Dose-Response of Fruit Oligosaccharides on Rumen Fermentation Parameters, CH<sub>4</sub> Emission and Skatole Content In Vitro
The purpose of this work was to study the dose effects of fruit oligosaccharide (FOS) supplementation on rumen fermentation parameters, methane (CH<sub>4</sub>) production and skatole production. The rumen fluid of Hu sheep was collected through their fistula and immediately transferred...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/5/428 |
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| author | Liyan Wang Shoukun Ji Hui Yan Jinhui Li Lishen Zhang Dezhi Yan Chunhui Duan Yueqin Liu Yingjie Zhang |
| author_facet | Liyan Wang Shoukun Ji Hui Yan Jinhui Li Lishen Zhang Dezhi Yan Chunhui Duan Yueqin Liu Yingjie Zhang |
| author_sort | Liyan Wang |
| collection | DOAJ |
| description | The purpose of this work was to study the dose effects of fruit oligosaccharide (FOS) supplementation on rumen fermentation parameters, methane (CH<sub>4</sub>) production and skatole production. The rumen fluid of Hu sheep was collected through their fistula and immediately transferred to the laboratory for rumen fermentation in vitro. The experimental diet was supplemented with 0%, 0.2%, 0.8%, 1.2%, 1.8% and 2.4% FOS in the basal diet. Gas production (GP) and CH<sub>4</sub> production were measured and recorded at 2, 4, 6, 8, 10, 12, 24, 36 and 48 h. After 48 h of fermentation, degradation rates of nutritional components, fermentation parameters and skatole content were determined. The results showed that the GP, the nutrient degradation rates and the fermentation parameters of rumen linearly increased with increasing doses of FOS supplementation (<i>p</i> < 0.05). There was a quadratic trend between FOS addition and CH<sub>4</sub> production and skatole content in rumen fluid (<i>p</i> < 0.05). We also observed the CH<sub>4</sub> production in the 1.2% FOS-treated group was significantly lower than the other FOS-treated groups. Skatole content of the 0.2%, 0.8% and 1.2% FOS-treated groups were significantly lower than the other FOS-treated groups (<i>p</i> < 0.05). Our findings indicated that the effect of FOS on rumen fermentation parameters, CH<sub>4</sub> production and skatole production in vitro was dose-dependent. To improve the digestibility of nutrients and the fermentation parameters of rumen, a higher FOS dosage might be helpful. However, if CH<sub>4</sub> and skatole production is a concern, a dose of FOS at 1.2% is recommended. |
| first_indexed | 2024-03-11T03:45:47Z |
| format | Article |
| id | doaj.art-7a07344d43234bcb882055336d5d1e14 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T03:45:47Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-7a07344d43234bcb882055336d5d1e142023-11-18T01:17:40ZengMDPI AGFermentation2311-56372023-04-019542810.3390/fermentation9050428Dose-Response of Fruit Oligosaccharides on Rumen Fermentation Parameters, CH<sub>4</sub> Emission and Skatole Content In VitroLiyan Wang0Shoukun Ji1Hui Yan2Jinhui Li3Lishen Zhang4Dezhi Yan5Chunhui Duan6Yueqin Liu7Yingjie Zhang8College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaAgriculture and Rural Bureau of Tang County, Baoding 072350, ChinaShandong CROC Environmental Testing Co., Ltd., Jinan 250000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaThe purpose of this work was to study the dose effects of fruit oligosaccharide (FOS) supplementation on rumen fermentation parameters, methane (CH<sub>4</sub>) production and skatole production. The rumen fluid of Hu sheep was collected through their fistula and immediately transferred to the laboratory for rumen fermentation in vitro. The experimental diet was supplemented with 0%, 0.2%, 0.8%, 1.2%, 1.8% and 2.4% FOS in the basal diet. Gas production (GP) and CH<sub>4</sub> production were measured and recorded at 2, 4, 6, 8, 10, 12, 24, 36 and 48 h. After 48 h of fermentation, degradation rates of nutritional components, fermentation parameters and skatole content were determined. The results showed that the GP, the nutrient degradation rates and the fermentation parameters of rumen linearly increased with increasing doses of FOS supplementation (<i>p</i> < 0.05). There was a quadratic trend between FOS addition and CH<sub>4</sub> production and skatole content in rumen fluid (<i>p</i> < 0.05). We also observed the CH<sub>4</sub> production in the 1.2% FOS-treated group was significantly lower than the other FOS-treated groups. Skatole content of the 0.2%, 0.8% and 1.2% FOS-treated groups were significantly lower than the other FOS-treated groups (<i>p</i> < 0.05). Our findings indicated that the effect of FOS on rumen fermentation parameters, CH<sub>4</sub> production and skatole production in vitro was dose-dependent. To improve the digestibility of nutrients and the fermentation parameters of rumen, a higher FOS dosage might be helpful. However, if CH<sub>4</sub> and skatole production is a concern, a dose of FOS at 1.2% is recommended.https://www.mdpi.com/2311-5637/9/5/428fructo-oligosaccharidesskatolemethanerumen fermentationin vitro |
| spellingShingle | Liyan Wang Shoukun Ji Hui Yan Jinhui Li Lishen Zhang Dezhi Yan Chunhui Duan Yueqin Liu Yingjie Zhang Dose-Response of Fruit Oligosaccharides on Rumen Fermentation Parameters, CH<sub>4</sub> Emission and Skatole Content In Vitro Fermentation fructo-oligosaccharides skatole methane rumen fermentation in vitro |
| title | Dose-Response of Fruit Oligosaccharides on Rumen Fermentation Parameters, CH<sub>4</sub> Emission and Skatole Content In Vitro |
| title_full | Dose-Response of Fruit Oligosaccharides on Rumen Fermentation Parameters, CH<sub>4</sub> Emission and Skatole Content In Vitro |
| title_fullStr | Dose-Response of Fruit Oligosaccharides on Rumen Fermentation Parameters, CH<sub>4</sub> Emission and Skatole Content In Vitro |
| title_full_unstemmed | Dose-Response of Fruit Oligosaccharides on Rumen Fermentation Parameters, CH<sub>4</sub> Emission and Skatole Content In Vitro |
| title_short | Dose-Response of Fruit Oligosaccharides on Rumen Fermentation Parameters, CH<sub>4</sub> Emission and Skatole Content In Vitro |
| title_sort | dose response of fruit oligosaccharides on rumen fermentation parameters ch sub 4 sub emission and skatole content in vitro |
| topic | fructo-oligosaccharides skatole methane rumen fermentation in vitro |
| url | https://www.mdpi.com/2311-5637/9/5/428 |
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