Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates
Equid herpesviruses (EHVs) affect equine health and can cause significant economic losses to the equine industry worldwide. In the current study, the circulation of two infectious equid herpesviruses (EHV-1 and EHV-4) among different horse populations in some farms was monitored. In the present study...
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| Format: | Article |
| Language: | English |
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Egyptian Society for Animal Management
2021-01-01
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| Series: | Journal of Applied Veterinary Sciences |
| Subjects: | |
| Online Access: | https://javs.journals.ekb.eg/article_140052.html |
| _version_ | 1818839656503967744 |
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| author | Morcos Ibrahim Yanni Ebtsam, A. Abouelyazeed Hala Abdelrehim Ali Nadia Maher Hanna |
| author_facet | Morcos Ibrahim Yanni Ebtsam, A. Abouelyazeed Hala Abdelrehim Ali Nadia Maher Hanna |
| author_sort | Morcos Ibrahim Yanni |
| collection | DOAJ |
| description | Equid herpesviruses (EHVs) affect equine health and can cause significant economic losses to the equine industry worldwide. In the current study, the circulation of two infectious equid herpesviruses (EHV-1 and EHV-4) among different horse populations in some farms was monitored. In the present study, 50 samples of nasal secretions and tissue homogenates from neurological disease cases, abortion, neonatal foal deaths, and 36 serum samples. Samples of swabs and organs inoculated in embryonated chiken egg and Madin darby bovine kidney cell line. 29samples were positive in egg injection but no detected CPE in cell line for three passages. DNA was extracted and subjected to conventional PCR to detect the two herpesviruses' presence using specific primers. Three isolates of EHV-1 and four were detected. One EHV-1 and two EHV-4 were subjected to phylogenetic analysis. Phylogenetic analysis confirmed the existence of the isolated EHV-1 and EHV-4. They were more closely related to other previously isolated EHV-1 and EHV-4 from Egypt and other countries. Antibodies against EHV-1 and EHV-4 were tested using ELISA. The results showed that EHV-1 and EHV-4 are endemic and can be a continuous threat for horses in the absence of vaccination programs and frequent virus reactivation. |
| first_indexed | 2024-12-19T03:57:46Z |
| format | Article |
| id | doaj.art-7a135de79e4d4ca8a23a645b84a5af80 |
| institution | Directory Open Access Journal |
| issn | 1687-4072 2090-3308 |
| language | English |
| last_indexed | 2024-12-19T03:57:46Z |
| publishDate | 2021-01-01 |
| publisher | Egyptian Society for Animal Management |
| record_format | Article |
| series | Journal of Applied Veterinary Sciences |
| spelling | doaj.art-7a135de79e4d4ca8a23a645b84a5af802022-12-21T20:36:46ZengEgyptian Society for Animal ManagementJournal of Applied Veterinary Sciences1687-40722090-33082021-01-016118https://dx.doi.org/10.21608/javs.2021.140052Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian GovernoratesMorcos Ibrahim Yanni0Ebtsam, A. Abouelyazeed1Hala Abdelrehim Ali2Nadia Maher Hanna3Virology Department, Animal Health Research Institute , Dokki, Agriculture Research Centre (ARC), Egypt.Virology Department, Animal Health Research Institute , Dokki, Agriculture Research Centre (ARC), Egypt.Virology Department, Animal Health Research Institute , Dokki, Agriculture Research Centre (ARC), Egypt.Virology Department, Animal Health Research Institute , Dokki, Agriculture Research Centre (ARC), Egypt.Equid herpesviruses (EHVs) affect equine health and can cause significant economic losses to the equine industry worldwide. In the current study, the circulation of two infectious equid herpesviruses (EHV-1 and EHV-4) among different horse populations in some farms was monitored. In the present study, 50 samples of nasal secretions and tissue homogenates from neurological disease cases, abortion, neonatal foal deaths, and 36 serum samples. Samples of swabs and organs inoculated in embryonated chiken egg and Madin darby bovine kidney cell line. 29samples were positive in egg injection but no detected CPE in cell line for three passages. DNA was extracted and subjected to conventional PCR to detect the two herpesviruses' presence using specific primers. Three isolates of EHV-1 and four were detected. One EHV-1 and two EHV-4 were subjected to phylogenetic analysis. Phylogenetic analysis confirmed the existence of the isolated EHV-1 and EHV-4. They were more closely related to other previously isolated EHV-1 and EHV-4 from Egypt and other countries. Antibodies against EHV-1 and EHV-4 were tested using ELISA. The results showed that EHV-1 and EHV-4 are endemic and can be a continuous threat for horses in the absence of vaccination programs and frequent virus reactivation.https://javs.journals.ekb.eg/article_140052.htmldnaelisaehv-1equineherpesvirus |
| spellingShingle | Morcos Ibrahim Yanni Ebtsam, A. Abouelyazeed Hala Abdelrehim Ali Nadia Maher Hanna Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates Journal of Applied Veterinary Sciences dna elisa ehv-1 equine herpesvirus |
| title | Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates |
| title_full | Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates |
| title_fullStr | Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates |
| title_full_unstemmed | Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates |
| title_short | Verification Of Molecular And Conventional Techniques Used In The Diagnosis Of Equine Herpes Virus In Some Egyptian Governorates |
| title_sort | verification of molecular and conventional techniques used in the diagnosis of equine herpes virus in some egyptian governorates |
| topic | dna elisa ehv-1 equine herpesvirus |
| url | https://javs.journals.ekb.eg/article_140052.html |
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