The effect of Lactobacillus fermentum ME-3 on the intestinal microbiota and urine polyamines content: A double-blind placebo-controlled pilot trial
The aim of present study was to evaluate the effects of the probiotic Lactobacillus fermentum ME-3 on composition of gut microbiota and urine polyamines level. The healthy volunteers were divided into two groups: probiotic group received kefir with L. fermentum ME-3 and control group regular kefir b...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-09-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618301920 |
| _version_ | 1828933217225277440 |
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| author | Epp Sepp Imbi Smidt Jelena Štšepetova Tiiu Rööp Pirje Hütt Merle Rätsep Marika Mikelsaar |
| author_facet | Epp Sepp Imbi Smidt Jelena Štšepetova Tiiu Rööp Pirje Hütt Merle Rätsep Marika Mikelsaar |
| author_sort | Epp Sepp |
| collection | DOAJ |
| description | The aim of present study was to evaluate the effects of the probiotic Lactobacillus fermentum ME-3 on composition of gut microbiota and urine polyamines level. The healthy volunteers were divided into two groups: probiotic group received kefir with L. fermentum ME-3 and control group regular kefir both for 8 weeks. The lactoflora was determined by bacteriological method, gut microbiota by real-time PCR and urine polyamines level by gas-chromatography. In probiotic group there was an increase in the richness (p = 0.025), diversity (p = 0.017) and counts (p = 0.003) of lactoflora. That was associated with the increase of the diversity of the whole gut microbiota (p = 0.003). After probiotic consumption the level of urine putrescine had negative correlation with counts of lactobacilli (r = −0.424; p = 0.0083) and bifidobacteria (r = −0.415; p = 0.00983). L. fermentum ME-3 has potential to lower the risk of gut diseases that are bound with lower richness and diversity of gut microbiota and higher putrescine level. |
| first_indexed | 2024-12-14T01:12:53Z |
| format | Article |
| id | doaj.art-7a03e98810274ddba747f78262302b06 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T01:12:53Z |
| publishDate | 2018-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-7a03e98810274ddba747f78262302b062022-12-21T23:22:41ZengElsevierJournal of Functional Foods1756-46462018-09-0148430438The effect of Lactobacillus fermentum ME-3 on the intestinal microbiota and urine polyamines content: A double-blind placebo-controlled pilot trialEpp Sepp0Imbi Smidt1Jelena Štšepetova2Tiiu Rööp3Pirje Hütt4Merle Rätsep5Marika Mikelsaar6Corresponding author.; Department of Microbiology, University of Tartu, Ravila 19, 50411 Tartu, EstoniaDepartment of Microbiology, University of Tartu, Ravila 19, 50411 Tartu, EstoniaDepartment of Microbiology, University of Tartu, Ravila 19, 50411 Tartu, EstoniaDepartment of Microbiology, University of Tartu, Ravila 19, 50411 Tartu, EstoniaDepartment of Microbiology, University of Tartu, Ravila 19, 50411 Tartu, EstoniaDepartment of Microbiology, University of Tartu, Ravila 19, 50411 Tartu, EstoniaDepartment of Microbiology, University of Tartu, Ravila 19, 50411 Tartu, EstoniaThe aim of present study was to evaluate the effects of the probiotic Lactobacillus fermentum ME-3 on composition of gut microbiota and urine polyamines level. The healthy volunteers were divided into two groups: probiotic group received kefir with L. fermentum ME-3 and control group regular kefir both for 8 weeks. The lactoflora was determined by bacteriological method, gut microbiota by real-time PCR and urine polyamines level by gas-chromatography. In probiotic group there was an increase in the richness (p = 0.025), diversity (p = 0.017) and counts (p = 0.003) of lactoflora. That was associated with the increase of the diversity of the whole gut microbiota (p = 0.003). After probiotic consumption the level of urine putrescine had negative correlation with counts of lactobacilli (r = −0.424; p = 0.0083) and bifidobacteria (r = −0.415; p = 0.00983). L. fermentum ME-3 has potential to lower the risk of gut diseases that are bound with lower richness and diversity of gut microbiota and higher putrescine level.http://www.sciencedirect.com/science/article/pii/S1756464618301920ProbioticGut lactofloraGut microbesPolyamine |
| spellingShingle | Epp Sepp Imbi Smidt Jelena Štšepetova Tiiu Rööp Pirje Hütt Merle Rätsep Marika Mikelsaar The effect of Lactobacillus fermentum ME-3 on the intestinal microbiota and urine polyamines content: A double-blind placebo-controlled pilot trial Journal of Functional Foods Probiotic Gut lactoflora Gut microbes Polyamine |
| title | The effect of Lactobacillus fermentum ME-3 on the intestinal microbiota and urine polyamines content: A double-blind placebo-controlled pilot trial |
| title_full | The effect of Lactobacillus fermentum ME-3 on the intestinal microbiota and urine polyamines content: A double-blind placebo-controlled pilot trial |
| title_fullStr | The effect of Lactobacillus fermentum ME-3 on the intestinal microbiota and urine polyamines content: A double-blind placebo-controlled pilot trial |
| title_full_unstemmed | The effect of Lactobacillus fermentum ME-3 on the intestinal microbiota and urine polyamines content: A double-blind placebo-controlled pilot trial |
| title_short | The effect of Lactobacillus fermentum ME-3 on the intestinal microbiota and urine polyamines content: A double-blind placebo-controlled pilot trial |
| title_sort | effect of lactobacillus fermentum me 3 on the intestinal microbiota and urine polyamines content a double blind placebo controlled pilot trial |
| topic | Probiotic Gut lactoflora Gut microbes Polyamine |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618301920 |
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