Reduced glucose concentration enhances ultradian rhythms in Pdcd5 promoter activity in vitro
Intrinsically driven ultradian rhythms in the hourly range are often co-expressed with circadian rhythms in various physiological processes including metabolic processes such as feeding behaviour, gene expression and cellular metabolism. Several behavioural observations show that reduced energy inta...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-10-01
|
| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1244497/full |
| _version_ | 1797661516819857408 |
|---|---|
| author | Isaiah J. Ting Andreas Psomas Debra J. Skene Daan R. Van der Veen |
| author_facet | Isaiah J. Ting Andreas Psomas Debra J. Skene Daan R. Van der Veen |
| author_sort | Isaiah J. Ting |
| collection | DOAJ |
| description | Intrinsically driven ultradian rhythms in the hourly range are often co-expressed with circadian rhythms in various physiological processes including metabolic processes such as feeding behaviour, gene expression and cellular metabolism. Several behavioural observations show that reduced energy intake or increased energy expenditure leads to a re-balancing of ultradian and circadian timing, favouring ultradian feeding and activity patterns when energy availability is limited. This suggests a close link between ultradian rhythmicity and metabolic homeostasis, but we currently lack models to test this hypothesis at a cellular level. We therefore transduced 3T3-L1 pre-adipocyte cells with a reporter construct that drives a destabilised luciferase via the Pdcd5 promotor, a gene we previously showed to exhibit robust ultradian rhythms in vitro. Ultradian rhythmicity in Pdcd5 promotor driven bioluminescence was observed in >80% of all cultures that were synchronised with dexamethasone, whereas significantly lower numbers exhibited ultradian rhythmicity in non-synchronised cultures (∼11%). Cosine fits to ultradian bioluminescence rhythms in cells cultured and measured in low glucose concentrations (2 mM and 5 mM), exhibited significantly higher amplitudes than all other cultures, and a shorter period (6.9 h vs. 8.2 h, N = 12). Our findings show substantial ultradian rhythmicity in Pdcd5 promotor activity in cells in which the circadian clocks have been synchronised in vitro, which is in line with observations of circadian synchronisation of behavioural ultradian rhythms. Critically, we show that the amplitude of ultradian rhythms is enhanced in low glucose conditions, suggesting that low energy availability enhances ultradian rhythmicity at the cellular level in vitro. |
| first_indexed | 2024-03-11T18:46:48Z |
| format | Article |
| id | doaj.art-7a0401bad23241dca8b75b72360f758c |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-11T18:46:48Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-7a0401bad23241dca8b75b72360f758c2023-10-11T19:29:46ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-10-011410.3389/fphys.2023.12444971244497Reduced glucose concentration enhances ultradian rhythms in Pdcd5 promoter activity in vitroIsaiah J. TingAndreas PsomasDebra J. SkeneDaan R. Van der VeenIntrinsically driven ultradian rhythms in the hourly range are often co-expressed with circadian rhythms in various physiological processes including metabolic processes such as feeding behaviour, gene expression and cellular metabolism. Several behavioural observations show that reduced energy intake or increased energy expenditure leads to a re-balancing of ultradian and circadian timing, favouring ultradian feeding and activity patterns when energy availability is limited. This suggests a close link between ultradian rhythmicity and metabolic homeostasis, but we currently lack models to test this hypothesis at a cellular level. We therefore transduced 3T3-L1 pre-adipocyte cells with a reporter construct that drives a destabilised luciferase via the Pdcd5 promotor, a gene we previously showed to exhibit robust ultradian rhythms in vitro. Ultradian rhythmicity in Pdcd5 promotor driven bioluminescence was observed in >80% of all cultures that were synchronised with dexamethasone, whereas significantly lower numbers exhibited ultradian rhythmicity in non-synchronised cultures (∼11%). Cosine fits to ultradian bioluminescence rhythms in cells cultured and measured in low glucose concentrations (2 mM and 5 mM), exhibited significantly higher amplitudes than all other cultures, and a shorter period (6.9 h vs. 8.2 h, N = 12). Our findings show substantial ultradian rhythmicity in Pdcd5 promotor activity in cells in which the circadian clocks have been synchronised in vitro, which is in line with observations of circadian synchronisation of behavioural ultradian rhythms. Critically, we show that the amplitude of ultradian rhythms is enhanced in low glucose conditions, suggesting that low energy availability enhances ultradian rhythmicity at the cellular level in vitro.https://www.frontiersin.org/articles/10.3389/fphys.2023.1244497/fullultradiancircadianco-expressionadiposemetabolismPDCD5 |
| spellingShingle | Isaiah J. Ting Andreas Psomas Debra J. Skene Daan R. Van der Veen Reduced glucose concentration enhances ultradian rhythms in Pdcd5 promoter activity in vitro Frontiers in Physiology ultradian circadian co-expression adipose metabolism PDCD5 |
| title | Reduced glucose concentration enhances ultradian rhythms in Pdcd5 promoter activity in vitro |
| title_full | Reduced glucose concentration enhances ultradian rhythms in Pdcd5 promoter activity in vitro |
| title_fullStr | Reduced glucose concentration enhances ultradian rhythms in Pdcd5 promoter activity in vitro |
| title_full_unstemmed | Reduced glucose concentration enhances ultradian rhythms in Pdcd5 promoter activity in vitro |
| title_short | Reduced glucose concentration enhances ultradian rhythms in Pdcd5 promoter activity in vitro |
| title_sort | reduced glucose concentration enhances ultradian rhythms in pdcd5 promoter activity in vitro |
| topic | ultradian circadian co-expression adipose metabolism PDCD5 |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1244497/full |
| work_keys_str_mv | AT isaiahjting reducedglucoseconcentrationenhancesultradianrhythmsinpdcd5promoteractivityinvitro AT andreaspsomas reducedglucoseconcentrationenhancesultradianrhythmsinpdcd5promoteractivityinvitro AT debrajskene reducedglucoseconcentrationenhancesultradianrhythmsinpdcd5promoteractivityinvitro AT daanrvanderveen reducedglucoseconcentrationenhancesultradianrhythmsinpdcd5promoteractivityinvitro |