Study on improving the stability of adsorption-encapsulation immobilized Laccase@ZIF-67
Laccase (Trametes versicolor) was immobilized onto/into zeolite imidazolate framework-67 (ZIF-67) for the first time, materials were performed by assisting one-pot synthesis strategy in aqueous solution at room temperature. The resulting laccase@ZIF-67 composite was characterized by powder X-ray dif...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X20307906 |
| _version_ | 1818645487276785664 |
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| author | Zhaobo Wang Dajun Ren Hongyan Yu Shan Jiang Shuqin Zhang Xiaoqing Zhang |
| author_facet | Zhaobo Wang Dajun Ren Hongyan Yu Shan Jiang Shuqin Zhang Xiaoqing Zhang |
| author_sort | Zhaobo Wang |
| collection | DOAJ |
| description | Laccase (Trametes versicolor) was immobilized onto/into zeolite imidazolate framework-67 (ZIF-67) for the first time, materials were performed by assisting one-pot synthesis strategy in aqueous solution at room temperature. The resulting laccase@ZIF-67 composite was characterized by powder X-ray diffraction (PXRD), fourier transform infrared (FT-IR) spectroscopies, field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray (EDX), thermal gravimetric analyses (TGA). The results showed that laccase could be well immobilized on ZIF-67 materials. Laccase@ZIF-67 was stable on storage stability and reuseablility, retaining 88 % (15 days at 4 °C) and 59 % (five reaction cycles) of residual enzyme activity. Laccase@ZIF-67 exhibited relatively stable activity at pH 3–5. In addition, the thermal deactivation kinetic studies of laccase@ZIF-67 showed a lower k value, higher t1/2 and ΔG values along with the enhancement of thermodynamic parameters than that of free laccase, and laccase@ZIF-67 had excellent level of thermostability. |
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| id | doaj.art-7a019217a1b84c489d2637bfdb8d738d |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-17T00:31:31Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
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| series | Biotechnology Reports |
| spelling | doaj.art-7a019217a1b84c489d2637bfdb8d738d2022-12-21T22:10:16ZengElsevierBiotechnology Reports2215-017X2020-12-0128e00553Study on improving the stability of adsorption-encapsulation immobilized Laccase@ZIF-67Zhaobo Wang0Dajun Ren1Hongyan Yu2Shan Jiang3Shuqin Zhang4Xiaoqing Zhang5College of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China; Hubei Key Laboratory for Efficient Utilization and Agglomeration of Metallurgic Mineral Resources, Wuhan University of Science and Technology, Wuhan, Hubei, 430081, ChinaCollege of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China; Hubei Key Laboratory for Efficient Utilization and Agglomeration of Metallurgic Mineral Resources, Wuhan University of Science and Technology, Wuhan, Hubei, 430081, China; Corresponding author at: College of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China.College of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China; Hubei Key Laboratory for Efficient Utilization and Agglomeration of Metallurgic Mineral Resources, Wuhan University of Science and Technology, Wuhan, Hubei, 430081, ChinaCollege of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China; Hubei Key Laboratory for Efficient Utilization and Agglomeration of Metallurgic Mineral Resources, Wuhan University of Science and Technology, Wuhan, Hubei, 430081, ChinaCollege of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China; Hubei Key Laboratory for Efficient Utilization and Agglomeration of Metallurgic Mineral Resources, Wuhan University of Science and Technology, Wuhan, Hubei, 430081, ChinaCollege of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China; Hubei Key Laboratory for Efficient Utilization and Agglomeration of Metallurgic Mineral Resources, Wuhan University of Science and Technology, Wuhan, Hubei, 430081, ChinaLaccase (Trametes versicolor) was immobilized onto/into zeolite imidazolate framework-67 (ZIF-67) for the first time, materials were performed by assisting one-pot synthesis strategy in aqueous solution at room temperature. The resulting laccase@ZIF-67 composite was characterized by powder X-ray diffraction (PXRD), fourier transform infrared (FT-IR) spectroscopies, field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray (EDX), thermal gravimetric analyses (TGA). The results showed that laccase could be well immobilized on ZIF-67 materials. Laccase@ZIF-67 was stable on storage stability and reuseablility, retaining 88 % (15 days at 4 °C) and 59 % (five reaction cycles) of residual enzyme activity. Laccase@ZIF-67 exhibited relatively stable activity at pH 3–5. In addition, the thermal deactivation kinetic studies of laccase@ZIF-67 showed a lower k value, higher t1/2 and ΔG values along with the enhancement of thermodynamic parameters than that of free laccase, and laccase@ZIF-67 had excellent level of thermostability.http://www.sciencedirect.com/science/article/pii/S2215017X20307906Zeolite imidazolate framework-67 (ZIF-67)LaccaseEnzyme immobilizationEnzyme activityStability |
| spellingShingle | Zhaobo Wang Dajun Ren Hongyan Yu Shan Jiang Shuqin Zhang Xiaoqing Zhang Study on improving the stability of adsorption-encapsulation immobilized Laccase@ZIF-67 Biotechnology Reports Zeolite imidazolate framework-67 (ZIF-67) Laccase Enzyme immobilization Enzyme activity Stability |
| title | Study on improving the stability of adsorption-encapsulation immobilized Laccase@ZIF-67 |
| title_full | Study on improving the stability of adsorption-encapsulation immobilized Laccase@ZIF-67 |
| title_fullStr | Study on improving the stability of adsorption-encapsulation immobilized Laccase@ZIF-67 |
| title_full_unstemmed | Study on improving the stability of adsorption-encapsulation immobilized Laccase@ZIF-67 |
| title_short | Study on improving the stability of adsorption-encapsulation immobilized Laccase@ZIF-67 |
| title_sort | study on improving the stability of adsorption encapsulation immobilized laccase zif 67 |
| topic | Zeolite imidazolate framework-67 (ZIF-67) Laccase Enzyme immobilization Enzyme activity Stability |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X20307906 |
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