How to approach heterogeneous protein expression for biotechnological use: An overview
Production of recombinant proteins in Escherichia coli expression systems has shown many advantages, as well as disadvantages, especially for biotechnological and other down-stream applications. The choice of an appropriate vector depends on the gene, to be cloned for purification procedures and oth...
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| Format: | Article |
| Language: | English |
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SciCell s.r.o.
2017-06-01
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| Series: | Nova Biotechnologica et Chimica |
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| Online Access: | http://www.degruyter.com/view/j/nbec.2017.16.issue-1/nbec-2017-0001/nbec-2017-0001.xml?format=INT |
| _version_ | 1828330778109411328 |
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| author | Jamrichová Daniela Tišáková Lenka Jarábková Veronika Godány Andrej |
| author_facet | Jamrichová Daniela Tišáková Lenka Jarábková Veronika Godány Andrej |
| author_sort | Jamrichová Daniela |
| collection | DOAJ |
| description | Production of recombinant proteins in Escherichia coli expression systems has shown many advantages, as well as disadvantages, especially for biotechnological and other down-stream applications. The choice of an appropriate vector depends on the gene, to be cloned for purification procedures and other analyses. Selection of a suitable production strain plays an important role in the preparation of recombinant proteins. The main criteria for the selection of the host organism are the properties of the recombinant produced protein, its subsequent use and the total amount desired. The most common problems in eukaryotic gene expression and recombinant proteins purification are, for instance, post-translational modifications, formation of disulphide bonds, or inclusion bodies. Obtaining a purified protein is a key step enabling further characterization of its role in the biological system. Moreover, methods of protein purification have been developed in parallel with the discovery of proteins and the need for their studies and applications. After protein purification, and also between the individual purification steps, it is necessary to test protein stability under different conditions over time. Shortly, all the essential points have been briefly discussed, which could be encountered during production and purification of a recombinant protein of interest, especially from eukaryotic source and expressed heterogeneously in prokaryotic production system. |
| first_indexed | 2024-04-13T20:43:36Z |
| format | Article |
| id | doaj.art-7a029ee2b78c41c49a00d82a1d9d712b |
| institution | Directory Open Access Journal |
| issn | 1338-6905 |
| language | English |
| last_indexed | 2024-04-13T20:43:36Z |
| publishDate | 2017-06-01 |
| publisher | SciCell s.r.o. |
| record_format | Article |
| series | Nova Biotechnologica et Chimica |
| spelling | doaj.art-7a029ee2b78c41c49a00d82a1d9d712b2022-12-22T02:30:45ZengSciCell s.r.o.Nova Biotechnologica et Chimica1338-69052017-06-0116111110.1515/nbec-2017-0001nbec-2017-0001How to approach heterogeneous protein expression for biotechnological use: An overviewJamrichová Daniela0Tišáková Lenka1Jarábková Veronika2Godány Andrej3Department of Biology, Faculty of Natural Sciences, University of SS. Cyril and Methodius in Trnava, Nám. J. Herdu 2, Trnava, SK-917 01, Slovak RepublicDepartment of Biology, Faculty of Natural Sciences, University of SS. Cyril and Methodius in Trnava, Nám. J. Herdu 2, Trnava, SK-917 01, Slovak RepublicDepartment of Biology, Faculty of Natural Sciences, University of SS. Cyril and Methodius in Trnava, Nám. J. Herdu 2, Trnava, SK-917 01, Slovak RepublicDepartment of Biology, Faculty of Natural Sciences, University of SS. Cyril and Methodius in Trnava, Nám. J. Herdu 2, Trnava, SK-917 01, Slovak RepublicProduction of recombinant proteins in Escherichia coli expression systems has shown many advantages, as well as disadvantages, especially for biotechnological and other down-stream applications. The choice of an appropriate vector depends on the gene, to be cloned for purification procedures and other analyses. Selection of a suitable production strain plays an important role in the preparation of recombinant proteins. The main criteria for the selection of the host organism are the properties of the recombinant produced protein, its subsequent use and the total amount desired. The most common problems in eukaryotic gene expression and recombinant proteins purification are, for instance, post-translational modifications, formation of disulphide bonds, or inclusion bodies. Obtaining a purified protein is a key step enabling further characterization of its role in the biological system. Moreover, methods of protein purification have been developed in parallel with the discovery of proteins and the need for their studies and applications. After protein purification, and also between the individual purification steps, it is necessary to test protein stability under different conditions over time. Shortly, all the essential points have been briefly discussed, which could be encountered during production and purification of a recombinant protein of interest, especially from eukaryotic source and expressed heterogeneously in prokaryotic production system.http://www.degruyter.com/view/j/nbec.2017.16.issue-1/nbec-2017-0001/nbec-2017-0001.xml?format=INTEscherichia coli Gene expression Protein purification Heterologous |
| spellingShingle | Jamrichová Daniela Tišáková Lenka Jarábková Veronika Godány Andrej How to approach heterogeneous protein expression for biotechnological use: An overview Nova Biotechnologica et Chimica Escherichia coli Gene expression Protein purification Heterologous |
| title | How to approach heterogeneous protein expression for biotechnological use: An overview |
| title_full | How to approach heterogeneous protein expression for biotechnological use: An overview |
| title_fullStr | How to approach heterogeneous protein expression for biotechnological use: An overview |
| title_full_unstemmed | How to approach heterogeneous protein expression for biotechnological use: An overview |
| title_short | How to approach heterogeneous protein expression for biotechnological use: An overview |
| title_sort | how to approach heterogeneous protein expression for biotechnological use an overview |
| topic | Escherichia coli Gene expression Protein purification Heterologous |
| url | http://www.degruyter.com/view/j/nbec.2017.16.issue-1/nbec-2017-0001/nbec-2017-0001.xml?format=INT |
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