Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation
Chromosome segregation requires both the separation of sister chromatids and the sustained condensation of chromatids during anaphase. In yeast cells, cohesin is not only required for sister chromatid cohesion but also plays a major role determining the structure of individual chromatids in metaphas...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-10-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/80147 |
| _version_ | 1798029312414187520 |
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| author | Jonay Garcia-Luis Hélène Bordelet Agnès Thierry Romain Koszul Luis Aragon |
| author_facet | Jonay Garcia-Luis Hélène Bordelet Agnès Thierry Romain Koszul Luis Aragon |
| author_sort | Jonay Garcia-Luis |
| collection | DOAJ |
| description | Chromosome segregation requires both the separation of sister chromatids and the sustained condensation of chromatids during anaphase. In yeast cells, cohesin is not only required for sister chromatid cohesion but also plays a major role determining the structure of individual chromatids in metaphase. Separase cleavage is thought to remove all cohesin complexes from chromosomes to initiate anaphase. It is thus not clear how the length and organisation of segregating chromatids is maintained during anaphase in the absence of cohesin. Here, we show that degradation of cohesin at the anaphase onset causes aberrant chromatid segregation. Hi-C analysis on segregating chromatids demonstrates that cohesin depletion causes loss of intrachromatid organisation. Surprisingly, tobacco etch virus (TEV)-mediated cleavage of cohesin does not dramatically disrupt chromatid organisation in anaphase, explaining why bulk segregation is achieved. In addition, we identified a small pool of cohesin complexes bound to telophase chromosomes in wild-type cells and show that they play a role in the organisation of centromeric regions. Our data demonstrates that in yeast cells cohesin function is not over in metaphase, but extends to the anaphase period when chromatids are segregating. |
| first_indexed | 2024-04-11T19:22:17Z |
| format | Article |
| id | doaj.art-79ff806a1b13495fb450c4c4437f9c52 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T19:22:17Z |
| publishDate | 2022-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-79ff806a1b13495fb450c4c4437f9c522022-12-22T04:07:16ZengeLife Sciences Publications LtdeLife2050-084X2022-10-011110.7554/eLife.80147Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregationJonay Garcia-Luis0Hélène Bordelet1https://orcid.org/0000-0002-8190-2326Agnès Thierry2Romain Koszul3https://orcid.org/0000-0002-3086-1173Luis Aragon4https://orcid.org/0000-0003-0634-6742DNA motors Group, MRC London Institute of Medical Sciences, London, United KingdomInstitut Pasteur, CNRS UMR 3525, Université Paris Cité, Unité Régulation Spatiale des Génomes, Paris, FranceInstitut Pasteur, CNRS UMR 3525, Université Paris Cité, Unité Régulation Spatiale des Génomes, Paris, FranceInstitut Pasteur, CNRS UMR 3525, Université Paris Cité, Unité Régulation Spatiale des Génomes, Paris, FranceDNA motors Group, MRC London Institute of Medical Sciences, London, United KingdomChromosome segregation requires both the separation of sister chromatids and the sustained condensation of chromatids during anaphase. In yeast cells, cohesin is not only required for sister chromatid cohesion but also plays a major role determining the structure of individual chromatids in metaphase. Separase cleavage is thought to remove all cohesin complexes from chromosomes to initiate anaphase. It is thus not clear how the length and organisation of segregating chromatids is maintained during anaphase in the absence of cohesin. Here, we show that degradation of cohesin at the anaphase onset causes aberrant chromatid segregation. Hi-C analysis on segregating chromatids demonstrates that cohesin depletion causes loss of intrachromatid organisation. Surprisingly, tobacco etch virus (TEV)-mediated cleavage of cohesin does not dramatically disrupt chromatid organisation in anaphase, explaining why bulk segregation is achieved. In addition, we identified a small pool of cohesin complexes bound to telophase chromosomes in wild-type cells and show that they play a role in the organisation of centromeric regions. Our data demonstrates that in yeast cells cohesin function is not over in metaphase, but extends to the anaphase period when chromatids are segregating.https://elifesciences.org/articles/80147cohesinchromosome segregationchromosome condensation |
| spellingShingle | Jonay Garcia-Luis Hélène Bordelet Agnès Thierry Romain Koszul Luis Aragon Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation eLife cohesin chromosome segregation chromosome condensation |
| title | Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation |
| title_full | Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation |
| title_fullStr | Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation |
| title_full_unstemmed | Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation |
| title_short | Depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation |
| title_sort | depletion or cleavage of cohesin during anaphase differentially affects chromatin structure and segregation |
| topic | cohesin chromosome segregation chromosome condensation |
| url | https://elifesciences.org/articles/80147 |
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