Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm
In this study, the effects of supplemented inositol on sperm extenders were examined on the spermatozoa motility rate and duration, total antioxidant and oxidant status, apoptotic spermatozoa and DNA damage, during the sperm post-thaw process of Mesopotamian Catfish (<i>Silurus triostegus</...
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MDPI AG
2021-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/11/3029 |
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| author | Zafer Doğu Erdinç Şahinöz Faruk Aral İsmail Koyuncu Özgür Yüksekdağ |
| author_facet | Zafer Doğu Erdinç Şahinöz Faruk Aral İsmail Koyuncu Özgür Yüksekdağ |
| author_sort | Zafer Doğu |
| collection | DOAJ |
| description | In this study, the effects of supplemented inositol on sperm extenders were examined on the spermatozoa motility rate and duration, total antioxidant and oxidant status, apoptotic spermatozoa and DNA damage, during the sperm post-thaw process of Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843). The semen was frozen in diluents containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly improved the spermatozoa motility rate and duration significantly (<i>p</i> < 0.05). MDA and TOS were linearly decreased, however, TAS and GSH linearly increased (<i>p</i> < 0.05). The increasing inositol levels resulted in a linear and quadratic decrease in DNA damage in the comet assay, 8-hydroxydeoxyguanosine and the determined percentage of apoptotic spermatozoa (<i>p</i> < 0.05). These results suggest that there are many positive effects of the use of supplemental inositol on enhancing sperm cryopreservation efficiency in <i>Silurus triostegus</i>. |
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| id | doaj.art-7a11d84c6ea2422d9d98e841ab333066 |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T05:47:08Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj.art-7a11d84c6ea2422d9d98e841ab3330662023-11-22T22:05:27ZengMDPI AGAnimals2076-26152021-10-011111302910.3390/ani11113029Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) SpermZafer Doğu0Erdinç Şahinöz1Faruk Aral2İsmail Koyuncu3Özgür Yüksekdağ4Department of Fisheries and Aquaculture, Bozova Vocational High School, Harran University, Bozova 63850, TurkeyDepartment of Fisheries and Aquaculture, Bozova Vocational High School, Harran University, Bozova 63850, TurkeyÇarşı PTT PK 138, Konya 42010, TurkeyDepartment of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa 63050, TurkeyDepartment of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa 63050, TurkeyIn this study, the effects of supplemented inositol on sperm extenders were examined on the spermatozoa motility rate and duration, total antioxidant and oxidant status, apoptotic spermatozoa and DNA damage, during the sperm post-thaw process of Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843). The semen was frozen in diluents containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly improved the spermatozoa motility rate and duration significantly (<i>p</i> < 0.05). MDA and TOS were linearly decreased, however, TAS and GSH linearly increased (<i>p</i> < 0.05). The increasing inositol levels resulted in a linear and quadratic decrease in DNA damage in the comet assay, 8-hydroxydeoxyguanosine and the determined percentage of apoptotic spermatozoa (<i>p</i> < 0.05). These results suggest that there are many positive effects of the use of supplemental inositol on enhancing sperm cryopreservation efficiency in <i>Silurus triostegus</i>.https://www.mdpi.com/2076-2615/11/11/3029apoptosiscryopreservationDNA damageoxidative stress<i>Silurus triostegus</i>sperm |
| spellingShingle | Zafer Doğu Erdinç Şahinöz Faruk Aral İsmail Koyuncu Özgür Yüksekdağ Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm Animals apoptosis cryopreservation DNA damage oxidative stress <i>Silurus triostegus</i> sperm |
| title | Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm |
| title_full | Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm |
| title_fullStr | Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm |
| title_full_unstemmed | Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm |
| title_short | Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (<i>Silurus triostegus</i>, H. 1843) Sperm |
| title_sort | effects of inositol supplementation in sperm extender on the quality of cryopreserved mesopotamian catfish i silurus triostegus i h 1843 sperm |
| topic | apoptosis cryopreservation DNA damage oxidative stress <i>Silurus triostegus</i> sperm |
| url | https://www.mdpi.com/2076-2615/11/11/3029 |
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