Stripe rust resistance gene(s) postulation in wheat germplasm with the help of differentials and tagged molecular markers
Abstract Thirteen known Yr gene-associated markers pertaining to genes (Yr5, Yr10, Yr15, Yr24/Yr26) were used to identify the genes in selected wheat germplasm which were found resistant under field conditions at two locations in Punjab, India against stripe rust. In field evaluation, 38 genotypes e...
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-36197-y |
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| author | Mohammad Waris Haider Jaspal Kaur Ritu Bala Sandeep Singh Puja Srivastava Achla Sharma Rohtas Singh Jyoti Kumari |
| author_facet | Mohammad Waris Haider Jaspal Kaur Ritu Bala Sandeep Singh Puja Srivastava Achla Sharma Rohtas Singh Jyoti Kumari |
| author_sort | Mohammad Waris Haider |
| collection | DOAJ |
| description | Abstract Thirteen known Yr gene-associated markers pertaining to genes (Yr5, Yr10, Yr15, Yr24/Yr26) were used to identify the genes in selected wheat germplasm which were found resistant under field conditions at two locations in Punjab, India against stripe rust. In field evaluation, 38 genotypes exhibited highly resistant response, with a final rust severity (FRS) ranging from 0 to TR. Seven genotypes expressed a resistant to moderately resistant response with FRS ranging from 5MR–10S. In race-specific phenotying against most prevalent pathotypes of Puccinia striiformis tritici (46S119,110S119 &238S119) by seedling reaction test (SRT) 14 genotypes (29.2%) were found to be immune (IT = 0), 28 genotypes (58.3%) were resistant (IT = 1), and 3 genotypes (6.3%) were moderately resistant (IT = 2). Yr5 was detected in sixteen lines with the help of two markers Xwmc175 and Xgwm120 linked with Yr5. Yr10 was detected in ten lines with the marker Xpsp3000 and Yr15 was detected in fourteen lines with two linked markers; Xgwm413 and Xgwm273. Likewise, Yr24/26 was detected in 15 lines with two linked markers, namely Xbarc181 and Xbarc187. Based on the race specific phenotyping data and marker data, fourteen lines were found to carry a single gene, 16 showed the presence of two gene combinations, and seven genotypes were found to have a combination of three genes. Frequencies of Yr5, Yr15 and Yr26/Yr24 was high among test wheat germplasm in comparison to Yr10. |
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| language | English |
| last_indexed | 2024-03-13T03:22:15Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-7a129c123314470c921f950d99d1fbad2023-06-25T11:17:35ZengNature PortfolioScientific Reports2045-23222023-06-0113111410.1038/s41598-023-36197-yStripe rust resistance gene(s) postulation in wheat germplasm with the help of differentials and tagged molecular markersMohammad Waris Haider0Jaspal Kaur1Ritu Bala2Sandeep Singh3Puja Srivastava4Achla Sharma5Rohtas Singh6Jyoti Kumari7Department of Plant Pathology, PAUDepartment of Plant Breeding and Genetics, PAUDepartment of Plant Breeding and Genetics, PAUDepartment of Plant Pathology, PAUDepartment of Plant Breeding and Genetics, PAUDepartment of Plant Breeding and Genetics, PAUDepartment of Plant Breeding and Genetics, PAUNational Bureau of Plant Genetic ResourcesAbstract Thirteen known Yr gene-associated markers pertaining to genes (Yr5, Yr10, Yr15, Yr24/Yr26) were used to identify the genes in selected wheat germplasm which were found resistant under field conditions at two locations in Punjab, India against stripe rust. In field evaluation, 38 genotypes exhibited highly resistant response, with a final rust severity (FRS) ranging from 0 to TR. Seven genotypes expressed a resistant to moderately resistant response with FRS ranging from 5MR–10S. In race-specific phenotying against most prevalent pathotypes of Puccinia striiformis tritici (46S119,110S119 &238S119) by seedling reaction test (SRT) 14 genotypes (29.2%) were found to be immune (IT = 0), 28 genotypes (58.3%) were resistant (IT = 1), and 3 genotypes (6.3%) were moderately resistant (IT = 2). Yr5 was detected in sixteen lines with the help of two markers Xwmc175 and Xgwm120 linked with Yr5. Yr10 was detected in ten lines with the marker Xpsp3000 and Yr15 was detected in fourteen lines with two linked markers; Xgwm413 and Xgwm273. Likewise, Yr24/26 was detected in 15 lines with two linked markers, namely Xbarc181 and Xbarc187. Based on the race specific phenotyping data and marker data, fourteen lines were found to carry a single gene, 16 showed the presence of two gene combinations, and seven genotypes were found to have a combination of three genes. Frequencies of Yr5, Yr15 and Yr26/Yr24 was high among test wheat germplasm in comparison to Yr10.https://doi.org/10.1038/s41598-023-36197-y |
| spellingShingle | Mohammad Waris Haider Jaspal Kaur Ritu Bala Sandeep Singh Puja Srivastava Achla Sharma Rohtas Singh Jyoti Kumari Stripe rust resistance gene(s) postulation in wheat germplasm with the help of differentials and tagged molecular markers Scientific Reports |
| title | Stripe rust resistance gene(s) postulation in wheat germplasm with the help of differentials and tagged molecular markers |
| title_full | Stripe rust resistance gene(s) postulation in wheat germplasm with the help of differentials and tagged molecular markers |
| title_fullStr | Stripe rust resistance gene(s) postulation in wheat germplasm with the help of differentials and tagged molecular markers |
| title_full_unstemmed | Stripe rust resistance gene(s) postulation in wheat germplasm with the help of differentials and tagged molecular markers |
| title_short | Stripe rust resistance gene(s) postulation in wheat germplasm with the help of differentials and tagged molecular markers |
| title_sort | stripe rust resistance gene s postulation in wheat germplasm with the help of differentials and tagged molecular markers |
| url | https://doi.org/10.1038/s41598-023-36197-y |
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