Protective effects of Otophylloside N on pentylenetetrazol-induced neuronal injury in vitro and in vivo
Approximately 30% of epileptic patients worldwide are medically unable to control their seizures. In addition, repeated epileptic seizures generally lead to neural damage. Pentylenetetrazol (PTZ) is a clinical circulatory and respiratory stimulant that is experimentally used to mimic epileptic convu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-07-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fphar.2016.00224/full |
| _version_ | 1818594511379496960 |
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| author | Feiya Sheng Mengting Chen Yuan Tan Cheng Xiang Mi Zhang Baocai Li Huanxing Su Chengwei He Jianbo Wan Peng Li |
| author_facet | Feiya Sheng Mengting Chen Yuan Tan Cheng Xiang Mi Zhang Baocai Li Huanxing Su Chengwei He Jianbo Wan Peng Li |
| author_sort | Feiya Sheng |
| collection | DOAJ |
| description | Approximately 30% of epileptic patients worldwide are medically unable to control their seizures. In addition, repeated epileptic seizures generally lead to neural damage. Pentylenetetrazol (PTZ) is a clinical circulatory and respiratory stimulant that is experimentally used to mimic epileptic convulsion in epilepsy research. Here, we systematically explore the neuroprotective effects of a pure compound isolated from Cynanchum otophyllum Schneid (Qingyangshen), Otophylloside N (OtoN), against PTZ-induced neuronal injury. We used three models: in vitro primary cortical neurons, in vivo mice and in vivo zebrafish. Our results revealed that OtoN treatment may attenuate PTZ-induced morphology changes, cell death, LDH efflux in embryonic neuronal cells of C57BL/6J mice, and convulsive behavior in zebrafish. Additionally, our Western blot and RT-PCR results demonstrated that OtoN may attenuate PTZ-induced apoptosis and neuronal activation in neuronal cells, mice and zebrafish. OtoN may reduce PTZ-induced cleavage of poly ADP-ribose polymerase (PARP) and upregulation of the Bax/Bcl-2 ratio and decrease the expression level of c-Fos. This study is the first investigation of the neuroprotective effects of OtoN, which might be developed as a novel antiepileptic drug. |
| first_indexed | 2024-12-16T11:01:17Z |
| format | Article |
| id | doaj.art-7a0fe8c5a9074b1d9b9fb94d40d06d10 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-16T11:01:17Z |
| publishDate | 2016-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-7a0fe8c5a9074b1d9b9fb94d40d06d102022-12-21T22:34:00ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122016-07-01710.3389/fphar.2016.00224209260Protective effects of Otophylloside N on pentylenetetrazol-induced neuronal injury in vitro and in vivoFeiya Sheng0Mengting Chen1Yuan Tan2Cheng Xiang3Mi Zhang4Baocai Li5Huanxing Su6Chengwei He7Jianbo Wan8Peng Li9University of MacauUniversity of MacauUniversity of MacauKunming University of Science and TechnologyKunming University of Science and TechnologyKunming University of Science and TechnologyUniversity of MacauUniversity of MacauUniversity of MacauUniversity of MacauApproximately 30% of epileptic patients worldwide are medically unable to control their seizures. In addition, repeated epileptic seizures generally lead to neural damage. Pentylenetetrazol (PTZ) is a clinical circulatory and respiratory stimulant that is experimentally used to mimic epileptic convulsion in epilepsy research. Here, we systematically explore the neuroprotective effects of a pure compound isolated from Cynanchum otophyllum Schneid (Qingyangshen), Otophylloside N (OtoN), against PTZ-induced neuronal injury. We used three models: in vitro primary cortical neurons, in vivo mice and in vivo zebrafish. Our results revealed that OtoN treatment may attenuate PTZ-induced morphology changes, cell death, LDH efflux in embryonic neuronal cells of C57BL/6J mice, and convulsive behavior in zebrafish. Additionally, our Western blot and RT-PCR results demonstrated that OtoN may attenuate PTZ-induced apoptosis and neuronal activation in neuronal cells, mice and zebrafish. OtoN may reduce PTZ-induced cleavage of poly ADP-ribose polymerase (PARP) and upregulation of the Bax/Bcl-2 ratio and decrease the expression level of c-Fos. This study is the first investigation of the neuroprotective effects of OtoN, which might be developed as a novel antiepileptic drug.http://journal.frontiersin.org/Journal/10.3389/fphar.2016.00224/fullApoptosisEpilepsypentylenetetrazolNeuroprotective effectCynanchum otophyllum SchneidOtophylloside N |
| spellingShingle | Feiya Sheng Mengting Chen Yuan Tan Cheng Xiang Mi Zhang Baocai Li Huanxing Su Chengwei He Jianbo Wan Peng Li Protective effects of Otophylloside N on pentylenetetrazol-induced neuronal injury in vitro and in vivo Frontiers in Pharmacology Apoptosis Epilepsy pentylenetetrazol Neuroprotective effect Cynanchum otophyllum Schneid Otophylloside N |
| title | Protective effects of Otophylloside N on pentylenetetrazol-induced neuronal injury in vitro and in vivo |
| title_full | Protective effects of Otophylloside N on pentylenetetrazol-induced neuronal injury in vitro and in vivo |
| title_fullStr | Protective effects of Otophylloside N on pentylenetetrazol-induced neuronal injury in vitro and in vivo |
| title_full_unstemmed | Protective effects of Otophylloside N on pentylenetetrazol-induced neuronal injury in vitro and in vivo |
| title_short | Protective effects of Otophylloside N on pentylenetetrazol-induced neuronal injury in vitro and in vivo |
| title_sort | protective effects of otophylloside n on pentylenetetrazol induced neuronal injury in vitro and in vivo |
| topic | Apoptosis Epilepsy pentylenetetrazol Neuroprotective effect Cynanchum otophyllum Schneid Otophylloside N |
| url | http://journal.frontiersin.org/Journal/10.3389/fphar.2016.00224/full |
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