Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method
In this study, the presence of Arcobacter spp. was investigated in the faeces of cattle, sheep, goats, dogs and cloacal swab samples of chickens using the 16S rDNA-RFLP method. The prevalence of Arcobacter in these species was found to be 13%, 12%, 16%, 4% and 33%, respectively. On the other hand, A...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2017-06-01
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| Series: | Veterinární Medicína |
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| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201706-0001_isolation-and-determination-of-antimicrobial-resistance-of-arcobacter-species-isolated-from-animal-faeces-in-th.php |
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| author | S. Yesilmen A. Vural M.E. Erkan I.H. Yildirim |
| author_facet | S. Yesilmen A. Vural M.E. Erkan I.H. Yildirim |
| author_sort | S. Yesilmen |
| collection | DOAJ |
| description | In this study, the presence of Arcobacter spp. was investigated in the faeces of cattle, sheep, goats, dogs and cloacal swab samples of chickens using the 16S rDNA-RFLP method. The prevalence of Arcobacter in these species was found to be 13%, 12%, 16%, 4% and 33%, respectively. On the other hand, Arcobacter spp. could not be isolated from rabbit faeces. A total of 78 (13%) Arcobacter spp. isolates were obtained from the 500 faecal samples and 100 cloacal swab samples examined in this study. From these 78 Arcobacter isolates, 24 (30.8%), 20 (25.6%), 11 (14.1%), 8 (10.7%), 4 (5.1%), 3 (3.9%) and 2 (2.6%) were identified by 16S rDNA-RLFP as A. cryaerophilus, A. butz- leri, A. skirrowii, A. cloacae, A. cibarius, A. halophilus, and A. nitrofigilis, respectively. All A. cryaerophilus (n = 24) isolates were found to be resistant to cloxacillin; all A. butzleri (n = 20) and A. skirrowii isolates were found to be resistant to penicillin/novobiocin, cefoperazone, tetracycline and cloxacillin. It was determined in this study that clinically healthy cattle, sheep, goats, dogs and chickens are reservoirs of Arcobacter spp. |
| first_indexed | 2024-04-10T07:58:39Z |
| format | Article |
| id | doaj.art-7a01de6b0793400b9597780740bd712d |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:58:39Z |
| publishDate | 2017-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-7a01de6b0793400b9597780740bd712d2023-02-23T03:50:40ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922017-06-0162630130710.17221/69/2016-VETMEDvet-201706-0001Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP methodS. YesilmenA. Vural0M.E. Erkan1I.H. Yildirim2Faculty of Veterinary Medicine, University of Dicle, Diyarbakir, TurkeyFaculty of Veterinary Medicine, University of Dicle, Diyarbakir, TurkeyFaculty of Veterinary Medicine, University of Dicle, Diyarbakir, TurkeyIn this study, the presence of Arcobacter spp. was investigated in the faeces of cattle, sheep, goats, dogs and cloacal swab samples of chickens using the 16S rDNA-RFLP method. The prevalence of Arcobacter in these species was found to be 13%, 12%, 16%, 4% and 33%, respectively. On the other hand, Arcobacter spp. could not be isolated from rabbit faeces. A total of 78 (13%) Arcobacter spp. isolates were obtained from the 500 faecal samples and 100 cloacal swab samples examined in this study. From these 78 Arcobacter isolates, 24 (30.8%), 20 (25.6%), 11 (14.1%), 8 (10.7%), 4 (5.1%), 3 (3.9%) and 2 (2.6%) were identified by 16S rDNA-RLFP as A. cryaerophilus, A. butz- leri, A. skirrowii, A. cloacae, A. cibarius, A. halophilus, and A. nitrofigilis, respectively. All A. cryaerophilus (n = 24) isolates were found to be resistant to cloxacillin; all A. butzleri (n = 20) and A. skirrowii isolates were found to be resistant to penicillin/novobiocin, cefoperazone, tetracycline and cloxacillin. It was determined in this study that clinically healthy cattle, sheep, goats, dogs and chickens are reservoirs of Arcobacter spp.https://vetmed.agriculturejournals.cz/artkey/vet-201706-0001_isolation-and-determination-of-antimicrobial-resistance-of-arcobacter-species-isolated-from-animal-faeces-in-th.phpcattlesheepgoatsdogschickensrabbitsantibiogram |
| spellingShingle | S. Yesilmen A. Vural M.E. Erkan I.H. Yildirim Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method Veterinární Medicína cattle sheep goats dogs chickens rabbits antibiogram |
| title | Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method |
| title_full | Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method |
| title_fullStr | Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method |
| title_full_unstemmed | Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method |
| title_short | Isolation and determination of antimicrobial resistance of Arcobacter species isolated from animal faeces in the Diyarbakir region of Turkey using the 16S rDNA-RFLP method |
| title_sort | isolation and determination of antimicrobial resistance of arcobacter species isolated from animal faeces in the diyarbakir region of turkey using the 16s rdna rflp method |
| topic | cattle sheep goats dogs chickens rabbits antibiogram |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201706-0001_isolation-and-determination-of-antimicrobial-resistance-of-arcobacter-species-isolated-from-animal-faeces-in-th.php |
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