Integrative Approach to <i>Phlebotomus mascittii</i> Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights
Sand flies (Diptera: Psychodidae: Phlebotominae) are blood-feeding insects that transmit the protozoan parasites <i>Leishmania</i> spp. and various arthropod-borne (arbo) viruses. While in Mediterranean parts of Europe the sand fly fauna is diverse, in Central European countries includin...
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MDPI AG
2020-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/12/1032 |
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| author | Edwin Kniha Vít Dvořák Petr Halada Markus Milchram Adelheid G. Obwaller Katrin Kuhls Susanne Schlegel Martina Köhsler Wolfgang Poeppl Karin Bakran-Lebl Hans-Peter Fuehrer Věra Volfová Gerhard Mooseder Vladimir Ivovic Petr Volf Julia Walochnik |
| author_facet | Edwin Kniha Vít Dvořák Petr Halada Markus Milchram Adelheid G. Obwaller Katrin Kuhls Susanne Schlegel Martina Köhsler Wolfgang Poeppl Karin Bakran-Lebl Hans-Peter Fuehrer Věra Volfová Gerhard Mooseder Vladimir Ivovic Petr Volf Julia Walochnik |
| author_sort | Edwin Kniha |
| collection | DOAJ |
| description | Sand flies (Diptera: Psychodidae: Phlebotominae) are blood-feeding insects that transmit the protozoan parasites <i>Leishmania</i> spp. and various arthropod-borne (arbo) viruses. While in Mediterranean parts of Europe the sand fly fauna is diverse, in Central European countries including Austria mainly <i>Phlebotomus mascittii</i> is found, an assumed but unproven vector of <i>Leishmania infantum</i>. To update the currently understudied sand fly distribution in Austria, a sand fly survey was performed and other entomological catches were screened for sand flies. Seven new trapping locations of <i>Ph. mascittii</i> are reported including the first record in Vienna, representing also one of the first findings of this species in a city. Morphological identification, supported by fluorescence microscopy, was confirmed by two molecular approaches, including sequencing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) protein profiling. Sand fly occurrence and activity were evaluated based on surveyed locations, habitat requirements and climatic parameters. Moreover, a first comparison of European <i>Ph. mascittii</i> populations was made by two marker genes, cytochrome c oxidase subunit 1 (<i>COI</i>), and cytochrome b (<i>cytb</i>), as well as MALDI-TOF mass spectra. Our study provides new important records of <i>Ph. mascittii</i> in Austria and valuable data for prospective entomological surveys. MALDI-TOF MS protein profiling was shown to be a reliable tool for differentiation between sand fly species. Rising temperatures and globalization demand for regular entomological surveys to monitor changes in species distribution and composition. This is also important with respect to the possible vector competence of <i>Ph. mascittii</i>. |
| first_indexed | 2024-03-10T14:12:45Z |
| format | Article |
| id | doaj.art-79f9b9cbd7174e6d969caf49954b2612 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T14:12:45Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-79f9b9cbd7174e6d969caf49954b26122023-11-21T00:03:10ZengMDPI AGPathogens2076-08172020-12-01912103210.3390/pathogens9121032Integrative Approach to <i>Phlebotomus mascittii</i> Grassi, 1908: First Record in Vienna with New Morphological and Molecular InsightsEdwin Kniha0Vít Dvořák1Petr Halada2Markus Milchram3Adelheid G. Obwaller4Katrin Kuhls5Susanne Schlegel6Martina Köhsler7Wolfgang Poeppl8Karin Bakran-Lebl9Hans-Peter Fuehrer10Věra Volfová11Gerhard Mooseder12Vladimir Ivovic13Petr Volf14Julia Walochnik15Institute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Parasitology, Faculty of Science, Charles University Prague, 128 43 Prague, Czech RepublicBioCeV, Institute of Microbiology of the Czech Academy of Sciences, 252 50 Vestec, Czech RepublicDepartment of Integrative Biology and Biodiversity Research, Institute of Zoology, University of Natural Resources and Life Sciences Vienna, 1180 Vienna, AustriaFederal Ministry of Defence, Division of Science, Research and Development, 1090 Vienna, AustriaDivision Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, GermanyDivision Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, GermanyInstitute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Dermatology