Osteoblasts and Fibroblasts Interaction with a Porcine Acellular Dermal Matrix Membrane
The use of collagen membranes has remained the gold standard in GTR/GBR. In this study, the features and the biological activities of an acellular porcine dermis collagen matrix membrane applicable during dental surgery were investigated, and also by applying hydration with NaCl. Thus, two tested me...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/3649 |
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| author | Pietro Felice Emira D’Amico Tania Vanessa Pierfelice Morena Petrini Carlo Barausse Maryia Karaban Antonio Barone Giovanna Iezzi |
| author_facet | Pietro Felice Emira D’Amico Tania Vanessa Pierfelice Morena Petrini Carlo Barausse Maryia Karaban Antonio Barone Giovanna Iezzi |
| author_sort | Pietro Felice |
| collection | DOAJ |
| description | The use of collagen membranes has remained the gold standard in GTR/GBR. In this study, the features and the biological activities of an acellular porcine dermis collagen matrix membrane applicable during dental surgery were investigated, and also by applying hydration with NaCl. Thus, two tested membranes were distinguished, the H-Membrane and Membrane, compared to the control cell culture plastic. The characterization was performed by SEM and histological analyses. In contrast, the biocompatibility was investigated on HGF and HOB cells at 3, 7, and 14 days by MTT for proliferation study; by SEM and histology for cell interaction study; and by RT-PCR for function-related genes study. In HOBs seeded on membranes, mineralization functions by ALP assay and Alizarin Red staining were also investigated. Results indicated that the tested membranes, especially when hydrated, can promote the proliferation and attachment of cells at each time. Furthermore, membranes significantly increased ALP and mineralization activities in HOBs as well as the osteoblastic-related genes <i>ALP</i> and <i>OCN</i>. Similarly, membranes significantly increased ECM-related and <i>MMP8</i> gene expression in HGFs. In conclusion, the tested acellular porcine dermis collagen matrix membrane, mainly when it is hydrated, behaved as a suitable microenvironment for oral cells. |
| first_indexed | 2024-03-11T08:43:02Z |
| format | Article |
| id | doaj.art-7a0b66decfb844ca99268bc3b90ccf78 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:43:02Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7a0b66decfb844ca99268bc3b90ccf782023-11-16T21:03:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244364910.3390/ijms24043649Osteoblasts and Fibroblasts Interaction with a Porcine Acellular Dermal Matrix MembranePietro Felice0Emira D’Amico1Tania Vanessa Pierfelice2Morena Petrini3Carlo Barausse4Maryia Karaban5Antonio Barone6Giovanna Iezzi7Department of Biomedical and Neuromotor Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Biomedical and Neuromotor Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Biomedical and Neuromotor Sciences, University of Bologna, 40126 Bologna, ItalyUnit of Oral Surgery and Implantology, University Hospitals of Geneva, University of Geneva, 1205 Geneva, SwitzerlandDepartment of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyThe use of collagen membranes has remained the gold standard in GTR/GBR. In this study, the features and the biological activities of an acellular porcine dermis collagen matrix membrane applicable during dental surgery were investigated, and also by applying hydration with NaCl. Thus, two tested membranes were distinguished, the H-Membrane and Membrane, compared to the control cell culture plastic. The characterization was performed by SEM and histological analyses. In contrast, the biocompatibility was investigated on HGF and HOB cells at 3, 7, and 14 days by MTT for proliferation study; by SEM and histology for cell interaction study; and by RT-PCR for function-related genes study. In HOBs seeded on membranes, mineralization functions by ALP assay and Alizarin Red staining were also investigated. Results indicated that the tested membranes, especially when hydrated, can promote the proliferation and attachment of cells at each time. Furthermore, membranes significantly increased ALP and mineralization activities in HOBs as well as the osteoblastic-related genes <i>ALP</i> and <i>OCN</i>. Similarly, membranes significantly increased ECM-related and <i>MMP8</i> gene expression in HGFs. In conclusion, the tested acellular porcine dermis collagen matrix membrane, mainly when it is hydrated, behaved as a suitable microenvironment for oral cells.https://www.mdpi.com/1422-0067/24/4/3649dermal matrix membranecollagenhuman gingival fibroblastshuman oral osteoblastsALP<i>OCN</i> |
| spellingShingle | Pietro Felice Emira D’Amico Tania Vanessa Pierfelice Morena Petrini Carlo Barausse Maryia Karaban Antonio Barone Giovanna Iezzi Osteoblasts and Fibroblasts Interaction with a Porcine Acellular Dermal Matrix Membrane International Journal of Molecular Sciences dermal matrix membrane collagen human gingival fibroblasts human oral osteoblasts ALP <i>OCN</i> |
| title | Osteoblasts and Fibroblasts Interaction with a Porcine Acellular Dermal Matrix Membrane |
| title_full | Osteoblasts and Fibroblasts Interaction with a Porcine Acellular Dermal Matrix Membrane |
| title_fullStr | Osteoblasts and Fibroblasts Interaction with a Porcine Acellular Dermal Matrix Membrane |
| title_full_unstemmed | Osteoblasts and Fibroblasts Interaction with a Porcine Acellular Dermal Matrix Membrane |
| title_short | Osteoblasts and Fibroblasts Interaction with a Porcine Acellular Dermal Matrix Membrane |
| title_sort | osteoblasts and fibroblasts interaction with a porcine acellular dermal matrix membrane |
| topic | dermal matrix membrane collagen human gingival fibroblasts human oral osteoblasts ALP <i>OCN</i> |
| url | https://www.mdpi.com/1422-0067/24/4/3649 |
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