S. Odeyemi Anderson O. Oaikhena Isolation of pathogenic strains of methicillin-resistant Staphylococcus aureus from mobile phones in a tertiary institution in Ibadan, Nigeria The Proceedings of the Nigerian Academy of Science mrsa mobile phone eta etb tsst-1 meca mecalga251 |
| title | Isolation of pathogenic strains of methicillin-resistant Staphylococcus aureus from mobile phones in a tertiary institution in Ibadan, Nigeria |
| title_full | Isolation of pathogenic strains of methicillin-resistant Staphylococcus aureus from mobile phones in a tertiary institution in Ibadan, Nigeria |
| title_fullStr | Isolation of pathogenic strains of methicillin-resistant Staphylococcus aureus from mobile phones in a tertiary institution in Ibadan, Nigeria |
| title_full_unstemmed | Isolation of pathogenic strains of methicillin-resistant Staphylococcus aureus from mobile phones in a tertiary institution in Ibadan, Nigeria |
| title_short | Isolation of pathogenic strains of methicillin-resistant Staphylococcus aureus from mobile phones in a tertiary institution in Ibadan, Nigeria |
| title_sort | isolation of pathogenic strains of methicillin resistant staphylococcus aureus from mobile phones in a tertiary institution in ibadan nigeria |
| topic | mrsa mobile phone eta etb tsst-1 meca mecalga251 |
| url | https://nasjournal.org.ng/site/index.php/pnas/article/view/467/217 |
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