Cloning and Characterization of a Schistosoma mansoni cDNA Clone with a Specific Antigenic Expression during Development in a Vertebrate Host
Approximately 2.0 x 10 cDNA clones of an Schistosoma mansoni lgt11 cDNA library were screened in duplicate with serum from infected mice corresponding to distinct phases of infection. A cDNA clone (7/1) was isolated and recognized only by seven week serum. The clone was subcloned in pGEX-2T and West...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1997-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000500015 |
| _version_ | 1827844922975191040 |
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| author | Arlete Martins Coelho-Castelo Vanderlei Rodrigues |
| author_facet | Arlete Martins Coelho-Castelo Vanderlei Rodrigues |
| author_sort | Arlete Martins Coelho-Castelo |
| collection | DOAJ |
| description | Approximately 2.0 x 10 cDNA clones of an Schistosoma mansoni lgt11 cDNA library were screened in duplicate with serum from infected mice corresponding to distinct phases of infection. A cDNA clone (7/1) was isolated and recognized only by seven week serum. The clone was subcloned in pGEX-2T and Western-blot studies showed a specific antigenic expression confirming that only serum from the chronic phase is capable of recognizing this antigen. Dot-hybridization with RNA from different developmental phases of the parasite showed that the corresponding 7/1 RNA is expressed in all phases of parasite development in vertebrate hosts |
| first_indexed | 2024-03-12T08:49:58Z |
| format | Article |
| id | doaj.art-7a04c0e3ea124e7cb524fb3e0ad669c5 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:49:58Z |
| publishDate | 1997-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-7a04c0e3ea124e7cb524fb3e0ad669c52023-09-02T16:22:07ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601997-09-0192564310.1590/S0074-02761997000500015Cloning and Characterization of a Schistosoma mansoni cDNA Clone with a Specific Antigenic Expression during Development in a Vertebrate HostArlete Martins Coelho-CasteloVanderlei RodriguesApproximately 2.0 x 10 cDNA clones of an Schistosoma mansoni lgt11 cDNA library were screened in duplicate with serum from infected mice corresponding to distinct phases of infection. A cDNA clone (7/1) was isolated and recognized only by seven week serum. The clone was subcloned in pGEX-2T and Western-blot studies showed a specific antigenic expression confirming that only serum from the chronic phase is capable of recognizing this antigen. Dot-hybridization with RNA from different developmental phases of the parasite showed that the corresponding 7/1 RNA is expressed in all phases of parasite development in vertebrate hostshttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000500015Schistosoma mansoniantigen specific expressioncDNAcloning |
| spellingShingle | Arlete Martins Coelho-Castelo Vanderlei Rodrigues Cloning and Characterization of a Schistosoma mansoni cDNA Clone with a Specific Antigenic Expression during Development in a Vertebrate Host Memorias do Instituto Oswaldo Cruz Schistosoma mansoni antigen specific expression cDNA cloning |
| title | Cloning and Characterization of a Schistosoma mansoni cDNA Clone with a Specific Antigenic Expression during Development in a Vertebrate Host |
| title_full | Cloning and Characterization of a Schistosoma mansoni cDNA Clone with a Specific Antigenic Expression during Development in a Vertebrate Host |
| title_fullStr | Cloning and Characterization of a Schistosoma mansoni cDNA Clone with a Specific Antigenic Expression during Development in a Vertebrate Host |
| title_full_unstemmed | Cloning and Characterization of a Schistosoma mansoni cDNA Clone with a Specific Antigenic Expression during Development in a Vertebrate Host |
| title_short | Cloning and Characterization of a Schistosoma mansoni cDNA Clone with a Specific Antigenic Expression during Development in a Vertebrate Host |
| title_sort | cloning and characterization of a schistosoma mansoni cdna clone with a specific antigenic expression during development in a vertebrate host |
| topic | Schistosoma mansoni antigen specific expression cDNA cloning |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000500015 |
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