Dietary Supplementation with Puerarin Improves Intestinal Function in Piglets Challenged with <i>Escherichia coli</i> K88
The objective of this study was to investigate the effect of puerarin supplementation on the growth performance and intestinal function of piglets challenged with enterotoxigenic <i>Escherichia coli</i> (ETEC) K88. Twenty-four ternary crossbred piglets were randomly assigned to three tre...
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MDPI AG
2023-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/12/1908 |
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| author | Yitong Zeng Rui Li Yi Dong Dan Yi Tao Wu Lei Wang Di Zhao Yanyan Zhang Yongqing Hou |
| author_facet | Yitong Zeng Rui Li Yi Dong Dan Yi Tao Wu Lei Wang Di Zhao Yanyan Zhang Yongqing Hou |
| author_sort | Yitong Zeng |
| collection | DOAJ |
| description | The objective of this study was to investigate the effect of puerarin supplementation on the growth performance and intestinal function of piglets challenged with enterotoxigenic <i>Escherichia coli</i> (ETEC) K88. Twenty-four ternary crossbred piglets were randomly assigned to three treatment groups: control group, ETEC group (challenged with ETEC K88 on day 8), and ETEC + Puerarin group (supplemented with 5 mg/kg puerarin and challenged with ETEC K88 on day 8). All piglets were orally administered D-xylose (0.1 g/kg body weight) on day 10, and blood samples were collected after 1 h. Subsequently, piglets were killed and intestinal samples were collected for further analysis. The results showed that puerarin supplementation significantly decreased the adverse effects of ETEC K88-challenged piglets; significantly improved growth performance; increased the number of <i>Bifidobacterium</i> in the colon and <i>Lactobacillus</i> in the jejunum, cecum and colon; decreased the number of <i>Escherichia coli</i> in the jejunum and cecum; reduced the hydrogen peroxide content in the jejunum and myeloperoxidase activity in the jejunum and ileum; and increased the activities of catalase and superoxide dismutase in the jejunum and ileum. In addition, puerarin supplementation alleviated ETEC K88-induced intestinal injury in piglets, significantly downregulated the mRNA level of Interleukin-1β and upregulated the mRNA levels of intercellular cell adhesion molecule-1, myxovirus resistance protein 1, myxovirus resistance protein 2, and guanylate-binding protein-1 in the small intestine of piglets. In conclusion, dietary supplementation with puerarin could attenuate ETEC K88-induced intestinal injury by increasing the antioxidant and anti-inflammatory capacity and the number of beneficial intestinal bacteria in piglets. |
| first_indexed | 2024-03-11T02:51:49Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T02:51:49Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-7a02ebed7edc43c3ad13aa475458fc832023-11-18T08:57:54ZengMDPI AGAnimals2076-26152023-06-011312190810.3390/ani13121908Dietary Supplementation with Puerarin Improves Intestinal Function in Piglets Challenged with <i>Escherichia coli</i> K88Yitong Zeng0Rui Li1Yi Dong2Dan Yi3Tao Wu4Lei Wang5Di Zhao6Yanyan Zhang7Yongqing Hou8Engineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, ChinaEngineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, ChinaEngineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, ChinaEngineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, ChinaEngineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, ChinaEngineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, ChinaEngineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, ChinaEngineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, ChinaEngineering Research Center of Feed Protein Resources on Agricultural By-Products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, ChinaThe objective of this study was to investigate the effect of puerarin supplementation on the growth performance and intestinal function of piglets challenged with enterotoxigenic <i>Escherichia coli</i> (ETEC) K88. Twenty-four ternary crossbred piglets were randomly assigned to three treatment groups: control group, ETEC group (challenged with ETEC K88 on day 8), and ETEC + Puerarin group (supplemented with 5 mg/kg puerarin and challenged with ETEC K88 on day 8). All piglets were orally administered D-xylose (0.1 g/kg body weight) on day 10, and blood samples were collected after 1 h. Subsequently, piglets were killed and intestinal samples were collected for further analysis. The results showed that puerarin supplementation significantly decreased the adverse effects of ETEC K88-challenged piglets; significantly improved growth performance; increased the number of <i>Bifidobacterium</i> in the colon and <i>Lactobacillus</i> in the jejunum, cecum and colon; decreased the number of <i>Escherichia coli</i> in the jejunum and cecum; reduced the hydrogen peroxide content in the jejunum and myeloperoxidase activity in the jejunum and ileum; and increased the activities of catalase and superoxide dismutase in the jejunum and ileum. In addition, puerarin supplementation alleviated ETEC K88-induced intestinal injury in piglets, significantly downregulated the mRNA level of Interleukin-1β and upregulated the mRNA levels of intercellular cell adhesion molecule-1, myxovirus resistance protein 1, myxovirus resistance protein 2, and guanylate-binding protein-1 in the small intestine of piglets. In conclusion, dietary supplementation with puerarin could attenuate ETEC K88-induced intestinal injury by increasing the antioxidant and anti-inflammatory capacity and the number of beneficial intestinal bacteria in piglets.https://www.mdpi.com/2076-2615/13/12/1908pigletspuerarinenterotoxigenic <i>Escherichia coli</i>growth performanceintestinal function |
| spellingShingle | Yitong Zeng Rui Li Yi Dong Dan Yi Tao Wu Lei Wang Di Zhao Yanyan Zhang Yongqing Hou Dietary Supplementation with Puerarin Improves Intestinal Function in Piglets Challenged with <i>Escherichia coli</i> K88 Animals piglets puerarin enterotoxigenic <i>Escherichia coli</i> growth performance intestinal function |
| title | Dietary Supplementation with Puerarin Improves Intestinal Function in Piglets Challenged with <i>Escherichia coli</i> K88 |
| title_full | Dietary Supplementation with Puerarin Improves Intestinal Function in Piglets Challenged with <i>Escherichia coli</i> K88 |
| title_fullStr | Dietary Supplementation with Puerarin Improves Intestinal Function in Piglets Challenged with <i>Escherichia coli</i> K88 |
| title_full_unstemmed | Dietary Supplementation with Puerarin Improves Intestinal Function in Piglets Challenged with <i>Escherichia coli</i> K88 |
| title_short | Dietary Supplementation with Puerarin Improves Intestinal Function in Piglets Challenged with <i>Escherichia coli</i> K88 |
| title_sort | dietary supplementation with puerarin improves intestinal function in piglets challenged with i escherichia coli i k88 |
| topic | piglets puerarin enterotoxigenic <i>Escherichia coli</i> growth performance intestinal function |
| url | https://www.mdpi.com/2076-2615/13/12/1908 |
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