Effect of mucosal adjuvant IL-1β on heterotypic immunity in a pig influenza model
T cell responses directed against highly conserved viral proteins contribute to the clearance of the influenza virus and confer broadly cross-reactive and protective immune responses against a range of influenza viruses in mice and ferrets. We examined the protective efficacy of mucosal delivery of...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1181716/full |
| _version_ | 1827964175500967936 |
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| author | Anna Schmidt Basudev Paudyal Sonia Villanueva-Hernández Adam Mcnee Eleni Vatzia Brigid Veronica Carr Selma Schmidt Amy Mccarron Veronica Martini Silke Schroedel Christian Thirion Ryan Waters Francisco J. Salguero Wilhelm Gerner Matthias Tenbusch Matthias Tenbusch Elma Tchilian |
| author_facet | Anna Schmidt Basudev Paudyal Sonia Villanueva-Hernández Adam Mcnee Eleni Vatzia Brigid Veronica Carr Selma Schmidt Amy Mccarron Veronica Martini Silke Schroedel Christian Thirion Ryan Waters Francisco J. Salguero Wilhelm Gerner Matthias Tenbusch Matthias Tenbusch Elma Tchilian |
| author_sort | Anna Schmidt |
| collection | DOAJ |
| description | T cell responses directed against highly conserved viral proteins contribute to the clearance of the influenza virus and confer broadly cross-reactive and protective immune responses against a range of influenza viruses in mice and ferrets. We examined the protective efficacy of mucosal delivery of adenoviral vectors expressing hemagglutinin (HA) and nucleoprotein (NP) from the H1N1 virus against heterologous H3N2 challenge in pigs. We also evaluated the effect of mucosal co-delivery of IL-1β, which significantly increased antibody and T cell responses in inbred Babraham pigs. Another group of outbred pigs was first exposed to pH1N1 as an alternative means of inducing heterosubtypic immunity and were subsequently challenged with H3N2. Although both prior infection and adenoviral vector immunization induced strong T-cell responses against the conserved NP protein, none of the treatment groups demonstrated increased protection against the heterologous H3N2 challenge. Ad-HA/NP+Ad-IL-1β immunization increased lung pathology, although viral load was unchanged. These data indicate that heterotypic immunity may be difficult to achieve in pigs and the immunological mechanisms may differ from those in small animal models. Caution should be applied in extrapolating from a single model to humans. |
| first_indexed | 2024-04-09T17:11:34Z |
| format | Article |
| id | doaj.art-7a124e0b2d554bd1b327bae9c7f98cfe |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-09T17:11:34Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-7a124e0b2d554bd1b327bae9c7f98cfe2023-04-20T05:58:41ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-04-011410.3389/fimmu.2023.11817161181716Effect of mucosal adjuvant IL-1β on heterotypic immunity in a pig influenza modelAnna Schmidt0Basudev Paudyal1Sonia Villanueva-Hernández2Adam Mcnee3Eleni Vatzia4Brigid Veronica Carr5Selma Schmidt6Amy Mccarron7Veronica Martini8Silke Schroedel9Christian Thirion10Ryan Waters11Francisco J. Salguero12Wilhelm Gerner13Matthias Tenbusch14Matthias Tenbusch15Elma Tchilian16Virologisches Institut-Klinische und Molekulare Virologie, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, GermanyHost Responses, The Pirbright Institute, Pirbright, United KingdomHost Responses, The Pirbright Institute, Pirbright, United KingdomHost Responses, The Pirbright Institute, Pirbright, United KingdomHost Responses, The Pirbright Institute, Pirbright, United KingdomHost Responses, The Pirbright Institute, Pirbright, United KingdomHost Responses, The Pirbright Institute, Pirbright, United KingdomHost Responses, The Pirbright Institute, Pirbright, United KingdomInstitute for Research in Biomedicine, Bellinzona, SwitzerlandSIRION Biotech GmbH, Martinsried, GermanySIRION Biotech GmbH, Martinsried, GermanyHost Responses, The Pirbright Institute, Pirbright, United KingdomUnited Kingdom Health Security Agency, UKHSA-Porton Down, Salisbury, United KingdomHost Responses, The Pirbright Institute, Pirbright, United KingdomVirologisches Institut-Klinische und Molekulare Virologie, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, GermanyMedical Immunology Campus Erlangen (MICE), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, GermanyHost Responses, The Pirbright Institute, Pirbright, United KingdomT cell responses directed against highly conserved viral proteins contribute to the clearance of the influenza virus and confer broadly cross-reactive and protective immune responses against a range of influenza viruses in mice and ferrets. We examined the protective efficacy of mucosal delivery of adenoviral vectors expressing hemagglutinin (HA) and nucleoprotein (NP) from the H1N1 virus against heterologous H3N2 challenge in pigs. We also evaluated the effect of mucosal co-delivery of IL-1β, which significantly increased antibody and T cell responses in inbred Babraham pigs. Another group of outbred pigs was first exposed to pH1N1 as an alternative means of inducing heterosubtypic immunity and were subsequently challenged with H3N2. Although both prior infection and adenoviral vector immunization induced strong T-cell responses against the conserved NP protein, none of the treatment groups demonstrated increased protection against the heterologous H3N2 challenge. Ad-HA/NP+Ad-IL-1β immunization increased lung pathology, although viral load was unchanged. These data indicate that heterotypic immunity may be difficult to achieve in pigs and the immunological mechanisms may differ from those in small animal models. Caution should be applied in extrapolating from a single model to humans.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1181716/fullinfluenza viruspigmucosal immunityIL-1βheterotypic protectionporcine B cells |
| spellingShingle | Anna Schmidt Basudev Paudyal Sonia Villanueva-Hernández Adam Mcnee Eleni Vatzia Brigid Veronica Carr Selma Schmidt Amy Mccarron Veronica Martini Silke Schroedel Christian Thirion Ryan Waters Francisco J. Salguero Wilhelm Gerner Matthias Tenbusch Matthias Tenbusch Elma Tchilian Effect of mucosal adjuvant IL-1β on heterotypic immunity in a pig influenza model Frontiers in Immunology influenza virus pig mucosal immunity IL-1β heterotypic protection porcine B cells |
| title | Effect of mucosal adjuvant IL-1β on heterotypic immunity in a pig influenza model |
| title_full | Effect of mucosal adjuvant IL-1β on heterotypic immunity in a pig influenza model |
| title_fullStr | Effect of mucosal adjuvant IL-1β on heterotypic immunity in a pig influenza model |
| title_full_unstemmed | Effect of mucosal adjuvant IL-1β on heterotypic immunity in a pig influenza model |
| title_short | Effect of mucosal adjuvant IL-1β on heterotypic immunity in a pig influenza model |
| title_sort | effect of mucosal adjuvant il 1β on heterotypic immunity in a pig influenza model |
| topic | influenza virus pig mucosal immunity IL-1β heterotypic protection porcine B cells |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1181716/full |
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