and Tropical Medicine, Military Medical Cluster East, Austrian Armed Forces, 1210 Vienna, AustriaDepartment of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, 1210 Vienna, AustriaDepartment of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, 1210 Vienna, AustriaDepartment of Parasitology, Faculty of Science, Charles University Prague, 128 43 Prague, Czech RepublicDepartment of Dermatology and Tropical Medicine, Military Medical Cluster East, Austrian Armed Forces, 1210 Vienna, AustriaDepartment of Biodiversity, FAMNIT, University of Primorska, 6000 Koper-Capodistria, SloveniaDepartment of Parasitology, Faculty of Science, Charles University Prague, 128 43 Prague, Czech RepublicInstitute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaSand flies (Diptera: Psychodidae: Phlebotominae) are blood-feeding insects that transmit the protozoan parasites <i>Leishmania</i> spp. and various arthropod-borne (arbo) viruses. While in Mediterranean parts of Europe the sand fly fauna is diverse, in Central European countries including Austria mainly <i>Phlebotomus mascittii</i> is found, an assumed but unproven vector of <i>Leishmania infantum</i>. To update the currently understudied sand fly distribution in Austria, a sand fly survey was performed and other entomological catches were screened for sand flies. Seven new trapping locations of <i>Ph. mascittii</i> are reported including the first record in Vienna, representing also one of the first findings of this species in a city. Morphological identification, supported by fluorescence microscopy, was confirmed by two molecular approaches, including sequencing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) protein profiling. Sand fly occurrence and activity were evaluated based on surveyed locations, habitat requirements and climatic parameters. Moreover, a first comparison of European <i>Ph. mascittii</i> populations was made by two marker genes, cytochrome c oxidase subunit 1 (<i>COI</i>), and cytochrome b (<i>cytb</i>), as well as MALDI-TOF mass spectra. Our study provides new important records of <i>Ph. mascittii</i> in Austria and valuable data for prospective entomological surveys. MALDI-TOF MS protein profiling was shown to be a reliable tool for differentiation between sand fly species. Rising temperatures and globalization demand for regular entomological surveys to monitor changes in species distribution and composition. This is also important with respect to the possible vector competence of <i>Ph. mascittii</i>.https://www.mdpi.com/2076-0817/9/12/1032<i>Transphlebotomus</i>Central EuropeautoimmunofluorescenceMALDI-TOF mass spectrometrygenotypingleishmaniasis |
| spellingShingle | Edwin Kniha Vít Dvořák Petr Halada Markus Milchram Adelheid G. Obwaller Katrin Kuhls Susanne Schlegel Martina Köhsler Wolfgang Poeppl Karin Bakran-Lebl Hans-Peter Fuehrer Věra Volfová Gerhard Mooseder Vladimir Ivovic Petr Volf Julia Walochnik Integrative Approach to <i>Phlebotomus mascittii</i> Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights Pathogens <i>Transphlebotomus</i> Central Europe autoimmunofluorescence MALDI-TOF mass spectrometry genotyping leishmaniasis |
| title | Integrative Approach to <i>Phlebotomus mascittii</i> Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights |
| title_full | Integrative Approach to <i>Phlebotomus mascittii</i> Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights |
| title_fullStr | Integrative Approach to <i>Phlebotomus mascittii</i> Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights |
| title_full_unstemmed | Integrative Approach to <i>Phlebotomus mascittii</i> Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights |
| title_short | Integrative Approach to <i>Phlebotomus mascittii</i> Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights |
| title_sort | integrative approach to i phlebotomus mascittii i grassi 1908 first record in vienna with new morphological and molecular insights |
| topic | <i>Transphlebotomus</i> Central Europe autoimmunofluorescence MALDI-TOF mass spectrometry genotyping leishmaniasis |
| url | https://www.mdpi.com/2076-0817/9/12/1032 |
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