NDRG2 suppresses the proliferation of clear cell renal cell carcinoma cell A-498
<p>Abstract</p> <p>Background</p> <p>Recently, the anti-tumor activity of N-myc downstream-regulated gene 2 (NDRG2) was shown decreased expression in clear cell renal cell carcinoma (CCRCC), but the role of the down-expression of NDRG2 has not been described.</p>...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-07-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Online Access: | http://www.jeccr.com/content/29/1/103 |
| _version_ | 1818757905212506112 |
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| author | Ma Jian-Jun Liao Cheng-Gong Jiang Xue Zhao Hua-Dong Yao Li-Bo Bao Ting-Yi |
| author_facet | Ma Jian-Jun Liao Cheng-Gong Jiang Xue Zhao Hua-Dong Yao Li-Bo Bao Ting-Yi |
| author_sort | Ma Jian-Jun |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Recently, the anti-tumor activity of N-myc downstream-regulated gene 2 (NDRG2) was shown decreased expression in clear cell renal cell carcinoma (CCRCC), but the role of the down-expression of NDRG2 has not been described.</p> <p>Methods</p> <p>The NDRG2 recombinant adenovirus plasmid was constructed. The proliferation rate and NDRG2 expression of cell infected with recombinant plasmid were mesured by MTT, Flow cytometry analysis and western blot.</p> <p>Results</p> <p>The CCRCC cell A-498 re-expressed NDRG2 when infected by NDRG2 recombinant adenovirus and significantly decreased the proliferation rate. Fluorescence activated cell sorter analysis showed that 25.00% of cells expressed NDRG2 were in S-phase compared to 40.67% of control cells, whereas 62.08% of cells expressed NDRG2 were in G1-phase compared to 54.39% of control cells (<it>P </it>< 0.05). In addition, there were much more apoptotic cells in NDRG2-expressing cells than in the controls (<it>P </it>< 0.05). Moreover, upregulation of NDRG2 protein was associated with a reduction in cyclin D1, cyclin E, whereas cyclinD2, cyclinD3 and cdk2 were not affected examined by western blot. Furthermore, we found that p53 could upregulate NDRG2 expression in A-498 cell.</p> <p>Conclusions</p> <p>We found that NDRG2 can inhibit the proliferation of the renal carcinoma cells and induce arrest at G1 phase. p53 can up-regulate the expression of NDRG2. Our results showed that NDRG2 may function as a tumor suppressor in CCRCC.</p> |
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| format | Article |
| id | doaj.art-79fd2b79884946ef81ba0fea05b71c7a |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-18T06:18:21Z |
| publishDate | 2010-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-79fd2b79884946ef81ba0fea05b71c7a2022-12-21T21:18:11ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662010-07-0129110310.1186/1756-9966-29-103NDRG2 suppresses the proliferation of clear cell renal cell carcinoma cell A-498Ma Jian-JunLiao Cheng-GongJiang XueZhao Hua-DongYao Li-BoBao Ting-Yi<p>Abstract</p> <p>Background</p> <p>Recently, the anti-tumor activity of N-myc downstream-regulated gene 2 (NDRG2) was shown decreased expression in clear cell renal cell carcinoma (CCRCC), but the role of the down-expression of NDRG2 has not been described.</p> <p>Methods</p> <p>The NDRG2 recombinant adenovirus plasmid was constructed. The proliferation rate and NDRG2 expression of cell infected with recombinant plasmid were mesured by MTT, Flow cytometry analysis and western blot.</p> <p>Results</p> <p>The CCRCC cell A-498 re-expressed NDRG2 when infected by NDRG2 recombinant adenovirus and significantly decreased the proliferation rate. Fluorescence activated cell sorter analysis showed that 25.00% of cells expressed NDRG2 were in S-phase compared to 40.67% of control cells, whereas 62.08% of cells expressed NDRG2 were in G1-phase compared to 54.39% of control cells (<it>P </it>< 0.05). In addition, there were much more apoptotic cells in NDRG2-expressing cells than in the controls (<it>P </it>< 0.05). Moreover, upregulation of NDRG2 protein was associated with a reduction in cyclin D1, cyclin E, whereas cyclinD2, cyclinD3 and cdk2 were not affected examined by western blot. Furthermore, we found that p53 could upregulate NDRG2 expression in A-498 cell.</p> <p>Conclusions</p> <p>We found that NDRG2 can inhibit the proliferation of the renal carcinoma cells and induce arrest at G1 phase. p53 can up-regulate the expression of NDRG2. Our results showed that NDRG2 may function as a tumor suppressor in CCRCC.</p>http://www.jeccr.com/content/29/1/103 |
| spellingShingle | Ma Jian-Jun Liao Cheng-Gong Jiang Xue Zhao Hua-Dong Yao Li-Bo Bao Ting-Yi NDRG2 suppresses the proliferation of clear cell renal cell carcinoma cell A-498 Journal of Experimental & Clinical Cancer Research |
| title | NDRG2 suppresses the proliferation of clear cell renal cell carcinoma cell A-498 |
| title_full | NDRG2 suppresses the proliferation of clear cell renal cell carcinoma cell A-498 |
| title_fullStr | NDRG2 suppresses the proliferation of clear cell renal cell carcinoma cell A-498 |
| title_full_unstemmed | NDRG2 suppresses the proliferation of clear cell renal cell carcinoma cell A-498 |
| title_short | NDRG2 suppresses the proliferation of clear cell renal cell carcinoma cell A-498 |
| title_sort | ndrg2 suppresses the proliferation of clear cell renal cell carcinoma cell a 498 |
| url | http://www.jeccr.com/content/29/1/103 |
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