Phytochemical screening and antioxidant evaluation of millet varieties of Pakistan

The current research was planned to measure the comparative phytochemical and anti-oxidative potential of aqueous and methanolic extracts of two indigenously grown millet varieties of Pakistan. The locally available millet varieties, i.e. MB-87 and AF-POP flours were chemically characterized through...

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Main Authors: AMBREEN NAZ, ALLAH RUKHA, SHABBIR AHMAD, UMMARA WAHEED, KASHIF RAZZAQ, UMAR FAROOQ, NIGHAT RAZA, NOSHEEN NAZ
Format: Article
Language:English
Published: Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek 2020-01-01
Series:Croatian Journal of Food Science and Technology
Online Access:https://hrcak.srce.hr/file/346465
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author AMBREEN NAZ
ALLAH RUKHA
SHABBIR AHMAD
UMMARA WAHEED
KASHIF RAZZAQ
UMAR FAROOQ
NIGHAT RAZA
NOSHEEN NAZ
author_facet AMBREEN NAZ
ALLAH RUKHA
SHABBIR AHMAD
UMMARA WAHEED
KASHIF RAZZAQ
UMAR FAROOQ
NIGHAT RAZA
NOSHEEN NAZ
author_sort AMBREEN NAZ
collection DOAJ
description The current research was planned to measure the comparative phytochemical and anti-oxidative potential of aqueous and methanolic extracts of two indigenously grown millet varieties of Pakistan. The locally available millet varieties, i.e. MB-87 and AF-POP flours were chemically characterized through the proximate and mineral analysis. The antioxidant extract was prepared in water and methanol. The extracts were screened for saponins, terpenoids, flavonoids and tannins; methanolic extract of MB-87 and AF-POP showed flavonoids in an average quantity (++), whilst aqueous extract had only trace (+) values. The phytochemical screening showed the presence of saponins only in the aqueous extract of both varieties. However, terpenoids and tannins were present in both methanolic and aqueous extracts. During antioxidant evaluation, millet methanolic extracts showed higher level of TPC and DPPH of MB-87 and AF-POP as 0.30±0.001 & 0.23±0.0012 mg GAE/g and 48±0.96 &46±1.09%, respectively. However, the β-carotene activity of the aqueous extract of both varieties (MB-87 and AF-POP) was recorded higher. In a nutshell, the methanolic extract of MB-87 has an appreciable atioxidant profile. Furher research should be planned to screen the important constituents of Pakistani millet varieties.
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spelling doaj.art-7a0e56acf3dd42a98b4bc4ded712ca652022-12-22T02:42:50ZengJosip Juraj Strossmayer University of Osijek, Faculty of Food Technology OsijekCroatian Journal of Food Science and Technology1847-34661848-99232020-01-0112111812210.17508/CJFST.2020.12.1.15238581Phytochemical screening and antioxidant evaluation of millet varieties of PakistanAMBREEN NAZ0ALLAH RUKHA1SHABBIR AHMAD2UMMARA WAHEED3KASHIF RAZZAQ4UMAR FAROOQ5NIGHAT RAZA6NOSHEEN NAZ7Department of Food Science and Technology, Muhammad Nawaz Shareef University of Agriculture, Multan, PakistanNational Institute of Food Science and Technology, Faculty of Food, Nutrition and Home Sciences, University of Agriculture, Faisalabad, PakistanDepartment of Food Science and Technology, Muhammad Nawaz Shareef University of Agriculture, Multan, PakistanInstitute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef University of Agriculture, Multan, PakistanDepartment of Horticulture, Muhammad Nawaz Shareef University of Agriculture, Multan, PakistanDepartment of Food Science and Technology, Muhammad Nawaz Shareef University of Agriculture, Multan, PakistanDepartment of Food Science and Technology, Muhammad Nawaz Shareef University of Agriculture, Multan, PakistanNational Institute of Food Science and Technology, Faculty of Food, Nutrition and Home Sciences, University of Agriculture, Faisalabad, PakistanThe current research was planned to measure the comparative phytochemical and anti-oxidative potential of aqueous and methanolic extracts of two indigenously grown millet varieties of Pakistan. The locally available millet varieties, i.e. MB-87 and AF-POP flours were chemically characterized through the proximate and mineral analysis. The antioxidant extract was prepared in water and methanol. The extracts were screened for saponins, terpenoids, flavonoids and tannins; methanolic extract of MB-87 and AF-POP showed flavonoids in an average quantity (++), whilst aqueous extract had only trace (+) values. The phytochemical screening showed the presence of saponins only in the aqueous extract of both varieties. However, terpenoids and tannins were present in both methanolic and aqueous extracts. During antioxidant evaluation, millet methanolic extracts showed higher level of TPC and DPPH of MB-87 and AF-POP as 0.30±0.001 & 0.23±0.0012 mg GAE/g and 48±0.96 &46±1.09%, respectively. However, the β-carotene activity of the aqueous extract of both varieties (MB-87 and AF-POP) was recorded higher. In a nutshell, the methanolic extract of MB-87 has an appreciable atioxidant profile. Furher research should be planned to screen the important constituents of Pakistani millet varieties.https://hrcak.srce.hr/file/346465
spellingShingle AMBREEN NAZ
ALLAH RUKHA
SHABBIR AHMAD
UMMARA WAHEED
KASHIF RAZZAQ
UMAR FAROOQ
NIGHAT RAZA
NOSHEEN NAZ
Phytochemical screening and antioxidant evaluation of millet varieties of Pakistan
Croatian Journal of Food Science and Technology
title Phytochemical screening and antioxidant evaluation of millet varieties of Pakistan
title_full Phytochemical screening and antioxidant evaluation of millet varieties of Pakistan
title_fullStr Phytochemical screening and antioxidant evaluation of millet varieties of Pakistan
title_full_unstemmed Phytochemical screening and antioxidant evaluation of millet varieties of Pakistan
title_short Phytochemical screening and antioxidant evaluation of millet varieties of Pakistan
title_sort phytochemical screening and antioxidant evaluation of millet varieties of pakistan
url https://hrcak.srce.hr/file/346465
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