Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization
IntroductionRecently, follicle stimulating hormone (FSH) through interaction with its receptor (FSHR) has been proposed to play a role in postmenopausal osteoporosis and cardiovascular disease, rather than the loss of estrogen. To explore this hypothesis, unravelling which cells express extragonadal...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1095031/full |
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| author | Victoria N. Tedjawirja Gerrit K. J. Hooijer C. Dilara Savci-Heijink Kristina Kovac Ron Balm Vivian de Waard |
| author_facet | Victoria N. Tedjawirja Gerrit K. J. Hooijer C. Dilara Savci-Heijink Kristina Kovac Ron Balm Vivian de Waard |
| author_sort | Victoria N. Tedjawirja |
| collection | DOAJ |
| description | IntroductionRecently, follicle stimulating hormone (FSH) through interaction with its receptor (FSHR) has been proposed to play a role in postmenopausal osteoporosis and cardiovascular disease, rather than the loss of estrogen. To explore this hypothesis, unravelling which cells express extragonadal FSHR on protein level is key.MethodsWe used two commercial anti-FSHR antibodies and validated them by performing immunohistochemistry on positive (ovary, testis) and negative controls (skin).ResultsThe monoclonal anti-FSHR antibody could not identify the FSHR in ovary or testis. The polyclonal anti-FSHR antibody stained the granulosa cells (ovary) and Sertoli cells (testis), yet there was equally intense staining of other cells/extracellular matrix. Furthermore, the polyclonal anti-FSHR antibody also stained skin tissue extensively, suggesting that the antibody stains more than just FSHR.DiscussionThe findings in this study may add accuracy to literature on extragonadal FSHR localization and warrants attention to the use of inadequate anti-FSHR antibodies to value the potential role of FSH/FSHR in postmenopausal disease. |
| first_indexed | 2024-04-10T09:54:16Z |
| format | Article |
| id | doaj.art-7a10bad868ab4681b79fadad5e4b2d25 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-10T09:54:16Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-7a10bad868ab4681b79fadad5e4b2d252023-02-16T12:43:34ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-02-011410.3389/fendo.2023.10950311095031Inadequate detection of the FSHR complicates future research on extragonadal FSHR localizationVictoria N. Tedjawirja0Gerrit K. J. Hooijer1C. Dilara Savci-Heijink2Kristina Kovac3Ron Balm4Vivian de Waard5Department of Surgery, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centre (UMC), University of Amsterdam, Amsterdam, NetherlandsDepartment of Pathology, Amsterdam University Medical Centre (UMC), University of Amsterdam, Amsterdam, NetherlandsDepartment of Pathology, Amsterdam University Medical Centre (UMC), University of Amsterdam, Amsterdam, NetherlandsDepartment of Medical Biochemistry, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centre (UMC), University of Amsterdam, Amsterdam, NetherlandsDepartment of Surgery, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centre (UMC), University of Amsterdam, Amsterdam, NetherlandsDepartment of Medical Biochemistry, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centre (UMC), University of Amsterdam, Amsterdam, NetherlandsIntroductionRecently, follicle stimulating hormone (FSH) through interaction with its receptor (FSHR) has been proposed to play a role in postmenopausal osteoporosis and cardiovascular disease, rather than the loss of estrogen. To explore this hypothesis, unravelling which cells express extragonadal FSHR on protein level is key.MethodsWe used two commercial anti-FSHR antibodies and validated them by performing immunohistochemistry on positive (ovary, testis) and negative controls (skin).ResultsThe monoclonal anti-FSHR antibody could not identify the FSHR in ovary or testis. The polyclonal anti-FSHR antibody stained the granulosa cells (ovary) and Sertoli cells (testis), yet there was equally intense staining of other cells/extracellular matrix. Furthermore, the polyclonal anti-FSHR antibody also stained skin tissue extensively, suggesting that the antibody stains more than just FSHR.DiscussionThe findings in this study may add accuracy to literature on extragonadal FSHR localization and warrants attention to the use of inadequate anti-FSHR antibodies to value the potential role of FSH/FSHR in postmenopausal disease.https://www.frontiersin.org/articles/10.3389/fendo.2023.1095031/fullfollicle stimulating hormone receptorextragonadal cellsantibodiesimmunohistochemistrycontrol |
| spellingShingle | Victoria N. Tedjawirja Gerrit K. J. Hooijer C. Dilara Savci-Heijink Kristina Kovac Ron Balm Vivian de Waard Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization Frontiers in Endocrinology follicle stimulating hormone receptor extragonadal cells antibodies immunohistochemistry control |
| title | Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization |
| title_full | Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization |
| title_fullStr | Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization |
| title_full_unstemmed | Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization |
| title_short | Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization |
| title_sort | inadequate detection of the fshr complicates future research on extragonadal fshr localization |
| topic | follicle stimulating hormone receptor extragonadal cells antibodies immunohistochemistry control |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1095031/full |
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