LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung’s disease
Background: Hirschsprung’s disease (HSCR) is a common defect in newborns, and studies have revealed that long non-coding RNA (lncRNA) is involved in the progression of HSCR. This research study aims to investigate the mechanism of downregulated RNA in cancer (DRAIC) on cell proliferation and migrati...
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| Format: | Article |
| Language: | English |
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Upsala Medical Society
2021-08-01
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| Series: | Upsala Journal of Medical Sciences |
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| Online Access: | https://ujms.net/index.php/ujms/article/view/7895/13781 |
| _version_ | 1797721854548377600 |
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| author | Chuancheng Sun Bing Xu Liang Wang Yilin Su |
| author_facet | Chuancheng Sun Bing Xu Liang Wang Yilin Su |
| author_sort | Chuancheng Sun |
| collection | DOAJ |
| description | Background: Hirschsprung’s disease (HSCR) is a common defect in newborns, and studies have revealed that long non-coding RNA (lncRNA) is involved in the progression of HSCR. This research study aims to investigate the mechanism of downregulated RNA in cancer (DRAIC) on cell proliferation and migration in HSCR. Methods: Quantitative reverse transcription–polymerase chain reaction (qRT-PCR) was used to detect the expression of DRAIC in HSCR bowel stenosis tissues and normal colon tissues. Cell-counting kit-8 (CCK-8) and Transwell assays were employed to explore whether cellular functions change after overexpression or knockdown of the DRAIC in SH-SY5Y cells and human 293T cells. Protein expression levels were determined by Western blot analysis. RNA pull-down and dual-luciferase reporter assays were used to confirm the competitive relationship of DRAIC and integrin subunit alpha 6 (ITGA6) through their association with miR-34a-5p. Results: The lncRNA DRAIC was significantly increased in colon tissue from HSCR patients. The overexpression of DRAIC inhibited SH-SY5Y cell and human 293T cell proliferation and migration. Knockdown of DRAIC, however, promoted cell proliferation and migration. The RNA pull-down and dual-luciferase reporter assays have proven the competitive relationship between DRAIC and ITGA6 through their association with miR-34a-5p. Further rescue experiments have confirmed that DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in HSCR. Conclusion: DRAIC promoted cell proliferation and migration by regulating the miR-34a-5p/ITGA6 signal axis in HSCR. |
| first_indexed | 2024-03-12T09:39:12Z |
| format | Article |
| id | doaj.art-7a0a5002ab6f49709b905ad961c7f6ff |
| institution | Directory Open Access Journal |
| issn | 0300-9734 2000-1967 |
| language | English |
| last_indexed | 2024-03-12T09:39:12Z |
| publishDate | 2021-08-01 |
| publisher | Upsala Medical Society |
| record_format | Article |
| series | Upsala Journal of Medical Sciences |
| spelling | doaj.art-7a0a5002ab6f49709b905ad961c7f6ff2023-09-02T13:21:31ZengUpsala Medical SocietyUpsala Journal of Medical Sciences0300-97342000-19672021-08-011261810.48101/ujms.v126.78957895LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung’s diseaseChuancheng Sun0Bing Xu1Liang Wang2Yilin Su3Pediatric Surgery, The First Affiliated Hospital of China University of Science and Technology (Anhui Provincial Hospital), Hefei, Anhui, ChinaPediatric Surgery, The First Affiliated Hospital of China University of Science and Technology (Anhui Provincial Hospital), Hefei, Anhui, ChinaPediatric Surgery, The First Affiliated Hospital of China University of Science and Technology (Anhui Provincial Hospital), Hefei, Anhui, ChinaPediatric Surgery, The First Affiliated Hospital of China University of Science and Technology (Anhui Provincial Hospital), Hefei, Anhui, ChinaBackground: Hirschsprung’s disease (HSCR) is a common defect in newborns, and studies have revealed that long non-coding RNA (lncRNA) is involved in the progression of HSCR. This research study aims to investigate the mechanism of downregulated RNA in cancer (DRAIC) on cell proliferation and migration in HSCR. Methods: Quantitative reverse transcription–polymerase chain reaction (qRT-PCR) was used to detect the expression of DRAIC in HSCR bowel stenosis tissues and normal colon tissues. Cell-counting kit-8 (CCK-8) and Transwell assays were employed to explore whether cellular functions change after overexpression or knockdown of the DRAIC in SH-SY5Y cells and human 293T cells. Protein expression levels were determined by Western blot analysis. RNA pull-down and dual-luciferase reporter assays were used to confirm the competitive relationship of DRAIC and integrin subunit alpha 6 (ITGA6) through their association with miR-34a-5p. Results: The lncRNA DRAIC was significantly increased in colon tissue from HSCR patients. The overexpression of DRAIC inhibited SH-SY5Y cell and human 293T cell proliferation and migration. Knockdown of DRAIC, however, promoted cell proliferation and migration. The RNA pull-down and dual-luciferase reporter assays have proven the competitive relationship between DRAIC and ITGA6 through their association with miR-34a-5p. Further rescue experiments have confirmed that DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in HSCR. Conclusion: DRAIC promoted cell proliferation and migration by regulating the miR-34a-5p/ITGA6 signal axis in HSCR.https://ujms.net/index.php/ujms/article/view/7895/13781lncrna draicmir-34a-5pitga6hirschsprung’s diseaseproliferationmigration |
| spellingShingle | Chuancheng Sun Bing Xu Liang Wang Yilin Su LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung’s disease Upsala Journal of Medical Sciences lncrna draic mir-34a-5p itga6 hirschsprung’s disease proliferation migration |
| title | LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung’s disease |
| title_full | LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung’s disease |
| title_fullStr | LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung’s disease |
| title_full_unstemmed | LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung’s disease |
| title_short | LncRNA DRAIC regulates cell proliferation and migration by affecting the miR-34a-5p/ITGA6 signal axis in Hirschsprung’s disease |
| title_sort | lncrna draic regulates cell proliferation and migration by affecting the mir 34a 5p itga6 signal axis in hirschsprung s disease |
| topic | lncrna draic mir-34a-5p itga6 hirschsprung’s disease proliferation migration |
| url | https://ujms.net/index.php/ujms/article/view/7895/13781 |
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